Protective effects of polysaccharides from Sipunculus nudus on Beagle dogs exposed to γ-radiation.
The aim of the study is to investigate the radioprotective effect of polysaccharide extract from Sipunculus nudus (SNP). Beagle dogs were randomly divided into the following six groups. Group-1: Un-treated and un-irradiated controls. Group-2: Exposed to a single acute dose of 2 Gy γ-radiation alone....
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4122488?pdf=render |
| _version_ | 1818312946249367552 |
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| author | Fengmei Cui Ming Li Yuejin Chen Yuming Liu Yin He Dingwen Jiang Jian Tong Jianxiang Li Xianrong Shen |
| author_facet | Fengmei Cui Ming Li Yuejin Chen Yuming Liu Yin He Dingwen Jiang Jian Tong Jianxiang Li Xianrong Shen |
| author_sort | Fengmei Cui |
| collection | DOAJ |
| description | The aim of the study is to investigate the radioprotective effect of polysaccharide extract from Sipunculus nudus (SNP). Beagle dogs were randomly divided into the following six groups. Group-1: Un-treated and un-irradiated controls. Group-2: Exposed to a single acute dose of 2 Gy γ-radiation alone. Groups-3, 4 and 5: Oral administration of SNP at 50, 100 or 200 mg/kg body weight once a day for 7 days followed by a single acute whole body exposure to 2 Gy γ-radiation. The same doses of SNP were administered for further 27 days. Group-6: Positive controls treated with 1.6 mg/kg Nilestriol by gavage after radiation. Blood parameters including white/red cells and platelet counts, as well as hemoglobin level, were assessed every other day for 34 days (7 days before and 27 days of experiment). Serum separated from aliquots of the same blood sample was used to estimate enzyme activity of antioxidant superoxide-dismutase, and to determine levels of free radical, nitric oxide, hydroxyl and superoxide anion. At the end of the experiment, all dogs were euthanized to weigh the organs for organ co-efficient calculation. Pathological changes were assessed in the bone marrow. The results showed that the dogs exposed to γ-radiation alone exhibited a typical hematopoietic syndrome. In contrast, at the end of 27 days experiment, dogs received oral administration of SNP+γ-radiation showed: (i) a much improved blood picture as indicated by shorter duration of leucopenia, neutropenia, thrombocytopenia (platelet counts), as well as hemoglobin levels, (ii) significantly improved hematopoietic activity in the bone marrow, (iii) substantial decrease in nitric oxide levels, and notable increase in activity of antioxidant superoxide dismutase. The results suggested that oral administration of SNP in Beagle dogs was effective in facilitating the recovery of hematopoietic bone marrow damage induced by γ-radiation. |
| first_indexed | 2024-12-13T08:25:56Z |
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| id | doaj.art-8908e2cd68ad441e8e6ccf1056be3cb6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T08:25:56Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8908e2cd68ad441e8e6ccf1056be3cb62022-12-21T23:53:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10429910.1371/journal.pone.0104299Protective effects of polysaccharides from Sipunculus nudus on Beagle dogs exposed to γ-radiation.Fengmei CuiMing LiYuejin ChenYuming LiuYin HeDingwen JiangJian TongJianxiang LiXianrong ShenThe aim of the study is to investigate the radioprotective effect of polysaccharide extract from Sipunculus nudus (SNP). Beagle dogs were randomly divided into the following six groups. Group-1: Un-treated and un-irradiated controls. Group-2: Exposed to a single acute dose of 2 Gy γ-radiation alone. Groups-3, 4 and 5: Oral administration of SNP at 50, 100 or 200 mg/kg body weight once a day for 7 days followed by a single acute whole body exposure to 2 Gy γ-radiation. The same doses of SNP were administered for further 27 days. Group-6: Positive controls treated with 1.6 mg/kg Nilestriol by gavage after radiation. Blood parameters including white/red cells and platelet counts, as well as hemoglobin level, were assessed every other day for 34 days (7 days before and 27 days of experiment). Serum separated from aliquots of the same blood sample was used to estimate enzyme activity of antioxidant superoxide-dismutase, and to determine levels of free radical, nitric oxide, hydroxyl and superoxide anion. At the end of the experiment, all dogs were euthanized to weigh the organs for organ co-efficient calculation. Pathological changes were assessed in the bone marrow. The results showed that the dogs exposed to γ-radiation alone exhibited a typical hematopoietic syndrome. In contrast, at the end of 27 days experiment, dogs received oral administration of SNP+γ-radiation showed: (i) a much improved blood picture as indicated by shorter duration of leucopenia, neutropenia, thrombocytopenia (platelet counts), as well as hemoglobin levels, (ii) significantly improved hematopoietic activity in the bone marrow, (iii) substantial decrease in nitric oxide levels, and notable increase in activity of antioxidant superoxide dismutase. The results suggested that oral administration of SNP in Beagle dogs was effective in facilitating the recovery of hematopoietic bone marrow damage induced by γ-radiation.http://europepmc.org/articles/PMC4122488?pdf=render |
| spellingShingle | Fengmei Cui Ming Li Yuejin Chen Yuming Liu Yin He Dingwen Jiang Jian Tong Jianxiang Li Xianrong Shen Protective effects of polysaccharides from Sipunculus nudus on Beagle dogs exposed to γ-radiation. PLoS ONE |
| title | Protective effects of polysaccharides from Sipunculus nudus on Beagle dogs exposed to γ-radiation. |
| title_full | Protective effects of polysaccharides from Sipunculus nudus on Beagle dogs exposed to γ-radiation. |
| title_fullStr | Protective effects of polysaccharides from Sipunculus nudus on Beagle dogs exposed to γ-radiation. |
| title_full_unstemmed | Protective effects of polysaccharides from Sipunculus nudus on Beagle dogs exposed to γ-radiation. |
| title_short | Protective effects of polysaccharides from Sipunculus nudus on Beagle dogs exposed to γ-radiation. |
| title_sort | protective effects of polysaccharides from sipunculus nudus on beagle dogs exposed to γ radiation |
| url | http://europepmc.org/articles/PMC4122488?pdf=render |
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