A combination of metabolic resistance and high frequency of the 1014F kdr mutation is driving pyrethroid resistance in Anopheles coluzzii population from Guinea savanna of Cameroon
Abstract Background The scale-up in the distribution of long-lasting insecticidal nets (LLINs) and indoor residual spraying has significantly reduced malaria burden and mortality. However, insecticide resistance, among other factors, is responsible for a recent rebound in malaria transmission in 201...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3523-7 |
| _version_ | 1819106083978870784 |
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| author | Amen N. Fadel Sulaiman S. Ibrahim Magellan Tchouakui Ebai Terence Murielle J. Wondji Micareme Tchoupo Samuel Wanji Charles S. Wondji |
| author_facet | Amen N. Fadel Sulaiman S. Ibrahim Magellan Tchouakui Ebai Terence Murielle J. Wondji Micareme Tchoupo Samuel Wanji Charles S. Wondji |
| author_sort | Amen N. Fadel |
| collection | DOAJ |
| description | Abstract Background The scale-up in the distribution of long-lasting insecticidal nets (LLINs) and indoor residual spraying has significantly reduced malaria burden and mortality. However, insecticide resistance, among other factors, is responsible for a recent rebound in malaria transmission in 2015–2016, threatening the progress so far made. As a contribution towards understanding patterns of resistance and its mechanism in the field we characterized a population of Anopheles gambiae (s.l.) from Gounougou, a Guinea savanna of north/central Cameroon. Results Indoor collection conducted in September 2017 identified Anopheles coluzzii and Anopheles arabiensis as the unique Anopheles vector species, with abundances of 83 and 17%, respectively. Analysis of infection with TaqMan assays using heads/thoraces of indoor collected females of An. coluzzii revealed a low Plasmodium falciparum parasite rate of 4.7%. Bioassays conducted with female An. coluzzii revealed extreme resistance, with low mortalities of only 3.75 ± 1.25%, 3.03 ± 1.59% and 1.45 ± 1.45%, respectively, for permethrin, deltamethrin and DDT. In contrast, high susceptibility was obtained with the organophosphates and carbamates, with mortalities in the range of 98–100%. Synergist assays with piperonyl butoxide (PBO) recovered some susceptibility with increased mortality for permethrin to 14.88 ± 8.74%, and for deltamethrin to 32.50 ± 10.51% (~27-fold increase compared to mortalities with deltamethrin alone, χ 2 = 29, df = 1, P < 0.0001). These correlated with the results of cone bioassays which revealed complete loss of efficacy of Olyset®Net (0% mortality) and PermaNet®2.0 (0% mortality), and the considerable loss of efficacy of Olyset®Plus (mortality of 2 ± 2%), PermaNet®3.0 side panel (mortality of 2 ± 2%) and PermaNet3.0® roof (mortality of 16 ± 5.1%). Time-course bioassays conducted with deltamethrin established a high intensity of resistance, with LT50 of 309.09 (95% CI 253.07–393.71, Fiducial), and a resistance ratio of 93.09 compared with the fully susceptible Ngoussou laboratory colony. TaqMan genotyping revealed a high frequency of the 1014F allele (65.25%) in the An. coluzzii populations. Sequencing of a fragment of the voltage-gated sodium channel identified a single An. arabiensis female harbouring the 1014S kdr mutation. Conclusions This finding of high pyrethroid and DDT resistance in An. coluzzii from north-central Cameroon is a major obstacle to malaria control using pyrethroid bednets and indoor residual spraying with DDT. |
| first_indexed | 2024-12-22T02:32:31Z |
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| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-22T02:32:31Z |
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| series | Parasites & Vectors |
| spelling | doaj.art-89324279e3404a6094b40caef9e687ed2022-12-21T18:41:52ZengBMCParasites & Vectors1756-33052019-05-0112111310.1186/s13071-019-3523-7A combination of metabolic resistance and high frequency of the 1014F kdr mutation is driving pyrethroid resistance in Anopheles coluzzii population from Guinea savanna of CameroonAmen N. Fadel0Sulaiman S. Ibrahim1Magellan Tchouakui2Ebai Terence3Murielle J. Wondji4Micareme Tchoupo5Samuel Wanji6Charles S. Wondji7LSTM Research Unit, Centre for Research in Infectious Diseases (CRID)Vector Biology Department, Liverpool School of Tropical Medicine (LSTM)LSTM Research Unit, Centre for Research in Infectious Diseases (CRID)LSTM Research Unit, Centre for Research in Infectious Diseases (CRID)LSTM Research Unit, Centre for Research in Infectious Diseases (CRID)LSTM Research Unit, Centre for Research in