Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.)
Polyurethane is a class of polymers characterized by their substantial tensile strength and high melting points which enables them to be extremely durable. Despite its xenobiotic origin, polyure-thane has been found susceptible to biodegradation by microorgan-isms. The main object of this research w...
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| Format: | Article |
| Language: | English |
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UIN Sunan Gunung Djati
2019-11-01
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| Series: | Jurnal Biodjati |
| Subjects: | |
| Online Access: | http://journal.uinsgd.ac.id/index.php/biodjati/article/view/4525 |
| _version_ | 1818598362500300800 |
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| author | Farah Aldila Ari Susilowati Ratna Setyaningsih |
| author_facet | Farah Aldila Ari Susilowati Ratna Setyaningsih |
| author_sort | Farah Aldila |
| collection | DOAJ |
| description | Polyurethane is a class of polymers characterized by their substantial tensile strength and high melting points which enables them to be extremely durable. Despite its xenobiotic origin, polyure-thane has been found susceptible to biodegradation by microorgan-isms. The main object of this research was to investigate polyurethane degradation by the bacterium isolated from decayed teak wood (Tec-tona grandis Linn. f.). Polyurethane degrading bacteria were isolated using selective medium in the form of LB (Luria Bertani) with the ad-dition of 0.3% Impranil. Bacterial biodegradation activities shown by the changes of polyurethane structure analyzed by FT-IR spectrosco-py. The bacterial identification was carried out based on observations on the morphological characteristics of the colonies, cell morphology and sequences of 16S rRNA encoding genes. One of the potential iso-late that successfully isolated was K9, which demonstrates the disap-pearance of the 1735/cm peak of the characteristic function urethane in the FT-IR analysis. Analysis of the 16S rRNA encoding gene showed that the potential isolate having 98% similarity index to Bacillus safen-sis strain FO-36b. The activity shown by the isolate suggests that the bacteria could be a promising agent for polyurethane degradation. |
| first_indexed | 2024-12-16T12:02:30Z |
| format | Article |
| id | doaj.art-89266cd5d783445bb8af2f908598e39a |
| institution | Directory Open Access Journal |
| issn | 2541-4208 2548-1606 |
| language | English |
| last_indexed | 2024-12-16T12:02:30Z |
| publishDate | 2019-11-01 |
| publisher | UIN Sunan Gunung Djati |
| record_format | Article |
| series | Jurnal Biodjati |
| spelling | doaj.art-89266cd5d783445bb8af2f908598e39a2022-12-21T22:32:25ZengUIN Sunan Gunung DjatiJurnal Biodjati2541-42082548-16062019-11-014222523510.15575/biodjati.v4i2.45252885Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.)Farah Aldila0Ari Susilowati1Ratna Setyaningsih2Bioscience Program, Pascasarjana, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36 A Surakarta 57126, Telp./Fax. +62-815-42096292Bioscience Program, Pascasarjana, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36 A Surakarta 57126, Telp./Fax. +62-815-42096292Bioscience Program, Pascasarjana, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36 A Surakarta 57126, Telp./Fax. +62-815-42096292Polyurethane is a class of polymers characterized by their substantial tensile strength and high melting points which enables them to be extremely durable. Despite its xenobiotic origin, polyure-thane has been found susceptible to biodegradation by microorgan-isms. The main object of this research was to investigate polyurethane degradation by the bacterium isolated from decayed teak wood (Tec-tona grandis Linn. f.). Polyurethane degrading bacteria were isolated using selective medium in the form of LB (Luria Bertani) with the ad-dition of 0.3% Impranil. Bacterial biodegradation activities shown by the changes of polyurethane structure analyzed by FT-IR spectrosco-py. The bacterial identification was carried out based on observations on the morphological characteristics of the colonies, cell morphology and sequences of 16S rRNA encoding genes. One of the potential iso-late that successfully isolated was K9, which demonstrates the disap-pearance of the 1735/cm peak of the characteristic function urethane in the FT-IR analysis. Analysis of the 16S rRNA encoding gene showed that the potential isolate having 98% similarity index to Bacillus safen-sis strain FO-36b. The activity shown by the isolate suggests that the bacteria could be a promising agent for polyurethane degradation.http://journal.uinsgd.ac.id/index.php/biodjati/article/view/4525biodegradationpolyurethaneteak wood |
| spellingShingle | Farah Aldila Ari Susilowati Ratna Setyaningsih Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.) Jurnal Biodjati biodegradation polyurethane teak wood |
| title | Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.) |
| title_full | Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.) |
| title_fullStr | Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.) |
| title_full_unstemmed | Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.) |
| title_short | Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.) |
| title_sort | polyurethane degrading bacteria isolated from decayed teak wood tectona grandis linn f |
| topic | biodegradation polyurethane teak wood |
| url | http://journal.uinsgd.ac.id/index.php/biodjati/article/view/4525 |
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