Three-Dimension Resolution Enhanced Microscopy Based on Parallel Detection
Pixel reassignment image scanning microscopy (PRISM) is a useful tool to improve the resolution of confocal laser scanning microscopy (CLSM) only equipped with a detector array. However, while it can improve the lateral resolution, it has little effect on the axial resolution. Here, new microscopy h...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/11/6/2837 |
| _version_ | 1797540434737627136 |
|---|---|
| author | Zhimin Zhang Shaocong Liu Minfei He Yuran Huang Cuifang Kuang Yubing Han Xiang Hao Xu Liu |
| author_facet | Zhimin Zhang Shaocong Liu Minfei He Yuran Huang Cuifang Kuang Yubing Han Xiang Hao Xu Liu |
| author_sort | Zhimin Zhang |
| collection | DOAJ |
| description | Pixel reassignment image scanning microscopy (PRISM) is a useful tool to improve the resolution of confocal laser scanning microscopy (CLSM) only equipped with a detector array. However, while it can improve the lateral resolution, it has little effect on the axial resolution. Here, new microscopy has been proposed which combines three-dimension fluorescence emission difference microscopy (3D FED) with PRISM to further improve three-dimension resolution. We call this method three-dimension pixel reassignment fluorescence emission difference microscopy (3D-PRFED). Detailed theoretical analysis and simulation are presented in this paper. Additionally, the performance of lateral and axial resolution improvement of this method has been demonstrated by imaging 100 nm fluorescent beads and nuclear pore complexes samples. Experiment results show that this method in our system can improve lateral resolution by a factor of 1.85 and axial resolution by a factor of 1.48 compared with CLSM. |
| first_indexed | 2024-03-10T13:00:06Z |
| format | Article |
| id | doaj.art-893118b0af6e48a495d8271f5c2b2ea2 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T13:00:06Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-893118b0af6e48a495d8271f5c2b2ea22023-11-21T11:33:24ZengMDPI AGApplied Sciences2076-34172021-03-01116283710.3390/app11062837Three-Dimension Resolution Enhanced Microscopy Based on Parallel DetectionZhimin Zhang0Shaocong Liu1Minfei He2Yuran Huang3Cuifang Kuang4Yubing Han5Xiang Hao6Xu Liu7State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, ChinaPixel reassignment image scanning microscopy (PRISM) is a useful tool to improve the resolution of confocal laser scanning microscopy (CLSM) only equipped with a detector array. However, while it can improve the lateral resolution, it has little effect on the axial resolution. Here, new microscopy has been proposed which combines three-dimension fluorescence emission difference microscopy (3D FED) with PRISM to further improve three-dimension resolution. We call this method three-dimension pixel reassignment fluorescence emission difference microscopy (3D-PRFED). Detailed theoretical analysis and simulation are presented in this paper. Additionally, the performance of lateral and axial resolution improvement of this method has been demonstrated by imaging 100 nm fluorescent beads and nuclear pore complexes samples. Experiment results show that this method in our system can improve lateral resolution by a factor of 1.85 and axial resolution by a factor of 1.48 compared with CLSM.https://www.mdpi.com/2076-3417/11/6/2837spatial light modulator (SLM)detector arrayconfocal laser scanning microscopy (CLSM)pixel reassignment image scanning microscopy (PRISM)three-dimension pixel reassignment fluorescent emission different microscopy (3D-PRFED) |
| spellingShingle | Zhimin Zhang Shaocong Liu Minfei He Yuran Huang Cuifang Kuang Yubing Han Xiang Hao Xu Liu Three-Dimension Resolution Enhanced Microscopy Based on Parallel Detection Applied Sciences spatial light modulator (SLM) detector array confocal laser scanning microscopy (CLSM) pixel reassignment image scanning microscopy (PRISM) three-dimension pixel reassignment fluorescent emission different microscopy (3D-PRFED) |
| title | Three-Dimension Resolution Enhanced Microscopy Based on Parallel Detection |
| title_full | Three-Dimension Resolution Enhanced Microscopy Based on Parallel Detection |
| title_fullStr | Three-Dimension Resolution Enhanced Microscopy Based on Parallel Detection |
| title_full_unstemmed | Three-Dimension Resolution Enhanced Microscopy Based on Parallel Detection |
| title_short | Three-Dimension Resolution Enhanced Microscopy Based on Parallel Detection |
| title_sort | three dimension resolution enhanced microscopy based on parallel detection |
| topic | spatial light modulator (SLM) detector array confocal laser scanning microscopy (CLSM) pixel reassignment image scanning microscopy (PRISM) three-dimension pixel reassignment fluorescent emission different microscopy (3D-PRFED) |
| url | https://www.mdpi.com/2076-3417/11/6/2837 |
| work_keys_str_mv | AT zhiminzhang threedimensionresolutionenhancedmicroscopybasedonparalleldetection AT shaocongliu threedimensionresolutionenhancedmicroscopybasedonparalleldetection AT minfeihe threedimensionresolutionenhancedmicroscopybasedonparalleldetection AT yuranhuang threedimensionresolutionenhancedmicroscopybasedonparalleldetection AT cuifangkuang threedimensionresolutionenhancedmicroscopybasedonparalleldetection AT yubinghan threedimensionresolutionenhancedmicroscopybasedonparalleldetection AT xianghao threedimensionresolutionenhancedmicroscopybasedonparalleldetection AT xuliu threedimensionresolutionenhancedmicroscopybasedonparalleldetection |