A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest
This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), an...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Feira de Santana
2022-11-01
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| Series: | Sociobiology |
| Subjects: | |
| Online Access: | http://periodicos.uefs.br/index.php/sociobiology/article/view/8251 |
| _version_ | 1811207753987784704 |
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| author | Patricia Vit Bajaree Chuttong Norhasnida Zawawi Maria Diaz Jane van der Meulen Hajar F. Ahmad Francisco A. Tomas-Barberan Gina Meccia Khanchai Danmek Jorge Enrique Moreno David W. Roubik Ortrud Monika Barth Dirk W. Lachenmeier Michael S. Engel |
| author_facet | Patricia Vit Bajaree Chuttong Norhasnida Zawawi Maria Diaz Jane van der Meulen Hajar F. Ahmad Francisco A. Tomas-Barberan Gina Meccia Khanchai Danmek Jorge Enrique Moreno David W. Roubik Ortrud Monika Barth Dirk W. Lachenmeier Michael S. Engel |
| author_sort | Patricia Vit |
| collection | DOAJ |
| description |
This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations.
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| first_indexed | 2024-04-12T04:09:51Z |
| format | Article |
| id | doaj.art-8938ed24d8c9418b9cf2b9eff59786bb |
| institution | Directory Open Access Journal |
| issn | 0361-6525 2447-8067 |
| language | English |
| last_indexed | 2024-04-12T04:09:51Z |
| publishDate | 2022-11-01 |
| publisher | Universidade Estadual de Feira de Santana |
| record_format | Article |
| series | Sociobiology |
| spelling | doaj.art-8938ed24d8c9418b9cf2b9eff59786bb2022-12-22T03:48:32ZengUniversidade Estadual de Feira de SantanaSociobiology0361-65252447-80672022-11-0169410.13102/sociobiology.v64i4.8251A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvestPatricia Vit0Bajaree Chuttong1Norhasnida Zawawi2Maria Diaz3Jane van der Meulen4Hajar F. Ahmad5Francisco A. Tomas-Barberan6Gina Meccia7Khanchai Danmek8Jorge Enrique Moreno9David W. Roubik10Ortrud Monika Barth11Dirk W. Lachenmeier12Michael S. Engel13Universidad de Los AndesMeliponini and Apini Research Laboratory, Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.Faculty of Food Science and Technology, Universiti Putra Malaysia, Selangor, 43400, MalaysiaQuality Services International GmbH, 28199 Bremen, Germany.Quality Services International GmbH, 28199 Bremen, GermanyUniversiti Malaysia PahangDepartment of Food Science and Technology, CEBAS-CSIC, Campus Universitario de Espinardo, Edf 25, 30100 Murcia, SpainUniversity of the AndesSchool of Agriculture and Natural Resources, University of Phayao, Phayao 56000 ThailandSmithsonian Tropical Research Institute, Balboa, Ancon, Republic of PanamaSmithsonian Tropical Research Institute, Balboa, Ancon, Republic of PanamaInstituto Oswaldo Cruz, Fundação Oswaldo Cruz, 21040-900 Rio de Janeiro, BrazilChemical and Veterinary Investigation Agency CVUA, Karlsruhe, Germany11Division of Entomology, Natural History Museum, and Department of Ecology & Evolutionary Biology, 1501 Crestline Drive-Suite 140, University of Kansas, Lawrence, Kansas 66045, USA. 12Division of Invertebrate Zoology, American Museum of Natural History, Central Park West at 79th Street, New York, New York 10024, USA. This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations. http://periodicos.uefs.br/index.php/sociobiology/article/view/8251antioxidant activitybiosurfactant activity1H-NMRflavonoidsMeliponinimelyssopalinology |
| spellingShingle | Patricia Vit Bajaree Chuttong Norhasnida Zawawi Maria Diaz Jane van der Meulen Hajar F. Ahmad Francisco A. Tomas-Barberan Gina Meccia Khanchai Danmek Jorge Enrique Moreno David W. Roubik Ortrud Monika Barth Dirk W. Lachenmeier Michael S. Engel A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest Sociobiology antioxidant activity biosurfactant activity 1H-NMR flavonoids Meliponini melyssopalinology |
| title | A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title_full | A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title_fullStr | A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title_full_unstemmed | A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title_short | A Novel Integrative Methodology for Research on Pot-honey Variations During Post-harvest |
| title_sort | novel integrative methodology for research on pot honey variations during post harvest |
| topic | antioxidant activity biosurfactant activity 1H-NMR flavonoids Meliponini melyssopalinology |
| url | http://periodicos.uefs.br/index.php/sociobiology/article/view/8251 |
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