Genetic screens in isogenic mammalian cell lines without single cell cloning
Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-14620-6 |
| _version_ | 1818843332735926272 |
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| author | Peter C. DeWeirdt Annabel K. Sangree Ruth E. Hanna Kendall R. Sanson Mudra Hegde Christine Strand Nicole S. Persky John G. Doench |
| author_facet | Peter C. DeWeirdt Annabel K. Sangree Ruth E. Hanna Kendall R. Sanson Mudra Hegde Christine Strand Nicole S. Persky John G. Doench |
| author_sort | Peter C. DeWeirdt |
| collection | DOAJ |
| description | Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9. |
| first_indexed | 2024-12-19T04:56:11Z |
| format | Article |
| id | doaj.art-892a16ea5f874784a2584fee9329b79a |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T04:56:11Z |
| publishDate | 2020-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-892a16ea5f874784a2584fee9329b79a2022-12-21T20:35:14ZengNature PortfolioNature Communications2041-17232020-02-0111111510.1038/s41467-020-14620-6Genetic screens in isogenic mammalian cell lines without single cell cloningPeter C. DeWeirdt0Annabel K. Sangree1Ruth E. Hanna2Kendall R. Sanson3Mudra Hegde4Christine Strand5Nicole S. Persky6John G. Doench7Genetic Perturbation Platform, Broad Institute of MIT and HarvardGenetic Perturbation Platform, Broad Institute of MIT and HarvardGenetic Perturbation Platform, Broad Institute of MIT and HarvardGenetic Perturbation Platform, Broad Institute of MIT and HarvardGenetic Perturbation Platform, Broad Institute of MIT and HarvardGenetic Perturbation Platform, Broad Institute of MIT and HarvardGenetic Perturbation Platform, Broad Institute of MIT and HarvardGenetic Perturbation Platform, Broad Institute of MIT and HarvardIsogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.https://doi.org/10.1038/s41467-020-14620-6 |
| spellingShingle | Peter C. DeWeirdt Annabel K. Sangree Ruth E. Hanna Kendall R. Sanson Mudra Hegde Christine Strand Nicole S. Persky John G. Doench Genetic screens in isogenic mammalian cell lines without single cell cloning Nature Communications |
| title | Genetic screens in isogenic mammalian cell lines without single cell cloning |
| title_full | Genetic screens in isogenic mammalian cell lines without single cell cloning |
| title_fullStr | Genetic screens in isogenic mammalian cell lines without single cell cloning |
| title_full_unstemmed | Genetic screens in isogenic mammalian cell lines without single cell cloning |
| title_short | Genetic screens in isogenic mammalian cell lines without single cell cloning |
| title_sort | genetic screens in isogenic mammalian cell lines without single cell cloning |
| url | https://doi.org/10.1038/s41467-020-14620-6 |
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