Three-tiered authentication of herbal traditional Chinese medicine ingredients used in women’s health provides progressive qualitative and quantitative insight
Traditional Chinese Medicine (TCM) herbal products are increasingly used in Europe, but prevalent authentication methods have significant gaps in detection. In this study, three authentication methods were tested in a tiered approach to improve accuracy on a collection of 51 TCM plant ingredients ob...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1353434/full |
| _version_ | 1797324409408585728 |
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| author | Felicitas Mück Francesca Scotti Quentin Mauvisseau Birgitte Lisbeth Graae Thorbek Helle Wangensteen Hugo J. de Boer |
| author_facet | Felicitas Mück Francesca Scotti Quentin Mauvisseau Birgitte Lisbeth Graae Thorbek Helle Wangensteen Hugo J. de Boer |
| author_sort | Felicitas Mück |
| collection | DOAJ |
| description | Traditional Chinese Medicine (TCM) herbal products are increasingly used in Europe, but prevalent authentication methods have significant gaps in detection. In this study, three authentication methods were tested in a tiered approach to improve accuracy on a collection of 51 TCM plant ingredients obtained on the European market. We show the relative performance of conventional barcoding, metabarcoding and standardized chromatographic profiling for TCM ingredients used in one of the most diagnosed disease patterns in women, endometriosis. DNA barcoding using marker ITS2 and chromatographic profiling are methods of choice reported by regulatory authorities and relevant national pharmacopeias. HPTLC was shown to be a valuable authentication tool, combined with metabarcoding, which gives an increased resolution on species diversity, despite dealing with highly processed herbal ingredients. Conventional DNA barcoding as a recommended method was shown to be an insufficient tool for authentication of these samples, while DNA metabarcoding yields an insight into biological contaminants. We conclude that a tiered identification strategy can provide progressive qualitative and quantitative insight in an integrative approach for quality control of processed herbal ingredients. |
| first_indexed | 2024-03-08T05:55:44Z |
| format | Article |
| id | doaj.art-8914d59f318241049fa58bb6e957b84a |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-08T05:55:44Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-8914d59f318241049fa58bb6e957b84a2024-02-05T04:30:54ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-02-011510.3389/fphar.2024.13534341353434Three-tiered authentication of herbal traditional Chinese medicine ingredients used in women’s health provides progressive qualitative and quantitative insightFelicitas Mück0Francesca Scotti1Quentin Mauvisseau2Birgitte Lisbeth Graae Thorbek3Helle Wangensteen4Hugo J. de Boer5Section for Pharmaceutical Chemistry, Department of Pharmacy, University of Oslo, Oslo, NorwayDepartment of Pharmaceutical and Biological Chemistry, School of Pharmacy, University College London, London, United KingdomNatural History Museum, University of Oslo, Oslo, NorwayNatural History Museum, University of Oslo, Oslo, NorwaySection for Pharmaceutical Chemistry, Department of Pharmacy, University of Oslo, Oslo, NorwayNatural History Museum, University of Oslo, Oslo, NorwayTraditional Chinese Medicine (TCM) herbal products are increasingly used in Europe, but prevalent authentication methods have significant gaps in detection. In this study, three authentication methods were tested in a tiered approach to improve accuracy on a collection of 51 TCM plant ingredients obtained on the European market. We show the relative performance of conventional barcoding, metabarcoding and standardized chromatographic profiling for TCM ingredients used in one of the most diagnosed disease patterns in women, endometriosis. DNA barcoding using marker ITS2 and chromatographic profiling are methods of choice reported by regulatory authorities and relevant national pharmacopeias. HPTLC was shown to be a valuable authentication tool, combined with metabarcoding, which gives an increased resolution on species diversity, despite dealing with highly processed herbal ingredients. Conventional DNA barcoding as a recommended method was shown to be an insufficient tool for authentication of these samples, while DNA metabarcoding yields an insight into biological contaminants. We conclude that a tiered identification strategy can provide progressive qualitative and quantitative insight in an integrative approach for quality control of processed herbal ingredients.https://www.frontiersin.org/articles/10.3389/fphar.2024.1353434/fullchemical fingerprintingDNA barcodingendometriosispharmacovigilanceTraditional Chinese Medicinewomen’s healthcare |
| spellingShingle | Felicitas Mück Francesca Scotti Quentin Mauvisseau Birgitte Lisbeth Graae Thorbek Helle Wangensteen Hugo J. de Boer Three-tiered authentication of herbal traditional Chinese medicine ingredients used in women’s health provides progressive qualitative and quantitative insight Frontiers in Pharmacology chemical fingerprinting DNA barcoding endometriosis pharmacovigilance Traditional Chinese Medicine women’s healthcare |
| title | Three-tiered authentication of herbal traditional Chinese medicine ingredients used in women’s health provides progressive qualitative and quantitative insight |
| title_full | Three-tiered authentication of herbal traditional Chinese medicine ingredients used in women’s health provides progressive qualitative and quantitative insight |
| title_fullStr | Three-tiered authentication of herbal traditional Chinese medicine ingredients used in women’s health provides progressive qualitative and quantitative insight |
| title_full_unstemmed | Three-tiered authentication of herbal traditional Chinese medicine ingredients used in women’s health provides progressive qualitative and quantitative insight |
| title_short | Three-tiered authentication of herbal traditional Chinese medicine ingredients used in women’s health provides progressive qualitative and quantitative insight |
| title_sort | three tiered authentication of herbal traditional chinese medicine ingredients used in women s health provides progressive qualitative and quantitative insight |
| topic | chemical fingerprinting DNA barcoding endometriosis pharmacovigilance Traditional Chinese Medicine women’s healthcare |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1353434/full |
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