Characterization and in vivo evaluation of a fabricated absorbable poly(vinyl alcohol)-based hernia mesh
The most widely taken medical approach toward hernia repair involves the implementation of a prosthetic mesh to cover the herniated site and reinforce the weakened area of the abdominal wall. Biodegradable meshes can serve as biocompatible grafts with a low risk of infection. However, their major co...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023094872 |
| _version_ | 1797429699643703296 |
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| author | Erfan Dorkhani Bahareh Darzi Laleh Foroutani Zahra Ebrahim Soltani Seyed Mohsen Ahmadi Tafti |
| author_facet | Erfan Dorkhani Bahareh Darzi Laleh Foroutani Zahra Ebrahim Soltani Seyed Mohsen Ahmadi Tafti |
| author_sort | Erfan Dorkhani |
| collection | DOAJ |
| description | The most widely taken medical approach toward hernia repair involves the implementation of a prosthetic mesh to cover the herniated site and reinforce the weakened area of the abdominal wall. Biodegradable meshes can serve as biocompatible grafts with a low risk of infection. However, their major complication is associated with a high rate of degradation and hernia recurrence. We proposed a facile and cost-effective method to fabricate a poly(vinyl alcohol)-based mesh, using the solution casting technique. The inclusion of zinc oxide nanoparticles, citric acid, and three cycles of freeze-thaw were intended to ameliorate the mechanical properties of poly(vinyl alcohol). Several characterization, cell culture, and animal studies were conducted. Swelling and water contact angle measurements confirmed good water uptake capacity and wetting behavior of the final mesh sample. The synthesized mesh acquired a high mechanical strength of 52.8 MPa, and its weight loss was decreased to 39 %. No cytotoxicity was found in all samples. In vivo experiments revealed that less adhesion and granuloma formation, greater tissue integration, and notably higher neovascularization rate were resulted from implanting this fabricated hernia mesh, compared to commercial Prolene® mesh. Furthermore, the amount of collagen deposition and influential growth factors were enhanced when rats were treated with the proposed mesh instead of Prolene®. |
| first_indexed | 2024-03-09T09:16:56Z |
| format | Article |
| id | doaj.art-891c4541c75343c199d17e69db8de241 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:16:56Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-891c4541c75343c199d17e69db8de2412023-12-02T07:05:38ZengElsevierHeliyon2405-84402023-11-01911e22279Characterization and in vivo evaluation of a fabricated absorbable poly(vinyl alcohol)-based hernia meshErfan Dorkhani0Bahareh Darzi1Laleh Foroutani2Zahra Ebrahim Soltani3Seyed Mohsen Ahmadi Tafti4Research Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran 1411713138, Iran; School of Chemical Engineering, College of Engineering, University of Tehran, Tehran 1417614411, IranResearch Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran 1411713138, Iran; School of Chemical Engineering, College of Engineering, University of Tehran, Tehran 1417614411, IranResearch Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran 1411713138, Iran; Colorectal Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran 1419733141, IranExperimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, IranResearch Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran 1411713138, Iran; Colorectal Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran 1419733141, Iran; Corresponding author. Colorectal Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran 1419733141, Iran.The most widely taken medical approach toward hernia repair involves the implementation of a prosthetic mesh to cover the herniated site and reinforce the weakened area of the abdominal wall. Biodegradable meshes can serve as biocompatible grafts with a low risk of infection. However, their major complication is associated with a high rate of degradation and hernia recurrence. We proposed a facile and cost-effective method to fabricate a poly(vinyl alcohol)-based mesh, using the solution casting technique. The inclusion of zinc oxide nanoparticles, citric acid, and three cycles of freeze-thaw were intended to ameliorate the mechanical properties of poly(vinyl alcohol). Several characterization, cell culture, and animal studies were conducted. Swelling and water contact angle measurements confirmed good water uptake capacity and wetting behavior of the final mesh sample. The synthesized mesh acquired a high mechanical strength of 52.8 MPa, and its weight loss was decreased to 39 %. No cytotoxicity was found in all samples. In vivo experiments revealed that less adhesion and granuloma formation, greater tissue integration, and notably higher neovascularization rate were resulted from implanting this fabricated hernia mesh, compared to commercial Prolene® mesh. Furthermore, the amount of collagen deposition and influential growth factors were enhanced when rats were treated with the proposed mesh instead of Prolene®.http://www.sciencedirect.com/science/article/pii/S2405844023094872Hernia repairpoly(vinyl alcohol)Solution castingZinc oxide nanoparticlesCitric acidFreeze-thaw |
| spellingShingle | Erfan Dorkhani Bahareh Darzi Laleh Foroutani Zahra Ebrahim Soltani Seyed Mohsen Ahmadi Tafti Characterization and in vivo evaluation of a fabricated absorbable poly(vinyl alcohol)-based hernia mesh Heliyon Hernia repair poly(vinyl alcohol) Solution casting Zinc oxide nanoparticles Citric acid Freeze-thaw |
| title | Characterization and in vivo evaluation of a fabricated absorbable poly(vinyl alcohol)-based hernia mesh |
| title_full | Characterization and in vivo evaluation of a fabricated absorbable poly(vinyl alcohol)-based hernia mesh |
| title_fullStr | Characterization and in vivo evaluation of a fabricated absorbable poly(vinyl alcohol)-based hernia mesh |
| title_full_unstemmed | Characterization and in vivo evaluation of a fabricated absorbable poly(vinyl alcohol)-based hernia mesh |
| title_short | Characterization and in vivo evaluation of a fabricated absorbable poly(vinyl alcohol)-based hernia mesh |
| title_sort | characterization and in vivo evaluation of a fabricated absorbable poly vinyl alcohol based hernia mesh |
| topic | Hernia repair poly(vinyl alcohol) Solution casting Zinc oxide nanoparticles Citric acid Freeze-thaw |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023094872 |
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