Physicochemical and functional properties of pulp and pectin from agro-waste of three Cucurbitaceae species
Cucurbita pepo L., Citrullus lanatus T. and Cucumis melo L. belong to the family Cucurbitaceae and are cultivated for their seeds. The fruit pulp and peel are discarded as agro-waste after seed removal. This study evaluated the physicochemical and functional properties of the pulp and pectin extract...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Food Chemistry Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X23003519 |
| _version_ | 1797382789548474368 |
|---|---|
| author | Fidelis M. Kpodo Jonathan Jato Clementina Naa Adjeley Adjei Azi Walter Jacob K. Agbenorhevi Joyce Duah Peter Nuro-Ameyaw |
| author_facet | Fidelis M. Kpodo Jonathan Jato Clementina Naa Adjeley Adjei Azi Walter Jacob K. Agbenorhevi Joyce Duah Peter Nuro-Ameyaw |
| author_sort | Fidelis M. Kpodo |
| collection | DOAJ |
| description | Cucurbita pepo L., Citrullus lanatus T. and Cucumis melo L. belong to the family Cucurbitaceae and are cultivated for their seeds. The fruit pulp and peel are discarded as agro-waste after seed removal. This study evaluated the physicochemical and functional properties of the pulp and pectin extracts from the Cucurbitaceae species. Proximate, antioxidant, total polyphenol and flavonoid contents were determined. The purity, structural and sugar constituents of the extracted pectin were analysed using spectrophotometric techniques (NMR, FTIR and LCMS). Results showed that the pulp wastes had high carbohydrate content (28.4 to 69.7%) and demonstrated high antioxidant activities (40 to 50%, 63 to 85% and 0.2 to 0.4 mg mL−1 for DPPH, ABTS and FRAP respectively). Cucurbita pepo L. had the highest pectin yield (13.9%), and the purity of the pectin extracts ranged from 60.4 to 75.2% for total carbohydrate and 3.3 to 4.4 % for protein. The pectin extracts showed structural similarities. LCMS fingerprints of the pectin extracts showed that the monosaccharides comprised of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose, arabinose and fucose. The polymers demonstrated high water absorption capacities (309 g/100 g to 604 g/100 g) and can be applied as thickeners in food systems. |
| first_indexed | 2024-03-08T21:10:32Z |
| format | Article |
| id | doaj.art-893c93200292421f9d129a0f5de5c011 |
| institution | Directory Open Access Journal |
| issn | 2772-753X |
| language | English |
| last_indexed | 2024-03-08T21:10:32Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj.art-893c93200292421f9d129a0f5de5c0112023-12-22T05:35:20ZengElsevierFood Chemistry Advances2772-753X2023-12-013100530Physicochemical and functional properties of pulp and pectin from agro-waste of three Cucurbitaceae speciesFidelis M. Kpodo0Jonathan Jato1Clementina Naa Adjeley Adjei2Azi Walter3Jacob K. Agbenorhevi4Joyce Duah5Peter Nuro-Ameyaw6Department of Nutrition and Dietetics, University of Health and Allied Sciences, Ho, Ghana; Corresponding author.Department of Pharmacognosy & Herbal Medicine, University of Health and Allied Sciences, Ho, GhanaDepartment of Nutrition and Dietetics, University of Health and Allied Sciences, Ho, GhanaDepartment of Nutrition and Dietetics, University of Health and Allied Sciences, Ho, GhanaDepartment of Food Science and Technology, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Food Science and Technology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana; Department of Nutrition and Food Science, University of Ghana, Accra, GhanaDepartment of Nutrition and Dietetics, University of Health and Allied Sciences, Ho, GhanaCucurbita pepo L., Citrullus lanatus T. and Cucumis melo L. belong to the family Cucurbitaceae and are cultivated for their seeds. The fruit pulp and peel are discarded as agro-waste after seed removal. This study evaluated the physicochemical and functional properties of the pulp and pectin extracts from the Cucurbitaceae species. Proximate, antioxidant, total polyphenol and flavonoid contents were determined. The purity, structural and sugar constituents of the extracted pectin were analysed using spectrophotometric techniques (NMR, FTIR and LCMS). Results showed that the pulp wastes had high carbohydrate content (28.4 to 69.7%) and demonstrated high antioxidant activities (40 to 50%, 63 to 85% and 0.2 to 0.4 mg mL−1 for DPPH, ABTS and FRAP respectively). Cucurbita pepo L. had the highest pectin yield (13.9%), and the purity of the pectin extracts ranged from 60.4 to 75.2% for total carbohydrate and 3.3 to 4.4 % for protein. The pectin extracts showed structural similarities. LCMS fingerprints of the pectin extracts showed that the monosaccharides comprised of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose, arabinose and fucose. The polymers demonstrated high water absorption capacities (309 g/100 g to 604 g/100 g) and can be applied as thickeners in food systems.http://www.sciencedirect.com/science/article/pii/S2772753X23003519Cucurbitaceae agro-wasteCucurbitaceae pectinAntioxidant characterization |
| spellingShingle | Fidelis M. Kpodo Jonathan Jato Clementina Naa Adjeley Adjei Azi Walter Jacob K. Agbenorhevi Joyce Duah Peter Nuro-Ameyaw Physicochemical and functional properties of pulp and pectin from agro-waste of three Cucurbitaceae species Food Chemistry Advances Cucurbitaceae agro-waste Cucurbitaceae pectin Antioxidant characterization |
| title | Physicochemical and functional properties of pulp and pectin from agro-waste of three Cucurbitaceae species |
| title_full | Physicochemical and functional properties of pulp and pectin from agro-waste of three Cucurbitaceae species |
| title_fullStr | Physicochemical and functional properties of pulp and pectin from agro-waste of three Cucurbitaceae species |
| title_full_unstemmed | Physicochemical and functional properties of pulp and pectin from agro-waste of three Cucurbitaceae species |
| title_short | Physicochemical and functional properties of pulp and pectin from agro-waste of three Cucurbitaceae species |
| title_sort | physicochemical and functional properties of pulp and pectin from agro waste of three cucurbitaceae species |
| topic | Cucurbitaceae agro-waste Cucurbitaceae pectin Antioxidant characterization |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X23003519 |
| work_keys_str_mv | AT fidelismkpodo physicochemicalandfunctionalpropertiesofpulpandpectinfromagrowasteofthreecucurbitaceaespecies AT jonathanjato physicochemicalandfunctionalpropertiesofpulpandpectinfromagrowasteofthreecucurbitaceaespecies AT clementinanaaadjeleyadjei physicochemicalandfunctionalpropertiesofpulpandpectinfromagrowasteofthreecucurbitaceaespecies AT aziwalter physicochemicalandfunctionalpropertiesofpulpandpectinfromagrowasteofthreecucurbitaceaespecies AT jacobkagbenorhevi physicochemicalandfunctionalpropertiesofpulpandpectinfromagrowasteofthreecucurbitaceaespecies AT joyceduah physicochemicalandfunctionalpropertiesofpulpandpectinfromagrowasteofthreecucurbitaceaespecies AT peternuroameyaw physicochemicalandfunctionalpropertiesofpulpandpectinfromagrowasteofthreecucurbitaceaespecies |