Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus peruvianus Mill. and Using Tissue Culture Technique
Background: The extracted juice and alkaloeids of the medicinal and ornamental cactus Cereus peruvianus are contain a lot of effective compounds which widely use in medicine and agricultural industries. Propagation of this plant using conventional method (seed, cutting, offset) is very slow and its...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2010-05-01
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| Series: | Journal of Medicinal Plants |
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| Online Access: | http://jmp.ir/article-1-282-en.html |
| _version_ | 1819122339709714432 |
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| author | N Karimi R Naderi M Ebrahimi MR Mofid |
| author_facet | N Karimi R Naderi M Ebrahimi MR Mofid |
| author_sort | N Karimi |
| collection | DOAJ |
| description | Background: The extracted juice and alkaloeids of the medicinal and ornamental cactus Cereus peruvianus are contain a lot of effective compounds which widely use in medicine and agricultural industries. Propagation of this plant using conventional method (seed, cutting, offset) is very slow and its mass production is possible by tissue culture technique. Objective: The aim of this study was the micropropagation possibility and recognition of some of its different aspects, and determination of suitable medium and hormonal composition at in-vitro conditions of cereus. Methods: The experimental design was compeletly randomized with 3 replication and each replicate was contained 5 sample. After surface disinfection the explants were cultured on MS basal medium containing %3 sucrose,8 g/l phytoagar and different treatment of plant growth regulators (IBA, NAA, Kinetin, BAP, 2,4-D, GA3, TDZ) and pH=5/8. After 45 days, some parameters such as effectivness of culture media in percent of regeneration from areole, production of friable callus and rooting in all treatments were recorded. Results: In this research The best growth of friable callus was obtained in MS medium supplemented with 0.07mg/l TDZ and 0.05 mg/l NAA and 4mg/l 2,4-D with 6mg/l kinetin for regeneration of areole and 4mg/l 2,4-D and 4mg/l kinetin for rooting was the most favorable medium.High regeneration potential was observed after 2.5-3 months of culture. Eight different media was selected for plantlet regeneration.There is a significant difference between regeneration of areole in cereus peruvianus var monstrosus and cereus peruvianus var tortuosus. Conclusion: As for to the importance of the extracted juice and alkaloeids of the cactus Cereus peruvianus Propagation of this plant using by conventional method is very slow and propagation it of areol in addition to economical is very fast. |
| first_indexed | 2024-12-22T06:50:53Z |
| format | Article |
| id | doaj.art-893ae5558f85482f8b2427d2723ada9f |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-22T06:50:53Z |
| publishDate | 2010-05-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-893ae5558f85482f8b2427d2723ada9f2022-12-21T18:35:10ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582010-05-019343845Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus peruvianus Mill. and Using Tissue Culture TechniqueN Karimi0R Naderi1M Ebrahimi2MR Mofid3 Faculty of Horticultural Sciences, Islamic Azad University (Karaj Branch) Faculty of Horticultural Sciences, University of Tehran Agriculture Biotechnology Institute of Iran-Central Region Department of Biochemistry, School of Pharmacy, Isfahan University of medical sciences Background: The extracted juice and alkaloeids of the medicinal and ornamental cactus Cereus peruvianus are contain a lot of effective compounds which widely use in medicine and agricultural industries. Propagation of this plant using conventional method (seed, cutting, offset) is very slow and its mass production is possible by tissue culture technique. Objective: The aim of this study was the micropropagation possibility and recognition of some of its different aspects, and determination of suitable medium and hormonal composition at in-vitro conditions of cereus. Methods: The experimental design was compeletly randomized with 3 replication and each replicate was contained 5 sample. After surface disinfection the explants were cultured on MS basal medium containing %3 sucrose,8 g/l phytoagar and different treatment of plant growth regulators (IBA, NAA, Kinetin, BAP, 2,4-D, GA3, TDZ) and pH=5/8. After 45 days, some parameters such as effectivness of culture media in percent of regeneration from areole, production of friable callus and rooting in all treatments were recorded. Results: In this research The best growth of friable callus was obtained in MS medium supplemented with 0.07mg/l TDZ and 0.05 mg/l NAA and 4mg/l 2,4-D with 6mg/l kinetin for regeneration of areole and 4mg/l 2,4-D and 4mg/l kinetin for rooting was the most favorable medium.High regeneration potential was observed after 2.5-3 months of culture. Eight different media was selected for plantlet regeneration.There is a significant difference between regeneration of areole in cereus peruvianus var monstrosus and cereus peruvianus var tortuosus. Conclusion: As for to the importance of the extracted juice and alkaloeids of the cactus Cereus peruvianus Propagation of this plant using by conventional method is very slow and propagation it of areol in addition to economical is very fast.http://jmp.ir/article-1-282-en.htmlcereus peruvianusvarite monstrosus and tortuosuscallusmicropropagation |
| spellingShingle | N Karimi R Naderi M Ebrahimi MR Mofid Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus peruvianus Mill. and Using Tissue Culture Technique Journal of Medicinal Plants cereus peruvianus varite monstrosus and tortuosus callus micropropagation |
| title | Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus peruvianus Mill. and Using Tissue Culture Technique |
| title_full | Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus peruvianus Mill. and Using Tissue Culture Technique |
| title_fullStr | Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus peruvianus Mill. and Using Tissue Culture Technique |
| title_full_unstemmed | Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus peruvianus Mill. and Using Tissue Culture Technique |
| title_short | Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus peruvianus Mill. and Using Tissue Culture Technique |
| title_sort | investigation of a medicinal and ornamental plant propagation the cereus peruvianus mill and using tissue culture technique |
| topic | cereus peruvianus varite monstrosus and tortuosus callus micropropagation |
| url | http://jmp.ir/article-1-282-en.html |
| work_keys_str_mv | AT nkarimi investigationofamedicinalandornamentalplantpropagationthecereusperuvianusmillandusingtissueculturetechnique AT rnaderi investigationofamedicinalandornamentalplantpropagationthecereusperuvianusmillandusingtissueculturetechnique AT mebrahimi investigationofamedicinalandornamentalplantpropagationthecereusperuvianusmillandusingtissueculturetechnique AT mrmofid investigationofamedicinalandornamentalplantpropagationthecereusperuvianusmillandusingtissueculturetechnique |