Molecular typing of clostridium perfringens toxins (α, β, ε, ι) and type "a" multidrug resistance profile in diarrheic goats in pakistan
Clostridium perfringens (C. perfringens) causes disease, generally, named as enterotoxemia in the animals. This bacterium is a normal inhabitant in gastro-intestinal tract (GIT) of animals and become harmful by increasing its colony counts as well as toxin liberation whenever gets an opportunity of...
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| Format: | Article |
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Kafkas University, Faculty of Veterinary Medicine
2017-12-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2316 |
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| author | Kashif HUSSAIN Muhammad IJAZ Aneela Zameer DURRANI Aftab Ahmad ANJUM Shahid Hussain FAROOQI Amjad Islam AQIB Abdullah Saghir AHMAD |
| author_facet | Kashif HUSSAIN Muhammad IJAZ Aneela Zameer DURRANI Aftab Ahmad ANJUM Shahid Hussain FAROOQI Amjad Islam AQIB Abdullah Saghir AHMAD |
| author_sort | Kashif HUSSAIN |
| collection | DOAJ |
| description | Clostridium perfringens (C. perfringens) causes disease, generally, named as enterotoxemia in the animals. This bacterium is a normal
inhabitant in gastro-intestinal tract (GIT) of animals and become harmful by increasing its colony counts as well as toxin liberation whenever
gets an opportunity of favorable conditions. This study focused molecular typing of C. perfringens (α, β, ε, ι toxins) and type "A" multidrug
resistance profile in diarrheic goats in Pakistan. Diarrheic fecal samples (n=192) were collected from goats and 80.73% (155/192) of the
samples were found positive for C. perfringens on the basis of culture growth and PCR. Elevated C. perfringens counts (>107 CFU/g) were
recorded in 33.55% (52/155) of positive samples, while, 66.45% (103/155) of the positive sample appeared in normal range of bacterial
counts (104-107 CFU/g). Molecular detection was carried out by targeting specific toxin genes i.e. cpa (α), cpb (β), etx (ε) and iap (ι) of C.
perfringens using PCR. Among the positive samples, 78.06% of the isolates were found as type "A", 5.16% isolates were type "B", 3.23% isolates
were type "C" while, 13.55% of the positive samples were type "D" C. perfringens. None of the isolates was found positive for iap toxin gene
(type "E"). C. perfringens type "A" was subjected to in-vitro antibiotic sensitivity test. Penicillin, ciprofloxacin and ceftriaxone were found
sensitive while bacitracin, amoxicillin and ampicillin were found least sensitive antibiotics. This study concludes that C. perfringens type "A"
is highly prevalent among goats in Pakistan and clinical cases of enterotoxemia can be effectively dealt with penicillin, ciprofloxacin and
ceftriaxone antibiotics. |
| first_indexed | 2024-03-13T04:37:29Z |
| format | Article |
| id | doaj.art-89247fc2682e4c5ba446db206009596b |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:29Z |
| publishDate | 2017-12-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-89247fc2682e4c5ba446db206009596b2023-06-19T06:44:09ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512017-12-0124225125510.9775/kvfd.2017.187742316Molecular typing of clostridium perfringens toxins (α, β, ε, ι) and type "a" multidrug resistance profile in diarrheic goats in pakistanKashif HUSSAIN0Muhammad IJAZ1Aneela Zameer DURRANI2Aftab Ahmad ANJUM3Shahid Hussain FAROOQI4Amjad Islam AQIB5Abdullah Saghir AHMAD6Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore - PAKISTANDepartment of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore - PAKISTANDepartment of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore - PAKISTANDepartment of Microbiology, University of Veterinary and Animal Sciences, Lahore - PAKISTANDepartment of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore - PAKISTANDepartment of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore - PAKISTANDepartment of Parasitology, University of Veterinary and Animal Sciences, Lahore – PAKISTANClostridium perfringens (C. perfringens) causes disease, generally, named as enterotoxemia in the animals. This bacterium is a normal inhabitant in gastro-intestinal tract (GIT) of animals and become harmful by increasing its colony counts as well as toxin liberation whenever gets an opportunity of favorable conditions. This study focused molecular typing of C. perfringens (α, β, ε, ι toxins) and type "A" multidrug resistance profile in diarrheic goats in Pakistan. Diarrheic fecal samples (n=192) were collected from goats and 80.73% (155/192) of the samples were found positive for C. perfringens on the basis of culture growth and PCR. Elevated C. perfringens counts (>107 CFU/g) were recorded in 33.55% (52/155) of positive samples, while, 66.45% (103/155) of the positive sample appeared in normal range of bacterial counts (104-107 CFU/g). Molecular detection was carried out by targeting specific toxin genes i.e. cpa (α), cpb (β), etx (ε) and iap (ι) of C. perfringens using PCR. Among the positive samples, 78.06% of the isolates were found as type "A", 5.16% isolates were type "B", 3.23% isolates were type "C" while, 13.55% of the positive samples were type "D" C. perfringens. None of the isolates was found positive for iap toxin gene (type "E"). C. perfringens type "A" was subjected to in-vitro antibiotic sensitivity test. Penicillin, ciprofloxacin and ceftriaxone were found sensitive while bacitracin, amoxicillin and ampicillin were found least sensitive antibiotics. This study concludes that C. perfringens type "A" is highly prevalent among goats in Pakistan and clinical cases of enterotoxemia can be effectively dealt with penicillin, ciprofloxacin and ceftriaxone antibiotics.https://vetdergikafkas.org/pdf.php?id=2316clostridium perfringenstoxino-typingantibiotic sensitivitygoat |
| spellingShingle | Kashif HUSSAIN Muhammad IJAZ Aneela Zameer DURRANI Aftab Ahmad ANJUM Shahid Hussain FAROOQI Amjad Islam AQIB Abdullah Saghir AHMAD Molecular typing of clostridium perfringens toxins (α, β, ε, ι) and type "a" multidrug resistance profile in diarrheic goats in pakistan Kafkas Universitesi Veteriner Fakültesi Dergisi clostridium perfringens toxino-typing antibiotic sensitivity goat |
| title | Molecular typing of clostridium perfringens toxins (α, β, ε, ι) and type "a" multidrug resistance profile in diarrheic goats in pakistan |
| title_full | Molecular typing of clostridium perfringens toxins (α, β, ε, ι) and type "a" multidrug resistance profile in diarrheic goats in pakistan |
| title_fullStr | Molecular typing of clostridium perfringens toxins (α, β, ε, ι) and type "a" multidrug resistance profile in diarrheic goats in pakistan |
| title_full_unstemmed | Molecular typing of clostridium perfringens toxins (α, β, ε, ι) and type "a" multidrug resistance profile in diarrheic goats in pakistan |
| title_short | Molecular typing of clostridium perfringens toxins (α, β, ε, ι) and type "a" multidrug resistance profile in diarrheic goats in pakistan |
| title_sort | molecular typing of clostridium perfringens toxins α β ε ι and type a multidrug resistance profile in diarrheic goats in pakistan |
| topic | clostridium perfringens toxino-typing antibiotic sensitivity goat |
| url | https://vetdergikafkas.org/pdf.php?id=2316 |
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