Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress
Flooding impairs wheat growth and considerably affects yield productivity worldwide. On the other hand, irradiation with millimeter waves enhanced the growth of chickpea and soybean under flooding stress. In the current work, millimeter-wave irradiation notably enhanced wheat growth, even under floo...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/18/10360 |
| _version_ | 1797487451678179328 |
|---|---|
| author | Setsuko Komatsu Yoshie Tsutsui Takashi Furuya Hisateru Yamaguchi Keisuke Hitachi Kunihiro Tsuchida Masahiko Tani |
| author_facet | Setsuko Komatsu Yoshie Tsutsui Takashi Furuya Hisateru Yamaguchi Keisuke Hitachi Kunihiro Tsuchida Masahiko Tani |
| author_sort | Setsuko Komatsu |
| collection | DOAJ |
| description | Flooding impairs wheat growth and considerably affects yield productivity worldwide. On the other hand, irradiation with millimeter waves enhanced the growth of chickpea and soybean under flooding stress. In the current work, millimeter-wave irradiation notably enhanced wheat growth, even under flooding stress. To explore the protective mechanisms of millimeter-wave irradiation on wheat under flooding, quantitative proteomics was performed. According to functional categorization, proteins whose abundances were changed significantly with and without irradiation under flooding stress were correlated to glycolysis, reactive-oxygen species scavenging, cell organization, and hormonal metabolism. Immunoblot analysis confirmed that fructose-bisphosphate aldolase and β tubulin accumulated in root and leaf under flooding; however, even in such condition, their accumulations were recovered to the control level in irradiated wheat. The abundance of ascorbate peroxidase increased in leaf under flooding and recovered to the control level in irradiated wheat. Because the abundance of auxin-related proteins changed with millimeter-wave irradiation, auxin was applied to wheat under flooding, resulting in the application of auxin improving its growth, even in such condition. These results suggest that millimeter-wave irradiation on wheat seeds improves the recovery of plant growth from flooding via the regulation of glycolysis, reactive-oxygen species scavenging, and cell organization. Additionally, millimeter-wave irradiation could promote tolerance against flooding through the regulation of auxin contents in wheat. |
| first_indexed | 2024-03-09T23:47:50Z |
| format | Article |
| id | doaj.art-89125c5854c24176bba4648c05826f7c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:47:50Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-89125c5854c24176bba4648c05826f7c2023-11-23T16:40:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181036010.3390/ijms231810360Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding StressSetsuko Komatsu0Yoshie Tsutsui1Takashi Furuya2Hisateru Yamaguchi3Keisuke Hitachi4Kunihiro Tsuchida5Masahiko Tani6Faculty of Environment and Information Sciences, Fukui University of Technology, Fukui 910-8505, JapanFaculty of Environment and Information Sciences, Fukui University of Technology, Fukui 910-8505, JapanResearch Center for Development of Far-Infrared Region, University of Fukui, Fukui 910-8507, JapanDepartment of Medical Technology, Yokkaichi Nursing and Medical Care University, Yokkaichi 512-8045, JapanInstitute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, JapanInstitute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, JapanResearch Center for Development of Far-Infrared Region, University of Fukui, Fukui 910-8507, JapanFlooding impairs wheat growth and considerably affects yield productivity worldwide. On the other hand, irradiation with millimeter waves enhanced the growth of chickpea and soybean under flooding stress. In the current work, millimeter-wave irradiation notably enhanced wheat growth, even under flooding stress. To explore the protective mechanisms of millimeter-wave irradiation on wheat under flooding, quantitative proteomics was performed. According to functional categorization, proteins whose abundances were changed significantly with and without irradiation under flooding stress were correlated to glycolysis, reactive-oxygen species scavenging, cell organization, and hormonal metabolism. Immunoblot analysis confirmed that fructose-bisphosphate aldolase and β tubulin accumulated in root and leaf under flooding; however, even in such condition, their accumulations were recovered to the control level in irradiated wheat. The abundance of ascorbate peroxidase increased in leaf under flooding and recovered to the control level in irradiated wheat. Because the abundance of auxin-related proteins changed with millimeter-wave irradiation, auxin was applied to wheat under flooding, resulting in the application of auxin improving its growth, even in such condition. These results suggest that millimeter-wave irradiation on wheat seeds improves the recovery of plant growth from flooding via the regulation of glycolysis, reactive-oxygen species scavenging, and cell organization. Additionally, millimeter-wave irradiation could promote tolerance against flooding through the regulation of auxin contents in wheat.https://www.mdpi.com/1422-0067/23/18/10360proteomicswheatfloodingmillimeter-wave irradiation |
| spellingShingle | Setsuko Komatsu Yoshie Tsutsui Takashi Furuya Hisateru Yamaguchi Keisuke Hitachi Kunihiro Tsuchida Masahiko Tani Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress International Journal of Molecular Sciences proteomics wheat flooding millimeter-wave irradiation |
| title | Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress |
| title_full | Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress |
| title_fullStr | Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress |
| title_full_unstemmed | Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress |
| title_short | Proteomic and Biochemical Approaches Elucidate the Role of Millimeter-Wave Irradiation in Wheat Growth under Flooding Stress |
| title_sort | proteomic and biochemical approaches elucidate the role of millimeter wave irradiation in wheat growth under flooding stress |
| topic | proteomics wheat flooding millimeter-wave irradiation |
| url | https://www.mdpi.com/1422-0067/23/18/10360 |
| work_keys_str_mv | AT setsukokomatsu proteomicandbiochemicalapproacheselucidatetheroleofmillimeterwaveirradiationinwheatgrowthunderfloodingstress AT yoshietsutsui proteomicandbiochemicalapproacheselucidatetheroleofmillimeterwaveirradiationinwheatgrowthunderfloodingstress AT takashifuruya proteomicandbiochemicalapproacheselucidatetheroleofmillimeterwaveirradiationinwheatgrowthunderfloodingstress AT hisateruyamaguchi proteomicandbiochemicalapproacheselucidatetheroleofmillimeterwaveirradiationinwheatgrowthunderfloodingstress AT keisukehitachi proteomicandbiochemicalapproacheselucidatetheroleofmillimeterwaveirradiationinwheatgrowthunderfloodingstress AT kunihirotsuchida proteomicandbiochemicalapproacheselucidatetheroleofmillimeterwaveirradiationinwheatgrowthunderfloodingstress AT masahikotani proteomicandbiochemicalapproacheselucidatetheroleofmillimeterwaveirradiationinwheatgrowthunderfloodingstress |