Preservation of antigen-specific responses in cryopreserved CD4+ and CD8+ T cells expanded with IL-2 and IL-7

Preservation of antigen-specific responses in cryopreserved CD4+ and CD8+ T cells expanded with IL-2 and IL-7

Objectives: We sought to develop medium throughput standard operating procedures for screening cryopreserved human peripheral blood mononuclear cells (PBMCs) for CD4+ and CD8+ T cell responses to potential autoantigens. Methods: Dendritic cells were loaded with a peptide cocktail from ubiquitous vir...

Full description

Bibliographic Details
Main Authors: Benjamin DS. Clarkson, Renee K. Johnson, Corinna Bingel, Caroline Lothaller, Charles L. Howe
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:Journal of Translational Autoimmunity
Subjects:
T cell
Dendritic cells
Antigen stimulation
Monocytes
Polyclonal expansion
Online Access:http://www.sciencedirect.com/science/article/pii/S258990902200034X

Similar Items

Similar Items