Molecular characterization and genotyping of hepatitis C virus from Sudanese end-stage renal disease patients on haemodialysis
Abstract Background Hepatitis C virus (HCV) is a global public health problem, with ~ 11 million people in Africa infected. There is incomplete information on HCV in Sudan, particularly in haemodialysis patients, who have a higher prevalence compared to the general population. Thus, our objectives w...
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BMC
2022-11-01
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Series: | BMC Infectious Diseases |
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Online Access: | https://doi.org/10.1186/s12879-022-07833-1 |
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author | Trodia Zitha Chien-Yu Chen Hatim Mudawi Waleed Hussein Maowia Mukhtar Mazin Shigidi Mohamed Elamin Awad Yousif Mohammed Ahmed Ali Dieter Glebe Anna Kramvis |
author_facet | Trodia Zitha Chien-Yu Chen Hatim Mudawi Waleed Hussein Maowia Mukhtar Mazin Shigidi Mohamed Elamin Awad Yousif Mohammed Ahmed Ali Dieter Glebe Anna Kramvis |
author_sort | Trodia Zitha |
collection | DOAJ |
description | Abstract Background Hepatitis C virus (HCV) is a global public health problem, with ~ 11 million people in Africa infected. There is incomplete information on HCV in Sudan, particularly in haemodialysis patients, who have a higher prevalence compared to the general population. Thus, our objectives were to genotype and molecularly characterize HCV isolated from end-stage renal disease haemodialysis patients. Methods A total of 541 patients were recruited from eight haemodialysis centres in Khartoum and screened for anti-HCV. Viral loads were determined using in-house real-time PCR in seropositive patients. HCV was genotyped and subtyped using sequencing of amplicons of 5′ untranslated (UTR) and non-structural protein 5B (NS5B) regions, followed by phylogenetic analysis of corresponding sequences. Results The HCV seroprevalence in the study was 17% (93/541), with HCV RNA-positive viremic rate of 7% (40/541). A low HCV load, with a mean of 2.85 × 104 IU/ml and a range of 2.95 × 103 to 4.78 × 106 IU/ml, was detected. Phylogenetic analyses showed the presence of genotypes 1, 3, 4, and 5 with subtypes 1a, 1b, 1 g, 3a, 4a, 4 l, 4 m, 4 s, and 4t. Sequences of HCV from the same haemodialysis units, clustered in similar genotypes and subtypes intimating nosocomial infection. Conclusion HCV infection is highly prevalent in haemodialysis patients from Sudan, with phylogenetic analysis intimating nosocomial infection. HCV genotyping is useful to locate potential transmission chains and to enable individualized treatment using highly effective direct-acting antivirals (DAAs). |
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spelling | doaj.art-8906a79713334cb39b9250afcd1a5f2d2022-12-22T04:18:21ZengBMCBMC Infectious Diseases1471-23342022-11-0122111110.1186/s12879-022-07833-1Molecular characterization and genotyping of hepatitis C virus from Sudanese end-stage renal disease patients on haemodialysisTrodia Zitha0Chien-Yu Chen1Hatim Mudawi2Waleed Hussein3Maowia Mukhtar4Mazin Shigidi5Mohamed Elamin Awad Yousif6Mohammed Ahmed Ali7Dieter Glebe8Anna Kramvis9Hepatitis Virus Diversity Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of the WitwatersrandHepatitis Virus Diversity Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of the WitwatersrandDepartment of Medicine, Faculty of Medicine, University of KhartoumDepartment of Immunology, Faculty of Medical Laboratory Sciences, Al-Neelain UniversityDepartment of Molecular Biology, Institute of Endemic Diseases, University of KhartoumDr. Salma Centre for Dialysis and Kidney Diseases and TransplantationDepartment of Nephrology, Ibn Sina Specialized HospitalAlnaw Teaching HospitalInstitute of Medical Virology, National Reference Centre of Hepatitis B viruses and Hepatitis D viruses, Justus Liebig University of GiessenHepatitis Virus Diversity Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of the WitwatersrandAbstract Background Hepatitis C virus (HCV) is a global public health problem, with ~ 11 million people in Africa infected. There is incomplete information on HCV in Sudan, particularly in haemodialysis patients, who have a higher prevalence compared to the general population. Thus, our objectives were to genotype and molecularly characterize HCV isolated from end-stage renal disease haemodialysis patients. Methods A total of 541 patients were recruited from eight haemodialysis centres in Khartoum and screened for anti-HCV. Viral loads were determined using in-house real-time PCR in seropositive patients. HCV was genotyped and subtyped using sequencing of amplicons of 5′ untranslated (UTR) and non-structural protein 5B (NS5B) regions, followed by phylogenetic analysis of corresponding sequences. Results The HCV seroprevalence in the study was 17% (93/541), with HCV RNA-positive viremic rate of 7% (40/541). A low HCV load, with a mean of 2.85 × 104 IU/ml and a range of 2.95 × 103 to 4.78 × 106 IU/ml, was detected. Phylogenetic analyses showed the presence of genotypes 1, 3, 4, and 5 with subtypes 1a, 1b, 1 g, 3a, 4a, 4 l, 4 m, 4 s, and 4t. Sequences of HCV from the same haemodialysis units, clustered in similar genotypes and subtypes intimating nosocomial infection. Conclusion HCV infection is highly prevalent in haemodialysis patients from Sudan, with phylogenetic analysis intimating nosocomial infection. HCV genotyping is useful to locate potential transmission chains and to enable individualized treatment using highly effective direct-acting antivirals (DAAs).https://doi.org/10.1186/s12879-022-07833-1Hepatitis C virusGenotype/subgenotypeEnd-stage renal diseaseHaemodialysisNosocomial infection |
spellingShingle | Trodia Zitha Chien-Yu Chen Hatim Mudawi Waleed Hussein Maowia Mukhtar Mazin Shigidi Mohamed Elamin Awad Yousif Mohammed Ahmed Ali Dieter Glebe Anna Kramvis Molecular characterization and genotyping of hepatitis C virus from Sudanese end-stage renal disease patients on haemodialysis BMC Infectious Diseases Hepatitis C virus Genotype/subgenotype End-stage renal disease Haemodialysis Nosocomial infection |
title | Molecular characterization and genotyping of hepatitis C virus from Sudanese end-stage renal disease patients on haemodialysis |
title_full | Molecular characterization and genotyping of hepatitis C virus from Sudanese end-stage renal disease patients on haemodialysis |
title_fullStr | Molecular characterization and genotyping of hepatitis C virus from Sudanese end-stage renal disease patients on haemodialysis |
title_full_unstemmed | Molecular characterization and genotyping of hepatitis C virus from Sudanese end-stage renal disease patients on haemodialysis |
title_short | Molecular characterization and genotyping of hepatitis C virus from Sudanese end-stage renal disease patients on haemodialysis |
title_sort | molecular characterization and genotyping of hepatitis c virus from sudanese end stage renal disease patients on haemodialysis |
topic | Hepatitis C virus Genotype/subgenotype End-stage renal disease Haemodialysis Nosocomial infection |
url | https://doi.org/10.1186/s12879-022-07833-1 |
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