Renal Protection of In Vivo Administration of Tempol in Streptozotocin-Induced Diabetic Rats
The present study was carried out to investigate the protective effects of tempol on renal function and the underlying mechanism in streptozotocin-induced diabetic rats. The diabetic rats were randomly divided into the model group (without tempol) and tempol group (1 mM tempol in drinking water for...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319305092 |
| _version_ | 1818511626606739456 |
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| author | Jiajie Luan Weiping Li Jia Han Wen Zhang Huiling Gong Rong Ma |
| author_facet | Jiajie Luan Weiping Li Jia Han Wen Zhang Huiling Gong Rong Ma |
| author_sort | Jiajie Luan |
| collection | DOAJ |
| description | The present study was carried out to investigate the protective effects of tempol on renal function and the underlying mechanism in streptozotocin-induced diabetic rats. The diabetic rats were randomly divided into the model group (without tempol) and tempol group (1 mM tempol in drinking water for 6 weeks). Nondiabetic rats were served as the Control group. The mRNA expression of canonical transient receptor potential 6 (TRPC6), transforming growth factor (TGF)-β1, and type IV collagen (Col IV) were examined. The malondialdehyde (MDA) level, activities of superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in renal tissues were measured to assess redox status in kidneys. We found that tempol significantly reduced 24-h urine output and urine albuminuria excretion in the diabetic rats. Compared with the model group, the concentration of MDA was significantly lower in the tempol group. In addition, diabetes decreased activities of SOD and GSH-Px and these responses were prevented by tempol treatment. Moreover, in diabetic rats, the mRNA expression levels of TGF-β1 and Col IV were upregulated. TRPC6 mRNA expression level was down-regulated in diabetic kidneys. However, all of these diabetic effects were significantly suppressed by tempol treatment. These results suggest that chronic treatment of diabetic rats with tempol can protect kidneys, possibly by reducing expression of TGF-β1, Col IV, and upregulating TRPC6 expression level. Keywords:: diabetic nephropathy, tempol, transient receptor potential 6 (TRPC6), transforming growth factor-β1, collagen IV |
| first_indexed | 2024-12-10T23:35:38Z |
| format | Article |
| id | doaj.art-892568e5af3041e4ad751e08c65b4728 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-10T23:35:38Z |
| publishDate | 2012-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-892568e5af3041e4ad751e08c65b47282022-12-22T01:29:12ZengElsevierJournal of Pharmacological Sciences1347-86132012-01-011192167176Renal Protection of In Vivo Administration of Tempol in Streptozotocin-Induced Diabetic RatsJiajie Luan0Weiping Li1Jia Han2Wen Zhang3Huiling Gong4Rong Ma5Department of Pharmacology, Anhui Medical University, Hefei, Anhui 230032, China; Department of Pharmacy, Yijishan Hospital of Wannan Medical University, Wuhu, Anhui 241001, ChinaDepartment of Pharmacology, Anhui Medical University, Hefei, Anhui 230032, China; Department of Pharmacology, Anqing Medical and Pharmaceutical College, Anqing, Anhui 246000, China; Corresponding authors. *awei_pingli@126.com, *brong.ma@unthsc.edu on May 22, 2012 (in advance)Department of Pharmacology, Anhui Medical University, Hefei, Anhui 230032, ChinaDepartment of Pharmacy, Yijishan Hospital of Wannan Medical University, Wuhu, Anhui 241001, ChinaDepartment of Pharmacology, Anhui Medical University, Hefei, Anhui 230032, ChinaDepartment of Integrative Physiology, University of North Texas Health Science Center, Fort Worth, Texas 76107, USA; Corresponding authors. *awei_pingli@126.com, *brong.ma@unthsc.edu on May 22, 2012 (in advance)The present study was carried out to investigate the protective effects of tempol on renal function and the underlying mechanism in streptozotocin-induced diabetic rats. The diabetic rats were randomly divided into the model group (without tempol) and tempol group (1 mM tempol in drinking water for 6 weeks). Nondiabetic rats were served as the Control group. The mRNA expression of canonical transient receptor potential 6 (TRPC6), transforming growth factor (TGF)-β1, and type IV collagen (Col IV) were examined. The malondialdehyde (MDA) level, activities of superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in renal tissues were measured to assess redox status in kidneys. We found that tempol significantly reduced 24-h urine output and urine albuminuria excretion in the diabetic rats. Compared with the model group, the concentration of MDA was significantly lower in the tempol group. In addition, diabetes decreased activities of SOD and GSH-Px and these responses were prevented by tempol treatment. Moreover, in diabetic rats, the mRNA expression levels of TGF-β1 and Col IV were upregulated. TRPC6 mRNA expression level was down-regulated in diabetic kidneys. However, all of these diabetic effects were significantly suppressed by tempol treatment. These results suggest that chronic treatment of diabetic rats with tempol can protect kidneys, possibly by reducing expression of TGF-β1, Col IV, and upregulating TRPC6 expression level. Keywords:: diabetic nephropathy, tempol, transient receptor potential 6 (TRPC6), transforming growth factor-β1, collagen IVhttp://www.sciencedirect.com/science/article/pii/S1347861319305092 |
| spellingShingle | Jiajie Luan Weiping Li Jia Han Wen Zhang Huiling Gong Rong Ma Renal Protection of In Vivo Administration of Tempol in Streptozotocin-Induced Diabetic Rats Journal of Pharmacological Sciences |
| title | Renal Protection of In Vivo Administration of Tempol in Streptozotocin-Induced Diabetic Rats |
| title_full | Renal Protection of In Vivo Administration of Tempol in Streptozotocin-Induced Diabetic Rats |
| title_fullStr | Renal Protection of In Vivo Administration of Tempol in Streptozotocin-Induced Diabetic Rats |
| title_full_unstemmed | Renal Protection of In Vivo Administration of Tempol in Streptozotocin-Induced Diabetic Rats |
| title_short | Renal Protection of In Vivo Administration of Tempol in Streptozotocin-Induced Diabetic Rats |
| title_sort | renal protection of in vivo administration of tempol in streptozotocin induced diabetic rats |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319305092 |
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