Biophysical methods in early drug discovery
Biophysical methods such as mass spectrometry, surface plasmon resonance, nuclear magnetic resonance, and both differential scanning isothermal titration calorimetry are now well established as key components of the early drug discovery process. These approaches are used successfully for a range of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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International Association of Physical Chemists (IAPC)
2019-12-01
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| Series: | ADMET and DMPK |
| Subjects: | |
| Online Access: | http://pub.iapchem.org/ojs/index.php/admet/article/view/733 |
| _version_ | 1811327885919649792 |
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| author | Geoffrey Holdgate Kevin Embrey Alexander Milbradt Gareth Davies |
| author_facet | Geoffrey Holdgate Kevin Embrey Alexander Milbradt Gareth Davies |
| author_sort | Geoffrey Holdgate |
| collection | DOAJ |
| description | Biophysical methods such as mass spectrometry, surface plasmon resonance, nuclear magnetic resonance, and both differential scanning isothermal titration calorimetry are now well established as key components of the early drug discovery process. These approaches are used successfully for a range of activities, including assay development, primary screening, hit confirmation and detailed mechanistic characterisation of compound binding. Matching the speed, sensitivity and information content of the various techniques to the generation of critical data and information required at each phase of the drug discovery process has been key. This review describes the framework by which these methods have been applied in the drug discovery process and provides case studies to exemplify the impact. |
| first_indexed | 2024-04-13T15:15:48Z |
| format | Article |
| id | doaj.art-890ecf9ee33d441aa6e596338e21e865 |
| institution | Directory Open Access Journal |
| issn | 1848-7718 |
| language | English |
| last_indexed | 2024-04-13T15:15:48Z |
| publishDate | 2019-12-01 |
| publisher | International Association of Physical Chemists (IAPC) |
| record_format | Article |
| series | ADMET and DMPK |
| spelling | doaj.art-890ecf9ee33d441aa6e596338e21e8652022-12-22T02:41:52ZengInternational Association of Physical Chemists (IAPC)ADMET and DMPK1848-77182019-12-017422224110.5599/admet.733398Biophysical methods in early drug discoveryGeoffrey Holdgate0Kevin EmbreyAlexander MilbradtGareth DaviesAstraZenecaBiophysical methods such as mass spectrometry, surface plasmon resonance, nuclear magnetic resonance, and both differential scanning isothermal titration calorimetry are now well established as key components of the early drug discovery process. These approaches are used successfully for a range of activities, including assay development, primary screening, hit confirmation and detailed mechanistic characterisation of compound binding. Matching the speed, sensitivity and information content of the various techniques to the generation of critical data and information required at each phase of the drug discovery process has been key. This review describes the framework by which these methods have been applied in the drug discovery process and provides case studies to exemplify the impact.http://pub.iapchem.org/ojs/index.php/admet/article/view/733affinity selection mass spectrometrysurface plasmon resonancenuclear magnetic resonancedifferential scanning fluorimetryisothermal titration calorimetry |
| spellingShingle | Geoffrey Holdgate Kevin Embrey Alexander Milbradt Gareth Davies Biophysical methods in early drug discovery ADMET and DMPK affinity selection mass spectrometry surface plasmon resonance nuclear magnetic resonance differential scanning fluorimetry isothermal titration calorimetry |
| title | Biophysical methods in early drug discovery |
| title_full | Biophysical methods in early drug discovery |
| title_fullStr | Biophysical methods in early drug discovery |
| title_full_unstemmed | Biophysical methods in early drug discovery |
| title_short | Biophysical methods in early drug discovery |
| title_sort | biophysical methods in early drug discovery |
| topic | affinity selection mass spectrometry surface plasmon resonance nuclear magnetic resonance differential scanning fluorimetry isothermal titration calorimetry |
| url | http://pub.iapchem.org/ojs/index.php/admet/article/view/733 |
| work_keys_str_mv | AT geoffreyholdgate biophysicalmethodsinearlydrugdiscovery AT kevinembrey biophysicalmethodsinearlydrugdiscovery AT alexandermilbradt biophysicalmethodsinearlydrugdiscovery AT garethdavies biophysicalmethodsinearlydrugdiscovery |