Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease Virus
A total of 200 one-day-old male broilers were assigned to five groups, and each group consisted of four replicates with 10 birds per replicate. Chicks were fed the basal diet with 0 (non-infected control), 0 (infected control), 10, 20, and 40 mg/kg soybean isoflavones (SI) for 42 days. At 21 days of...
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MDPI AG
2019-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/5/247 |
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| author | Mahmoud Mostafa Azzam Shou-qun JIANG Jia-li CHEN Xia-jing LIN Zhong-yong GOU Qiu-li FAN Yi-bing WANG Long LI Zong-yong JIANG |
| author_facet | Mahmoud Mostafa Azzam Shou-qun JIANG Jia-li CHEN Xia-jing LIN Zhong-yong GOU Qiu-li FAN Yi-bing WANG Long LI Zong-yong JIANG |
| author_sort | Mahmoud Mostafa Azzam |
| collection | DOAJ |
| description | A total of 200 one-day-old male broilers were assigned to five groups, and each group consisted of four replicates with 10 birds per replicate. Chicks were fed the basal diet with 0 (non-infected control), 0 (infected control), 10, 20, and 40 mg/kg soybean isoflavones (SI) for 42 days. At 21 days of age, chickens were inoculated with an infectious bursal dose (causing 50% morbidity) of the infectious bursal disease virus (IBDV) BC 6/85 strain by the eye-drop and nasal route (except for the non-infected group). Average daily gain (ADG) and average daily feed intake (ADFI) decreased (<i>p</i> < 0.05) in broilers infected with infectious bursal disease virus (IBDV) from 22 to 42 days. However, infected broilers fed 10 and 20 mg SI/kg had the maximum (<i>p</i> <0.05) ADG and ADFI from 1 to 42 days. Body weight (BW) increased (<i>p</i> < 0.05) in infected broilers fed the 10 and 20 mg SI /kg diet. The bursa weight at 7 days post-infection (dpi) was increased (<i>p</i> < 0.05) by the supplemental 10 mg SI/kg diet. Infected broilers showed the highest (<i>p</i> < 0.05) bursa lesions, with an average score of 4.0 ± 0.0, while the severity of bursa lesions was decreased (<i>p</i> < 0.05) at 3 dpi and 7 dpi by the supplemental 20 mg SI/kg diet. Supplemental SI at 20 mg/kg decreased (<i>p</i> < 0.05) the viral protein 5 (VP5) mRNA expression at 3 dpi and 7 dpi. The level of interferon gamma (IFNγ) was elevated (<i>p</i> < 0.05) in the infected group at 3 dpi and 7 dpi as compared with the control group, while its level was decreased <i>(p</i> < 0.05) by supplemental 10 mg/kg SI at 3 dpi. The level of nuclear factor κB in the bursal tissue showed the lowest value (<i>p</i> < 0.05) with supplemental 10 and 20 mg SI/kg diet at 7 dpi. Supplemental 10, 20, 40 mg/kg SI improved (<i>p</i> < 0.05) the serum total antioxidant activity (T-AOC) in infected broilers at 3 dpi. In addition, the serum level of malondialdehyde (MDA) decreased (<i>p</i> < 0.05) in the group fed 20 mg/kg SI at 7 dpi. In conclusion, supplemental 10~20 mg/kg SI may have a positive effect on broiler chickens infected with IBDV, probably because SI decrease the severity of bursa lesions and viral protein 5 mRNA expression, and have strong antioxidant activity. |
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| spelling | doaj.art-893a2cc6ad1c484b80a9b0ac1da9e93a2022-12-22T03:16:44ZengMDPI AGAnimals2076-26152019-05-019524710.3390/ani9050247ani9050247Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease VirusMahmoud Mostafa Azzam0Shou-qun JIANG1Jia-li CHEN2Xia-jing LIN3Zhong-yong GOU4Qiu-li FAN5Yi-bing WANG6Long LI7Zong-yong JIANG8Guangdong Key Laboratory of Animal Breeding and Nutrition/Guangdong Public Laboratory of Animal Breeding and Nutrition/The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture/State Key Laboratory of Livestock and Poultry Breeding/Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition/Guangdong Public Laboratory of Animal Breeding and Nutrition/The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture/State Key Laboratory of Livestock and Poultry Breeding/Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition/Guangdong Public Laboratory of Animal Breeding and Nutrition/The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture/State Key Laboratory of Livestock and Poultry Breeding/Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition/Guangdong Public Laboratory of Animal Breeding and Nutrition/The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture/State Key Laboratory of Livestock and Poultry Breeding/Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition/Guangdong Public Laboratory of Animal Breeding