Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation
Nanodiscs belong to a category of water-soluble lipid bilayer nanoparticles. In vivo nanodisc platforms are useful for studying isolated membrane proteins in their native lipid environment. Thus, the development of a practical method for nanodisc reconstruction has garnered consider-able research in...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/6/1300 |
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| author | Tomohiro Imura Satohiro Yanagisawa Yuri Ikeda Ryodai Moriyama Kenichi Sakai Hideki Sakai Toshiaki Taira |
| author_facet | Tomohiro Imura Satohiro Yanagisawa Yuri Ikeda Ryodai Moriyama Kenichi Sakai Hideki Sakai Toshiaki Taira |
| author_sort | Tomohiro Imura |
| collection | DOAJ |
| description | Nanodiscs belong to a category of water-soluble lipid bilayer nanoparticles. In vivo nanodisc platforms are useful for studying isolated membrane proteins in their native lipid environment. Thus, the development of a practical method for nanodisc reconstruction has garnered consider-able research interest. This paper reports the self-assembly of a mixture of bio-derived cyclic peptide, surfactin (SF), and <span style="font-variant: small-caps;">l</span>-α-dimyristoylphosphatidylcholine (DMPC). We found that SF induced the solubilization of DMPC multilamellar vesicles to form their nanodiscs, which was confirmed by size-exclusion chromatography, dynamic light scattering, and transmission electron microscopy analyses. Owing to its amphiphilic nature, the self-assembled structure prevents the exposure of the hydrophobic lipid core to aqueous media, thus embedding ubiquinol (CoQ10) as a hydrophobic model compound within the inner region of the nanodiscs. These results highlight the feasibility of preparing nanodiscs without the need for laborious procedures, thereby showcasing their potential to serve as promising carriers for membrane proteins and various organic compounds. Additionally, the regulated self-assembly of the DMPC/SF mixture led to the formation of fibrous architectures. These results show the potential of this mixture to function as a nanoscale membrane surface for investigating molecular recognition events. |
| first_indexed | 2024-04-24T17:58:25Z |
| format | Article |
| id | doaj.art-8926bce3b4f140e6b9c4f30e95d45c33 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-24T17:58:25Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8926bce3b4f140e6b9c4f30e95d45c332024-03-27T13:56:59ZengMDPI AGMolecules1420-30492024-03-01296130010.3390/molecules29061300Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture FormationTomohiro Imura0Satohiro Yanagisawa1Yuri Ikeda2Ryodai Moriyama3Kenichi Sakai4Hideki Sakai5Toshiaki Taira6Research Institute for Chemical Process Technology, National Institute of Advanced Industrial Science and Technology (AIST), 4-2-1 Nigatake, Miyagino, Sendai 983-8551, JapanNew Business Development Division, Kaneka Corporation, 2-3-18, Nakanoshima, Kita-ku, Osaka 530-8288, JapanFaculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, JapanFaculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, JapanFaculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, JapanFaculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, JapanResearch Institute for Chemical Process Technology, National Institute of Advanced Industrial Science and Technology (AIST), 4-2-1 Nigatake, Miyagino, Sendai 983-8551, JapanNanodiscs belong to a category of water-soluble lipid bilayer nanoparticles. In vivo nanodisc platforms are useful for studying isolated membrane proteins in their native lipid environment. Thus, the development of a practical method for nanodisc reconstruction has garnered consider-able research interest. This paper reports the self-assembly of a mixture of bio-derived cyclic peptide, surfactin (SF), and <span style="font-variant: small-caps;">l</span>-α-dimyristoylphosphatidylcholine (DMPC). We found that SF induced the solubilization of DMPC multilamellar vesicles to form their nanodiscs, which was confirmed by size-exclusion chromatography, dynamic light scattering, and transmission electron microscopy analyses. Owing to its amphiphilic nature, the self-assembled structure prevents the exposure of the hydrophobic lipid core to aqueous media, thus embedding ubiquinol (CoQ10) as a hydrophobic model compound within the inner region of the nanodiscs. These results highlight the feasibility of preparing nanodiscs without the need for laborious procedures, thereby showcasing their potential to serve as promising carriers for membrane proteins and various organic compounds. Additionally, the regulated self-assembly of the DMPC/SF mixture led to the formation of fibrous architectures. These results show the potential of this mixture to function as a nanoscale membrane surface for investigating molecular recognition events.https://www.mdpi.com/1420-3049/29/6/1300surfactinphospholipidnanodiscsfibrous aggregates |
| spellingShingle | Tomohiro Imura Satohiro Yanagisawa Yuri Ikeda Ryodai Moriyama Kenichi Sakai Hideki Sakai Toshiaki Taira Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation Molecules surfactin phospholipid nanodiscs fibrous aggregates |
| title | Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation |
| title_full | Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation |
| title_fullStr | Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation |
| title_full_unstemmed | Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation |
| title_short | Solubilization of Phospholipid by Surfactin Leading to Lipid Nanodisc and Fibrous Architecture Formation |
| title_sort | solubilization of phospholipid by surfactin leading to lipid nanodisc and fibrous architecture formation |
| topic | surfactin phospholipid nanodiscs fibrous aggregates |
| url | https://www.mdpi.com/1420-3049/29/6/1300 |
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