Diversity of Non-O157 Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Cattle from Central and Southern Chile
Cattle are the main reservoir of Shiga toxin-producing <i>Escherichia coli</i> (STEC), one of the world’s most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces fr...
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MDPI AG
2021-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/8/2388 |
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| author | Leonela Díaz Sebastian Gutierrez Andrea I Moreno-Switt Luis Pablo Hervé Christopher Hamilton-West Nora Lía Padola Paola Navarrete Angélica Reyes-Jara Jianghong Meng Narjol González-Escalona Magaly Toro |
| author_facet | Leonela Díaz Sebastian Gutierrez Andrea I Moreno-Switt Luis Pablo Hervé Christopher Hamilton-West Nora Lía Padola Paola Navarrete Angélica Reyes-Jara Jianghong Meng Narjol González-Escalona Magaly Toro |
| author_sort | Leonela Díaz |
| collection | DOAJ |
| description | Cattle are the main reservoir of Shiga toxin-producing <i>Escherichia coli</i> (STEC), one of the world’s most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces from central and southern Chile. We analyzed 446 cattle fecal samples and isolated non-O157 STEC from 12.6% (56/446); a total of 93 different isolates were recovered. Most isolates displayed β-glucuronidase activity (96.8%; 90/93) and fermented sorbitol (86.0%; 80/93), whereas only 39.8% (37/93) were resistant to tellurite. A subgroup of 30 representative non-O157 STEC isolates was selected for whole-genome sequencing and bioinformatics analysis. In silico analysis showed that they grouped into 16 different sequence types and 17 serotypes; the serotypes most frequently identified were O116:H21 and O168:H8 (13% each). A single isolate of serotype O26:H11 was recovered. One isolate was resistant to tetracycline and carried resistance genes <i>tet(A)</i> and <i>tet(R)</i>; no other isolate displayed antimicrobial resistance or carried antimicrobial resistance genes. The intimin gene (<i>eae</i>) was identified in 13.3% (4/30) of the genomes and 90% (27/30) carried the <i>stx2</i> gene. A phylogenetic reconstruction demonstrated that the isolates clustered based on serotypes, independent of geographical origin. These results indicate that cattle in Chile carry a wide diversity of STEC potentially pathogenic for humans based on the presence of critical virulence genes. |
| first_indexed | 2024-03-10T09:04:26Z |
| format | Article |
| id | doaj.art-890ace1cab3f4f26984430819ad012c7 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T09:04:26Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-890ace1cab3f4f26984430819ad012c72023-11-22T06:31:24ZengMDPI AGAnimals2076-26152021-08-01118238810.3390/ani11082388Diversity of Non-O157 Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Cattle from Central and Southern ChileLeonela Díaz0Sebastian Gutierrez1Andrea I Moreno-Switt2Luis Pablo Hervé3Christopher Hamilton-West4Nora Lía Padola5Paola Navarrete6Angélica Reyes-Jara7Jianghong Meng8Narjol González-Escalona9Magaly Toro10Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Macul 7830490, Santiago, ChileInstituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Macul 7830490, Santiago, ChileEscuela de Medicina Veterinaria, Facultad de Agronomía e Ingeniería Forestal, Facultad de Ciencias Biológicas, Facultad de Medicina, Pontificia Universidad Católica de Chile, Macul 7820244, Santiago, ChileFacultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, La Pintana 8820808, Santiago, ChileFacultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, La Pintana 8820808, Santiago, ChileCentro de Investigación Veterinaria Tandil, CONICET-CIC, Facultad de Ciencias Veterinarias, UNCPBA, Tandil 7000, Buenos Aires, ArgentinaInstituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Macul 7830490, Santiago, ChileInstituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Macul 7830490, Santiago, ChileJoint Institute for Nutrition and Food Safety, University of Maryland, College Park, MD 20742, USACenter for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD 20740, USAInstituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Macul 7830490, Santiago, ChileCattle are the main reservoir of Shiga toxin-producing <i>Escherichia coli</i> (STEC), one of the world’s most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces from central and southern Chile. We analyzed 446 cattle fecal samples and isolated non-O157 STEC from 12.6% (56/446); a total of 93 different isolates were recovered. Most isolates displayed β-glucuronidase activity (96.8%; 90/93) and fermented sorbitol (86.0%; 80/93), whereas only 39.8% (37/93) were resistant to tellurite. A subgroup of 30 representative non-O157 STEC isolates was selected for whole-genome sequencing and bioinformatics analysis. In silico analysis showed that they grouped into 16 different sequence types and 17 serotypes; the serotypes most frequently identified were O116:H21 and O168:H8 (13% each). A single isolate of serotype O26:H11 was recovered. One isolate was resistant to tetracycline and carried resistance genes <i>tet(A)</i> and <i>tet(R)</i>; no other isolate displayed antimicrobial resistance or carried antimicrobial resistance genes. The intimin gene (<i>eae</i>) was identified in 13.3% (4/30) of the genomes and 90% (27/30) carried the <i>stx2</i> gene. A phylogenetic reconstruction demonstrated that the isolates clustered based on serotypes, independent of geographical origin. These results indicate that cattle in Chile carry a wide diversity of STEC potentially pathogenic for humans based on the presence of critical virulence genes.https://www.mdpi.com/2076-2615/11/8/2388STECnon-O157cattle<i>stx</i> genesvirulence genes |
| spellingShingle | Leonela Díaz Sebastian Gutierrez Andrea I Moreno-Switt Luis Pablo Hervé Christopher Hamilton-West Nora Lía Padola Paola Navarrete Angélica Reyes-Jara Jianghong Meng Narjol González-Escalona Magaly Toro Diversity of Non-O157 Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Cattle from Central and Southern Chile Animals STEC non-O157 cattle <i>stx</i> genes virulence genes |
| title | Diversity of Non-O157 Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Cattle from Central and Southern Chile |
| title_full | Diversity of Non-O157 Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Cattle from Central and Southern Chile |
| title_fullStr | Diversity of Non-O157 Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Cattle from Central and Southern Chile |
| title_full_unstemmed | Diversity of Non-O157 Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Cattle from Central and Southern Chile |
| title_short | Diversity of Non-O157 Shiga Toxin-Producing <i>Escherichia coli</i> Isolated from Cattle from Central and Southern Chile |
| title_sort | diversity of non o157 shiga toxin producing i escherichia coli i isolated from cattle from central and southern chile |
| topic | STEC non-O157 cattle <i>stx</i> genes virulence genes |
| url | https://www.mdpi.com/2076-2615/11/8/2388 |
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