Use of S2.2/DOX Magnetic Nanoliposomes in MR Molecule Imaging and Targeted Thermochemotherapy for Breast Cancer In Vitro
Objective To prepare S2.2/DOX magnetic nanoliposomes by combining the potential benefits of MNPs in MRI and the targeted performance of nano-drugs as an innovative method for integrated diagnosis and treatment of breast cancer (BC). Methods We created a S2.2-PEG-MZF/DOX molecular probe by using a li...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-08-01
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| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/15330338231194498 |
| _version_ | 1827867306574741504 |
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| author | Yinxing Zhu MD Dazhuang Yang MD Ting Guo MD Mei Lin PhD |
| author_facet | Yinxing Zhu MD Dazhuang Yang MD Ting Guo MD Mei Lin PhD |
| author_sort | Yinxing Zhu MD |
| collection | DOAJ |
| description | Objective To prepare S2.2/DOX magnetic nanoliposomes by combining the potential benefits of MNPs in MRI and the targeted performance of nano-drugs as an innovative method for integrated diagnosis and treatment of breast cancer (BC). Methods We created a S2.2-PEG-MZF/DOX molecular probe by using a lipid material to encapsulate PEG-MZF-NPs and doxorubicin (DOX), and a S2.2 aptamer to target MUC1 to conjugate with PEG-MZF/DOX nanoliposomes. The potential of probe for cell-specific targeting and magnetic resonance (MR) molecular imaging was evaluated by MR scanner and Prussian blue staining. Additionally, we explored the feasibility by using nanoliposome magnetic induction heating to interfere with MCF-7 (MUC1+) BC cells under the influence of an alternating magnetic field (AMF). Results PEG-MZF-NPs were biologically safe. The T2 relaxation rate of PEG-MZF-NPs was found to inhibit T2 signal in a concentration-dependent manner, and the T2 signal of the S2.2-PEG-MZF molecular probe in MCF-7 cells was significantly lower than that in PEG-MZF-NPs group. Moreover, the T2 signal reduction was more pronounced in MCF-7 cells than in the hepatoma cell line HepG2 (MUC1-), suggesting a strong MRI potential of the S2.2-PEG-MZF molecular probe. The S2.2-PEG-MZF/DOX nanoliposome was able to achieve the desired temperature range for tumor hyperthermia (42-44 °C) in vitro. The S2.2-PEG-MZF/DOX nanoliposome accompanied by magnetic fluid hyperthermia (MFH) could inhibit proliferation and invasion and induce apoptosis of MCF-7 cells. The effects of this approach were significantly higher than those observed in the other groups. Conclusion We successfully developed a novel technique for BC diagnosis and treatment using thermochemotherapy under the guidance of MR molecular imaging. This approach holds great potential for improving the management of this devastating disease in the future. |
| first_indexed | 2024-03-12T15:16:07Z |
| format | Article |
| id | doaj.art-8930a1d8c75a44149102358ec6896d25 |
| institution | Directory Open Access Journal |
| issn | 1533-0338 |
| language | English |
| last_indexed | 2024-03-12T15:16:07Z |
| publishDate | 2023-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj.art-8930a1d8c75a44149102358ec6896d252023-08-11T13:33:54ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382023-08-012210.1177/15330338231194498Use of S2.2/DOX Magnetic Nanoliposomes in MR Molecule Imaging and Targeted Thermochemotherapy for Breast Cancer In VitroYinxing Zhu MD0Dazhuang Yang MD1Ting Guo MD2Mei Lin PhD3 Taizhou School of Clinical Medicine, Nanjing Medical University, The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, Jiangsu, China Imaging Department, General Hospital of Xuzhou Mining Group, Xuzhou, Jiangsu, China Institute of Clinical Medicine, The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, Jiangsu, China Clinical Laboratory, The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, Jiangsu, ChinaObjective To prepare S2.2/DOX magnetic nanoliposomes by combining the potential benefits of MNPs in MRI and the targeted performance of nano-drugs as an innovative method for integrated diagnosis and treatment of breast cancer (BC). Methods We created a S2.2-PEG-MZF/DOX molecular probe by using a lipid material to encapsulate PEG-MZF-NPs and doxorubicin (DOX), and a S2.2 aptamer to target MUC1 to conjugate with PEG-MZF/DOX nanoliposomes. The potential of probe for cell-specific targeting and magnetic resonance (MR) molecular imaging was evaluated by MR scanner and Prussian blue staining. Additionally, we explored the feasibility by using nanoliposome magnetic induction heating to interfere with MCF-7 (MUC1+) BC cells under the influence of an alternating magnetic field (AMF). Results PEG-MZF-NPs were biologically safe. The T2 relaxation rate of PEG-MZF-NPs was found to inhibit T2 signal in a concentration-dependent manner, and the T2 signal of the S2.2-PEG-MZF molecular probe in MCF-7 cells was significantly lower than that in PEG-MZF-NPs group. Moreover, the T2 signal reduction was more pronounced in MCF-7 cells than in the hepatoma cell line HepG2 (MUC1-), suggesting a strong MRI potential of the S2.2-PEG-MZF molecular probe. The S2.2-PEG-MZF/DOX nanoliposome was able to achieve the desired temperature range for tumor hyperthermia (42-44 °C) in vitro. The S2.2-PEG-MZF/DOX nanoliposome accompanied by magnetic fluid hyperthermia (MFH) could inhibit proliferation and invasion and induce apoptosis of MCF-7 cells. The effects of this approach were significantly higher than those observed in the other groups. Conclusion We successfully developed a novel technique for BC diagnosis and treatment using thermochemotherapy under the guidance of MR molecular imaging. This approach holds great potential for improving the management of this devastating disease in the future.https://doi.org/10.1177/15330338231194498 |
| spellingShingle | Yinxing Zhu MD Dazhuang Yang MD Ting Guo MD Mei Lin PhD Use of S2.2/DOX Magnetic Nanoliposomes in MR Molecule Imaging and Targeted Thermochemotherapy for Breast Cancer In Vitro Technology in Cancer Research & Treatment |
| title | Use of S2.2/DOX Magnetic Nanoliposomes
in MR Molecule Imaging and Targeted Thermochemotherapy for Breast Cancer
In Vitro |
| title_full | Use of S2.2/DOX Magnetic Nanoliposomes
in MR Molecule Imaging and Targeted Thermochemotherapy for Breast Cancer
In Vitro |
| title_fullStr | Use of S2.2/DOX Magnetic Nanoliposomes
in MR Molecule Imaging and Targeted Thermochemotherapy for Breast Cancer
In Vitro |
| title_full_unstemmed | Use of S2.2/DOX Magnetic Nanoliposomes
in MR Molecule Imaging and Targeted Thermochemotherapy for Breast Cancer
In Vitro |
| title_short | Use of S2.2/DOX Magnetic Nanoliposomes
in MR Molecule Imaging and Targeted Thermochemotherapy for Breast Cancer
In Vitro |
| title_sort | use of s2 2 dox magnetic nanoliposomes in mr molecule imaging and targeted thermochemotherapy for breast cancer in vitro |
| url | https://doi.org/10.1177/15330338231194498 |
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