Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei: first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter]
David AB Dance,1 Vanaporn Wuthiekanun,2 Derek Sarovich,3 Erin P Price,3 Direk Limmathurotsakul,4 Bart J Currie,5 Trinh Thanh Trung61Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Vientiane, Lao PDR; 2Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Thailand; 3University...
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Dove Medical Press
2019-04-01
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| Series: | International Medical Case Reports Journal |
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| Online Access: | https://www.dovepress.com/httpswwwdovepresscomletter-pan-drug-resistant-and-biofilm-p-peer-reviewed-article-IMCRJ |
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| author | Dance DA Wuthiekanun V Sarovich D Price EP Limmathurotsakul D Currie BJ Trung TT |
| author_facet | Dance DA Wuthiekanun V Sarovich D Price EP Limmathurotsakul D Currie BJ Trung TT |
| author_sort | Dance DA |
| collection | DOAJ |
| description | David AB Dance,1 Vanaporn Wuthiekanun,2 Derek Sarovich,3 Erin P Price,3 Direk Limmathurotsakul,4 Bart J Currie,5 Trinh Thanh Trung61Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Vientiane, Lao PDR; 2Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Thailand; 3University of the Sunshine Coast, Sippy Downs, QLD, Australia; 4Microbiology, Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Thailand; 5Infectious Diseases, Menzies School of Health Research, Darwin, NT, Australia; 6Vietnam National University, Hanoi, VietnamWe are writing, on behalf of the International Melioidosis Society Committee, as a group of researchers and clinicians with longstanding experience of melioidosis and Burkholderia pseudomallei as we have some concerns about the above paper that was published in your journal recently.1 Although we believe that melioidosis is undoubtedly being under-diagnosed in Indonesia,2,3 we are not convinced that the isolate in this case is B.pseudomallei based on the information provided by the authors. Although it is difficult to be certain from the photographs in Figure 2, the colonies do not appear typical of the species to the microbiologists amongst us, who have seen several thousand isolates of B.pseudomallei over the past 30 years. Furthermore, the authors do not report whether the isolate was oxidase positive or negative. We believe that more comprehensive methods of confirming the identity, particularly genomic analysis, should have been undertaken before publishing the case.4 Unfortunately, the postamplification 16s analysis described in the paper might not have been able to distinguish between B.pseudomallei and other Burkholderia species (particularly B.thailandensis and several as-yet-uncharacterized Burkholderia spp.), and we would have recommended additional testing, for example, multilocus sequence typing and PCR for the TTS1 gene at least. View the original paper by Nuryastuti and colleagues. |
| first_indexed | 2024-12-19T13:41:19Z |
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| institution | Directory Open Access Journal |
| issn | 1179-142X |
| language | English |
| last_indexed | 2024-12-19T13:41:19Z |
| publishDate | 2019-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Medical Case Reports Journal |
| spelling | doaj.art-893632503b044ac3abf074ffa90b161e2022-12-21T20:19:00ZengDove Medical PressInternational Medical Case Reports Journal1179-142X2019-04-01Volume 1211711845212Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei: first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter]Dance DAWuthiekanun VSarovich DPrice EPLimmathurotsakul DCurrie BJTrung TT David AB Dance,1 Vanaporn Wuthiekanun,2 Derek Sarovich,3 Erin P Price,3 Direk Limmathurotsakul,4 Bart J Currie,5 Trinh Thanh Trung61Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Vientiane, Lao PDR; 2Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Thailand; 3University of the Sunshine Coast, Sippy Downs, QLD, Australia; 4Microbiology, Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Thailand; 5Infectious Diseases, Menzies School of Health Research, Darwin, NT, Australia; 6Vietnam National University, Hanoi, VietnamWe are writing, on behalf of the International Melioidosis Society Committee, as a group of researchers and clinicians with longstanding experience of melioidosis and Burkholderia pseudomallei as we have some concerns about the above paper that was published in your journal recently.1 Although we believe that melioidosis is undoubtedly being under-diagnosed in Indonesia,2,3 we are not convinced that the isolate in this case is B.pseudomallei based on the information provided by the authors. Although it is difficult to be certain from the photographs in Figure 2, the colonies do not appear typical of the species to the microbiologists amongst us, who have seen several thousand isolates of B.pseudomallei over the past 30 years. Furthermore, the authors do not report whether the isolate was oxidase positive or negative. We believe that more comprehensive methods of confirming the identity, particularly genomic analysis, should have been undertaken before publishing the case.4 Unfortunately, the postamplification 16s analysis described in the paper might not have been able to distinguish between B.pseudomallei and other Burkholderia species (particularly B.thailandensis and several as-yet-uncharacterized Burkholderia spp.), and we would have recommended additional testing, for example, multilocus sequence typing and PCR for the TTS1 gene at least. View the original paper by Nuryastuti and colleagues.https://www.dovepress.com/httpswwwdovepresscomletter-pan-drug-resistant-and-biofilm-p-peer-reviewed-article-IMCRJPseudomalleidrug resistancemelioidosisidentification |
| spellingShingle | Dance DA Wuthiekanun V Sarovich D Price EP Limmathurotsakul D Currie BJ Trung TT Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei: first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter] International Medical Case Reports Journal Pseudomallei drug resistance melioidosis identification |
| title | Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei: first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter] |
| title_full | Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei: first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter] |
| title_fullStr | Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei: first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter] |
| title_full_unstemmed | Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei: first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter] |
| title_short | Pan-drug-resistant and biofilm-producing strain of Burkholderia pseudomallei: first report of melioidosis from a diabetic patient in Yogyakarta, Indonesia [Letter] |
| title_sort | pan drug resistant and biofilm producing strain of burkholderia pseudomallei first report of melioidosis from a diabetic patient in yogyakarta indonesia letter |
| topic | Pseudomallei drug resistance melioidosis identification |
| url | https://www.dovepress.com/httpswwwdovepresscomletter-pan-drug-resistant-and-biofilm-p-peer-reviewed-article-IMCRJ |
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