and Nutrition/The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture/State Key Laboratory of Livestock and Poultry Breeding/Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition/Guangdong Public Laboratory of Animal Breeding and Nutrition/The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture/State Key Laboratory of Livestock and Poultry Breeding/Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition/Guangdong Public Laboratory of Animal Breeding and Nutrition/The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture/State Key Laboratory of Livestock and Poultry Breeding/Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition/Guangdong Public Laboratory of Animal Breeding and Nutrition/The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture/State Key Laboratory of Livestock and Poultry Breeding/Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition/Guangdong Public Laboratory of Animal Breeding and Nutrition/The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture/State Key Laboratory of Livestock and Poultry Breeding/Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaA total of 200 one-day-old male broilers were assigned to five groups, and each group consisted of four replicates with 10 birds per replicate. Chicks were fed the basal diet with 0 (non-infected control), 0 (infected control), 10, 20, and 40 mg/kg soybean isoflavones (SI) for 42 days. At 21 days of age, chickens were inoculated with an infectious bursal dose (causing 50% morbidity) of the infectious bursal disease virus (IBDV) BC 6/85 strain by the eye-drop and nasal route (except for the non-infected group). Average daily gain (ADG) and average daily feed intake (ADFI) decreased (<i>p</i> < 0.05) in broilers infected with infectious bursal disease virus (IBDV) from 22 to 42 days. However, infected broilers fed 10 and 20 mg SI/kg had the maximum (<i>p</i> <0.05) ADG and ADFI from 1 to 42 days. Body weight (BW) increased (<i>p</i> < 0.05) in infected broilers fed the 10 and 20 mg SI /kg diet. The bursa weight at 7 days post-infection (dpi) was increased (<i>p</i> < 0.05) by the supplemental 10 mg SI/kg diet. Infected broilers showed the highest (<i>p</i> < 0.05) bursa lesions, with an average score of 4.0 ± 0.0, while the severity of bursa lesions was decreased (<i>p</i> < 0.05) at 3 dpi and 7 dpi by the supplemental 20 mg SI/kg diet. Supplemental SI at 20 mg/kg decreased (<i>p</i> < 0.05) the viral protein 5 (VP5) mRNA expression at 3 dpi and 7 dpi. The level of interferon gamma (IFNγ) was elevated (<i>p</i> < 0.05) in the infected group at 3 dpi and 7 dpi as compared with the control group, while its level was decreased <i>(p</i> < 0.05) by supplemental 10 mg/kg SI at 3 dpi. The level of nuclear factor κB in the bursal tissue showed the lowest value (<i>p</i> < 0.05) with supplemental 10 and 20 mg SI/kg diet at 7 dpi. Supplemental 10, 20, 40 mg/kg SI improved (<i>p</i> < 0.05) the serum total antioxidant activity (T-AOC) in infected broilers at 3 dpi. In addition, the serum level of malondialdehyde (MDA) decreased (<i>p</i> < 0.05) in the group fed 20 mg/kg SI at 7 dpi. In conclusion, supplemental 10~20 mg/kg SI may have a positive effect on broiler chickens infected with IBDV, probably because SI decrease the severity of bursa lesions and viral protein 5 mRNA expression, and have strong antioxidant activity.https://www.mdpi.com/2076-2615/9/5/247broilersIBDVsoybean isoflavonesimmune functionviral protein 5 mRNA expression |
| spellingShingle | Mahmoud Mostafa Azzam Shou-qun JIANG Jia-li CHEN Xia-jing LIN Zhong-yong GOU Qiu-li FAN Yi-bing WANG Long LI Zong-yong JIANG Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease Virus Animals broilers IBDV soybean isoflavones immune function viral protein 5 mRNA expression |
| title | Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease Virus |
| title_full | Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease Virus |
| title_fullStr | Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease Virus |
| title_full_unstemmed | Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease Virus |
| title_short | Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease Virus |
| title_sort | effect of soybean isoflavones on growth performance immune function and viral protein 5 mrna expression in broiler chickens challenged with infectious bursal disease virus |
| topic | broilers IBDV soybean isoflavones immune function viral protein 5 mRNA expression |
| url | https://www.mdpi.com/2076-2615/9/5/247 |
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