In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract
Abstract Standard cell culture practices require the addition of animal-derived serum to culture media to achieve adequate cell growth. Typically, 5–10% by volume of fetal bovine serum (FBS) is used, which accounts for a vast majority of the media cost while also imposing environmental and ethical c...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12896-023-00774-w |
| _version_ | 1811165857194180608 |
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| author | Galileo Defendi-Cho Timothy M. Gould |
| author_facet | Galileo Defendi-Cho Timothy M. Gould |
| author_sort | Galileo Defendi-Cho |
| collection | DOAJ |
| description | Abstract Standard cell culture practices require the addition of animal-derived serum to culture media to achieve adequate cell growth. Typically, 5–10% by volume of fetal bovine serum (FBS) is used, which accounts for a vast majority of the media cost while also imposing environmental and ethical concerns associated with the use of animal serum. Here we tested the efficacy of culturing cells by replacing serum in the media with algae extract and select additives. Using LC–MS, we compared molecular signatures of FBS to Chlorella algae extracts and identified NAD(H)/NADP(H) as common and relatively abundant features in their characteristic profiles. Bovine fibroblasts, cultured in serum-free media supplemented with C. vulgaris extract and just two growth factors plus insulin, showed significant growth with enhanced viability compared to control cells cultured without serum, albeit still lower than that of controls cultured with 10% FBS. Moreover, C. vulgaris extract enhanced cell viability beyond that of cells cultured with the two growth factors and insulin alone. These results suggest that key components in serum which are essential for cell growth may also be present in C. vulgaris extract, demonstrating that it may be used at least as a partial alternative to serum for cell culture applications. |
| first_indexed | 2024-04-10T15:43:19Z |
| format | Article |
| id | doaj.art-893d941ca4234b448e581de3650f9635 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-04-10T15:43:19Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-893d941ca4234b448e581de3650f96352023-02-12T12:15:37ZengBMCBMC Biotechnology1472-67502023-02-0123111110.1186/s12896-023-00774-wIn vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extractGalileo Defendi-Cho0Timothy M. Gould1Department of Chemistry and Biochemistry, Colorado CollegeDepartment of Chemistry and Biochemistry, Colorado CollegeAbstract Standard cell culture practices require the addition of animal-derived serum to culture media to achieve adequate cell growth. Typically, 5–10% by volume of fetal bovine serum (FBS) is used, which accounts for a vast majority of the media cost while also imposing environmental and ethical concerns associated with the use of animal serum. Here we tested the efficacy of culturing cells by replacing serum in the media with algae extract and select additives. Using LC–MS, we compared molecular signatures of FBS to Chlorella algae extracts and identified NAD(H)/NADP(H) as common and relatively abundant features in their characteristic profiles. Bovine fibroblasts, cultured in serum-free media supplemented with C. vulgaris extract and just two growth factors plus insulin, showed significant growth with enhanced viability compared to control cells cultured without serum, albeit still lower than that of controls cultured with 10% FBS. Moreover, C. vulgaris extract enhanced cell viability beyond that of cells cultured with the two growth factors and insulin alone. These results suggest that key components in serum which are essential for cell growth may also be present in C. vulgaris extract, demonstrating that it may be used at least as a partial alternative to serum for cell culture applications.https://doi.org/10.1186/s12896-023-00774-wSerum replacementSerum-free mediaAlgae extractBovine fibroblastCell-cultured meat |
| spellingShingle | Galileo Defendi-Cho Timothy M. Gould In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract BMC Biotechnology Serum replacement Serum-free media Algae extract Bovine fibroblast Cell-cultured meat |
| title | In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract |
| title_full | In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract |
| title_fullStr | In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract |
| title_full_unstemmed | In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract |
| title_short | In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract |
| title_sort | in vitro culture of bovine fibroblasts using select serum free media supplemented with chlorella vulgaris extract |
| topic | Serum replacement Serum-free media Algae extract Bovine fibroblast Cell-cultured meat |
| url | https://doi.org/10.1186/s12896-023-00774-w |
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