Combining Celery Oleoresin, Limonene and Rhamnolipid as New Strategy to Control Endospore-Forming <em>Bacillus cereus</em>
Foodborne diseases (FBD) are a great problem worldwide, leading millions of people to seek medical help and to significant economic losses for industry. Among the agents implicated in FDB is <i>Bacillus cereus</i>, a Gram-positive, toxigenic and endospore-forming bacterium. In this study...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/10/2/455 |
| _version_ | 1797395990436642816 |
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| author | Paula de Camargo Bertuso Débora M. Drappé Mayer Marcia Nitschke |
| author_facet | Paula de Camargo Bertuso Débora M. Drappé Mayer Marcia Nitschke |
| author_sort | Paula de Camargo Bertuso |
| collection | DOAJ |
| description | Foodborne diseases (FBD) are a great problem worldwide, leading millions of people to seek medical help and to significant economic losses for industry. Among the agents implicated in FDB is <i>Bacillus cereus</i>, a Gram-positive, toxigenic and endospore-forming bacterium. In this study, rhamnolipid (RL) biosurfactant, celery oleoresin (OR) and limonene (LN) were evaluated as bio-based alternatives for controlling the growth of vegetative cells and endospores of <i>B. cereus</i>. To address their antimicrobial activity, the compounds were tested separately and in combination. Results demonstrate that, when combined with RL, both OR and LN have lower minimal inhibitory concentration (MIC) values and increased endospore inhibition potential. A percentage of endospore inhibition from 73% to 98%, corresponding to a 2.8–3.6 log reduction in spore outgrowth, was observed. RL inhibited <i>B. cereus</i> growth and endospore germination and potentially enhanced the antimicrobial efficacy of the natural hydrophobic compounds tested. |
| first_indexed | 2024-03-09T00:44:47Z |
| format | Article |
| id | doaj.art-89455a1421914943a7837ed07b329f5c |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T00:44:47Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-89455a1421914943a7837ed07b329f5c2023-12-11T17:37:49ZengMDPI AGFoods2304-81582021-02-0110245510.3390/foods10020455Combining Celery Oleoresin, Limonene and Rhamnolipid as New Strategy to Control Endospore-Forming <em>Bacillus cereus</em>Paula de Camargo Bertuso0Débora M. Drappé Mayer1Marcia Nitschke2Interunits Graduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo, Trabalhador São-Carlense Av., 400, São Carlos, SP 13566-590, BrazilInterunits Graduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo, Trabalhador São-Carlense Av., 400, São Carlos, SP 13566-590, BrazilSão Carlos Institute of Chemistry (IQSC), University of São Paulo, Trabalhador São-Carlense Av., 400, P.O. Box 780, São Carlos, SP 13560-970, BrazilFoodborne diseases (FBD) are a great problem worldwide, leading millions of people to seek medical help and to significant economic losses for industry. Among the agents implicated in FDB is <i>Bacillus cereus</i>, a Gram-positive, toxigenic and endospore-forming bacterium. In this study, rhamnolipid (RL) biosurfactant, celery oleoresin (OR) and limonene (LN) were evaluated as bio-based alternatives for controlling the growth of vegetative cells and endospores of <i>B. cereus</i>. To address their antimicrobial activity, the compounds were tested separately and in combination. Results demonstrate that, when combined with RL, both OR and LN have lower minimal inhibitory concentration (MIC) values and increased endospore inhibition potential. A percentage of endospore inhibition from 73% to 98%, corresponding to a 2.8–3.6 log reduction in spore outgrowth, was observed. RL inhibited <i>B. cereus</i> growth and endospore germination and potentially enhanced the antimicrobial efficacy of the natural hydrophobic compounds tested.https://www.mdpi.com/2304-8158/10/2/455<i>Bacillus cereus</i>rhamnolipid<i>Apium graveolens</i>oleoresinlimonene |
| spellingShingle | Paula de Camargo Bertuso Débora M. Drappé Mayer Marcia Nitschke Combining Celery Oleoresin, Limonene and Rhamnolipid as New Strategy to Control Endospore-Forming <em>Bacillus cereus</em> Foods <i>Bacillus cereus</i> rhamnolipid <i>Apium graveolens</i> oleoresin limonene |
| title | Combining Celery Oleoresin, Limonene and Rhamnolipid as New Strategy to Control Endospore-Forming <em>Bacillus cereus</em> |
| title_full | Combining Celery Oleoresin, Limonene and Rhamnolipid as New Strategy to Control Endospore-Forming <em>Bacillus cereus</em> |
| title_fullStr | Combining Celery Oleoresin, Limonene and Rhamnolipid as New Strategy to Control Endospore-Forming <em>Bacillus cereus</em> |
| title_full_unstemmed | Combining Celery Oleoresin, Limonene and Rhamnolipid as New Strategy to Control Endospore-Forming <em>Bacillus cereus</em> |
| title_short | Combining Celery Oleoresin, Limonene and Rhamnolipid as New Strategy to Control Endospore-Forming <em>Bacillus cereus</em> |
| title_sort | combining celery oleoresin limonene and rhamnolipid as new strategy to control endospore forming em bacillus cereus em |
| topic | <i>Bacillus cereus</i> rhamnolipid <i>Apium graveolens</i> oleoresin limonene |
| url | https://www.mdpi.com/2304-8158/10/2/455 |
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