Infectious Diseases (CRID)Department of Microbiology and Parasitology, University of BueaLSTM Research Unit, Centre for Research in Infectious Diseases (CRID)Abstract Background The scale-up in the distribution of long-lasting insecticidal nets (LLINs) and indoor residual spraying has significantly reduced malaria burden and mortality. However, insecticide resistance, among other factors, is responsible for a recent rebound in malaria transmission in 2015–2016, threatening the progress so far made. As a contribution towards understanding patterns of resistance and its mechanism in the field we characterized a population of Anopheles gambiae (s.l.) from Gounougou, a Guinea savanna of north/central Cameroon. Results Indoor collection conducted in September 2017 identified Anopheles coluzzii and Anopheles arabiensis as the unique Anopheles vector species, with abundances of 83 and 17%, respectively. Analysis of infection with TaqMan assays using heads/thoraces of indoor collected females of An. coluzzii revealed a low Plasmodium falciparum parasite rate of 4.7%. Bioassays conducted with female An. coluzzii revealed extreme resistance, with low mortalities of only 3.75 ± 1.25%, 3.03 ± 1.59% and 1.45 ± 1.45%, respectively, for permethrin, deltamethrin and DDT. In contrast, high susceptibility was obtained with the organophosphates and carbamates, with mortalities in the range of 98–100%. Synergist assays with piperonyl butoxide (PBO) recovered some susceptibility with increased mortality for permethrin to 14.88 ± 8.74%, and for deltamethrin to 32.50 ± 10.51% (~27-fold increase compared to mortalities with deltamethrin alone, χ 2 = 29, df = 1, P < 0.0001). These correlated with the results of cone bioassays which revealed complete loss of efficacy of Olyset®Net (0% mortality) and PermaNet®2.0 (0% mortality), and the considerable loss of efficacy of Olyset®Plus (mortality of 2 ± 2%), PermaNet®3.0 side panel (mortality of 2 ± 2%) and PermaNet3.0® roof (mortality of 16 ± 5.1%). Time-course bioassays conducted with deltamethrin established a high intensity of resistance, with LT50 of 309.09 (95% CI 253.07–393.71, Fiducial), and a resistance ratio of 93.09 compared with the fully susceptible Ngoussou laboratory colony. TaqMan genotyping revealed a high frequency of the 1014F allele (65.25%) in the An. coluzzii populations. Sequencing of a fragment of the voltage-gated sodium channel identified a single An. arabiensis female harbouring the 1014S kdr mutation. Conclusions This finding of high pyrethroid and DDT resistance in An. coluzzii from north-central Cameroon is a major obstacle to malaria control using pyrethroid bednets and indoor residual spraying with DDT.http://link.springer.com/article/10.1186/s13071-019-3523-7Anopheles coluzziiDeltamethrinPermethrinDDTMetabolic resistancekdr |
| spellingShingle | Amen N. Fadel Sulaiman S. Ibrahim Magellan Tchouakui Ebai Terence Murielle J. Wondji Micareme Tchoupo Samuel Wanji Charles S. Wondji A combination of metabolic resistance and high frequency of the 1014F kdr mutation is driving pyrethroid resistance in Anopheles coluzzii population from Guinea savanna of Cameroon Parasites & Vectors Anopheles coluzzii Deltamethrin Permethrin DDT Metabolic resistance kdr |
| title | A combination of metabolic resistance and high frequency of the 1014F kdr mutation is driving pyrethroid resistance in Anopheles coluzzii population from Guinea savanna of Cameroon |
| title_full | A combination of metabolic resistance and high frequency of the 1014F kdr mutation is driving pyrethroid resistance in Anopheles coluzzii population from Guinea savanna of Cameroon |
| title_fullStr | A combination of metabolic resistance and high frequency of the 1014F kdr mutation is driving pyrethroid resistance in Anopheles coluzzii population from Guinea savanna of Cameroon |
| title_full_unstemmed | A combination of metabolic resistance and high frequency of the 1014F kdr mutation is driving pyrethroid resistance in Anopheles coluzzii population from Guinea savanna of Cameroon |
| title_short | A combination of metabolic resistance and high frequency of the 1014F kdr mutation is driving pyrethroid resistance in Anopheles coluzzii population from Guinea savanna of Cameroon |
| title_sort | combination of metabolic resistance and high frequency of the 1014f kdr mutation is driving pyrethroid resistance in anopheles coluzzii population from guinea savanna of cameroon |
| topic | Anopheles coluzzii Deltamethrin Permethrin DDT Metabolic resistance kdr |
| url | http://link.springer.com/article/10.1186/s13071-019-3523-7 |
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