Effects of Aerobic Exercise on the Autophagy-Related Protein Expressions in Vascular Endothelial Cells
PURPOSE Autophagy is a highly conserved process that maintains cellular energy and redox homeostasis by degrading damaged cytosolic constituents in response to external stress. The purpose of this study was to determine the effect of exercise-induced laminar shear stress (LSS) on the autophagy-relat...
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| Format: | Article |
| Language: | Korean |
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The Korean Society of Exercise Physiology
2018-05-01
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| Series: | 운동과학 |
| Subjects: | |
| Online Access: | http://www.ksep-es.org/upload/pdf/es-27-2-140.pdf |
| _version_ | 1818315052128665600 |
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| author | Ji-Seok Kim Kijeong Kim Hojun Lee |
| author_facet | Ji-Seok Kim Kijeong Kim Hojun Lee |
| author_sort | Ji-Seok Kim |
| collection | DOAJ |
| description | PURPOSE Autophagy is a highly conserved process that maintains cellular energy and redox homeostasis by degrading damaged cytosolic constituents in response to external stress. The purpose of this study was to determine the effect of exercise-induced laminar shear stress (LSS) on the autophagy-related protein expressions in vascular endothelial cells. METHODS The orbital-shaker LSS was applied to the cultured human aortic endothelial cells (HAECs) or human umbilical vein endothelial cells (HUVECs) in the intensity of 20 dyne/cm2 for 24 hours. LSS was estimated as: τmax=α√ (p×η (2πf)3). Ten C57BL/6 male mice were subjected to the voluntary wheel running for 12 weeks, while the other ten mice were used as sedentary control. The protein expressions of autophagy-related genes were determined by western-blotting. RESULTS In HAECs, Atg3 expression was significantly increased by LSS, while Atg7 and LC3II only showed increased patterns. In HUVECs, expressions of Atg3 and LC3II were significantly increased, while p62 was significantly decreased. In exercised mice, Atg3, Atg7 and LC3II expressions were significantly increased. CONCLUSIONS The current study has shown that the exercise-induced increased LSS enhances the level of autophagy-related protein expressions in vascular endothelial cells. |
| first_indexed | 2024-12-13T08:59:24Z |
| format | Article |
| id | doaj.art-89440031840a47a6ab2c11c8b30d89e4 |
| institution | Directory Open Access Journal |
| issn | 1226-1726 2384-0544 |
| language | Korean |
| last_indexed | 2024-12-13T08:59:24Z |
| publishDate | 2018-05-01 |
| publisher | The Korean Society of Exercise Physiology |
| record_format | Article |
| series | 운동과학 |
| spelling | doaj.art-89440031840a47a6ab2c11c8b30d89e42022-12-21T23:53:12ZkorThe Korean Society of Exercise Physiology운동과학1226-17262384-05442018-05-0127214014510.15857/ksep.2018.27.2.140744Effects of Aerobic Exercise on the Autophagy-Related Protein Expressions in Vascular Endothelial CellsJi-Seok Kim0Kijeong Kim1Hojun Lee2Department of Physical Education, Gyeongsang National University, Jinju, KoreaSchool of Exercise & Sport Science, University of Ulsan, Ulsan, KoreaDivision of Sports and Health Science, Kyungsung University, Busan, KoreaPURPOSE Autophagy is a highly conserved process that maintains cellular energy and redox homeostasis by degrading damaged cytosolic constituents in response to external stress. The purpose of this study was to determine the effect of exercise-induced laminar shear stress (LSS) on the autophagy-related protein expressions in vascular endothelial cells. METHODS The orbital-shaker LSS was applied to the cultured human aortic endothelial cells (HAECs) or human umbilical vein endothelial cells (HUVECs) in the intensity of 20 dyne/cm2 for 24 hours. LSS was estimated as: τmax=α√ (p×η (2πf)3). Ten C57BL/6 male mice were subjected to the voluntary wheel running for 12 weeks, while the other ten mice were used as sedentary control. The protein expressions of autophagy-related genes were determined by western-blotting. RESULTS In HAECs, Atg3 expression was significantly increased by LSS, while Atg7 and LC3II only showed increased patterns. In HUVECs, expressions of Atg3 and LC3II were significantly increased, while p62 was significantly decreased. In exercised mice, Atg3, Atg7 and LC3II expressions were significantly increased. CONCLUSIONS The current study has shown that the exercise-induced increased LSS enhances the level of autophagy-related protein expressions in vascular endothelial cells.http://www.ksep-es.org/upload/pdf/es-27-2-140.pdfaerobic exerciseshear stressautophagyendothelial cellsvasculature |
| spellingShingle | Ji-Seok Kim Kijeong Kim Hojun Lee Effects of Aerobic Exercise on the Autophagy-Related Protein Expressions in Vascular Endothelial Cells 운동과학 aerobic exercise shear stress autophagy endothelial cells vasculature |
| title | Effects of Aerobic Exercise on the Autophagy-Related Protein Expressions in Vascular Endothelial Cells |
| title_full | Effects of Aerobic Exercise on the Autophagy-Related Protein Expressions in Vascular Endothelial Cells |
| title_fullStr | Effects of Aerobic Exercise on the Autophagy-Related Protein Expressions in Vascular Endothelial Cells |
| title_full_unstemmed | Effects of Aerobic Exercise on the Autophagy-Related Protein Expressions in Vascular Endothelial Cells |
| title_short | Effects of Aerobic Exercise on the Autophagy-Related Protein Expressions in Vascular Endothelial Cells |
| title_sort | effects of aerobic exercise on the autophagy related protein expressions in vascular endothelial cells |
| topic | aerobic exercise shear stress autophagy endothelial cells vasculature |
| url | http://www.ksep-es.org/upload/pdf/es-27-2-140.pdf |
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