AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions.
Morphometry characterization is an important procedure in describing neuronal cultures and identifying phenotypic differences. This task usually requires labor-intensive measurements and the classification of numerous neurites from large numbers of neurons in culture. To automate these measurements,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0234529 |
| _version_ | 1819125949208199168 |
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| author | Benoit Boulan Anne Beghin Charlotte Ravanello Jean-Christophe Deloulme Sylvie Gory-Fauré Annie Andrieux Jacques Brocard Eric Denarier |
| author_facet | Benoit Boulan Anne Beghin Charlotte Ravanello Jean-Christophe Deloulme Sylvie Gory-Fauré Annie Andrieux Jacques Brocard Eric Denarier |
| author_sort | Benoit Boulan |
| collection | DOAJ |
| description | Morphometry characterization is an important procedure in describing neuronal cultures and identifying phenotypic differences. This task usually requires labor-intensive measurements and the classification of numerous neurites from large numbers of neurons in culture. To automate these measurements, we wrote AutoNeuriteJ, an imageJ/Fiji plugin that measures and classifies neurites from a very large number of neurons. We showed that AutoNeuriteJ is able to detect variations of neuritic growth induced by several compounds known to affect the neuronal growth. In these experiments measurement of more than 5000 mouse neurons per conditions was obtained within a few hours. Moreover, by analyzing mouse neurons deficient for the microtubule associated protein 6 (MAP6) and wild type neurons we illustrate that AutoNeuriteJ is capable to detect subtle phenotypic difference in axonal length. Overall the use of AutoNeuriteJ will provide rapid, unbiased and accurate measurement of neuron morphologies. |
| first_indexed | 2024-12-22T07:48:16Z |
| format | Article |
| id | doaj.art-893e99dfad844856ba43387126c2d177 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T07:48:16Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-893e99dfad844856ba43387126c2d1772022-12-21T18:33:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01157e023452910.1371/journal.pone.0234529AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions.Benoit BoulanAnne BeghinCharlotte RavanelloJean-Christophe DeloulmeSylvie Gory-FauréAnnie AndrieuxJacques BrocardEric DenarierMorphometry characterization is an important procedure in describing neuronal cultures and identifying phenotypic differences. This task usually requires labor-intensive measurements and the classification of numerous neurites from large numbers of neurons in culture. To automate these measurements, we wrote AutoNeuriteJ, an imageJ/Fiji plugin that measures and classifies neurites from a very large number of neurons. We showed that AutoNeuriteJ is able to detect variations of neuritic growth induced by several compounds known to affect the neuronal growth. In these experiments measurement of more than 5000 mouse neurons per conditions was obtained within a few hours. Moreover, by analyzing mouse neurons deficient for the microtubule associated protein 6 (MAP6) and wild type neurons we illustrate that AutoNeuriteJ is capable to detect subtle phenotypic difference in axonal length. Overall the use of AutoNeuriteJ will provide rapid, unbiased and accurate measurement of neuron morphologies.https://doi.org/10.1371/journal.pone.0234529 |
| spellingShingle | Benoit Boulan Anne Beghin Charlotte Ravanello Jean-Christophe Deloulme Sylvie Gory-Fauré Annie Andrieux Jacques Brocard Eric Denarier AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions. PLoS ONE |
| title | AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions. |
| title_full | AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions. |
| title_fullStr | AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions. |
| title_full_unstemmed | AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions. |
| title_short | AutoNeuriteJ: An ImageJ plugin for measurement and classification of neuritic extensions. |
| title_sort | autoneuritej an imagej plugin for measurement and classification of neuritic extensions |
| url | https://doi.org/10.1371/journal.pone.0234529 |
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