Variability in indigenous Pakistani tomato lines and worldwide reference collection for Tomato Mosaic Virus (ToMV) and Tomato Yellow Leaf Curl Virus (TYLCV) infection
Abstract Local and exotic germplasm of tomato remains a major source for genetic improvement. Assessment of such lines for biotic stresses particularly viral diseases are the most important criteria for selection in Pakistan, where Tomato Yellow Leaf Curl Virus (TYLCV) and Tomato Mosaic Virus (ToMV)...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2022-02-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100193&tlng=en |
| _version_ | 1818490203983052800 |
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| author | I. Hussain T. Farooq S. A. Khan N. Ali M. Waris A. Jalal S. L. Nielsen S. Ali |
| author_facet | I. Hussain T. Farooq S. A. Khan N. Ali M. Waris A. Jalal S. L. Nielsen S. Ali |
| author_sort | I. Hussain |
| collection | DOAJ |
| description | Abstract Local and exotic germplasm of tomato remains a major source for genetic improvement. Assessment of such lines for biotic stresses particularly viral diseases are the most important criteria for selection in Pakistan, where Tomato Yellow Leaf Curl Virus (TYLCV) and Tomato Mosaic Virus (ToMV) are the major diseases/viruses. A set of 40 accessions (including indigenous Pakistani lines and exotic germplasm from Europe, the United States, and Asia) were evaluated for their resistance/infection response to ToMV with artificial inoculation under greenhouse conditions. Infection response was quantified through disease scoring and DAS-ELISA test (for ToMV). A subset of 24 lines, was further screened for TYLCV using disease scoring and TAS-ELISA. The tested lines showed significant variability for resistance to ToMV. Only one accession (Acc-17878) was resistant to the ToMV whereas seven accessions i.e. Acc-17890, AVR-261, CLN-312, AVR-321, EUR-333, CLN-352, and CLN-362 expressed resistance to TYLCV. Correlation between phenotypic evaluation was confirmed by the ELISA results in both diseases, although both tools complemented to assess the viral infection status. In future, tomato breeding programs must consider breeding for ToMV and TYLCV resistance (using identified germplasm in our study) so as to deliver virus resistant tomato varieties. |
| first_indexed | 2024-12-10T17:14:10Z |
| format | Article |
| id | doaj.art-89466e1f24aa49349defbac0be68c04e |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-10T17:14:10Z |
| publishDate | 2022-02-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-89466e1f24aa49349defbac0be68c04e2022-12-22T01:40:12ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752022-02-018410.1590/1519-6984.253605Variability in indigenous Pakistani tomato lines and worldwide reference collection for Tomato Mosaic Virus (ToMV) and Tomato Yellow Leaf Curl Virus (TYLCV) infectionI. Hussainhttps://orcid.org/0000-0002-7999-3871T. Farooqhttps://orcid.org/0000-0001-7738-7993S. A. Khanhttps://orcid.org/0000-0002-6662-4786N. Alihttps://orcid.org/0000-0001-8118-3678M. Warishttps://orcid.org/0000-0002-6107-704XA. Jalalhttps://orcid.org/0000-0002-0218-5171S. L. NielsenS. Alihttps://orcid.org/0000-0003-3915-7390Abstract Local and exotic germplasm of tomato remains a major source for genetic improvement. Assessment of such lines for biotic stresses particularly viral diseases are the most important criteria for selection in Pakistan, where Tomato Yellow Leaf Curl Virus (TYLCV) and Tomato Mosaic Virus (ToMV) are the major diseases/viruses. A set of 40 accessions (including indigenous Pakistani lines and exotic germplasm from Europe, the United States, and Asia) were evaluated for their resistance/infection response to ToMV with artificial inoculation under greenhouse conditions. Infection response was quantified through disease scoring and DAS-ELISA test (for ToMV). A subset of 24 lines, was further screened for TYLCV using disease scoring and TAS-ELISA. The tested lines showed significant variability for resistance to ToMV. Only one accession (Acc-17878) was resistant to the ToMV whereas seven accessions i.e. Acc-17890, AVR-261, CLN-312, AVR-321, EUR-333, CLN-352, and CLN-362 expressed resistance to TYLCV. Correlation between phenotypic evaluation was confirmed by the ELISA results in both diseases, although both tools complemented to assess the viral infection status. In future, tomato breeding programs must consider breeding for ToMV and TYLCV resistance (using identified germplasm in our study) so as to deliver virus resistant tomato varieties.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100193&tlng=enLycopersicon esculentumELISAartificial inoculationdisease severityindigenous and exotic lines |
| spellingShingle | I. Hussain T. Farooq S. A. Khan N. Ali M. Waris A. Jalal S. L. Nielsen S. Ali Variability in indigenous Pakistani tomato lines and worldwide reference collection for Tomato Mosaic Virus (ToMV) and Tomato Yellow Leaf Curl Virus (TYLCV) infection Brazilian Journal of Biology Lycopersicon esculentum ELISA artificial inoculation disease severity indigenous and exotic lines |
| title | Variability in indigenous Pakistani tomato lines and worldwide reference collection for Tomato Mosaic Virus (ToMV) and Tomato Yellow Leaf Curl Virus (TYLCV) infection |
| title_full | Variability in indigenous Pakistani tomato lines and worldwide reference collection for Tomato Mosaic Virus (ToMV) and Tomato Yellow Leaf Curl Virus (TYLCV) infection |
| title_fullStr | Variability in indigenous Pakistani tomato lines and worldwide reference collection for Tomato Mosaic Virus (ToMV) and Tomato Yellow Leaf Curl Virus (TYLCV) infection |
| title_full_unstemmed | Variability in indigenous Pakistani tomato lines and worldwide reference collection for Tomato Mosaic Virus (ToMV) and Tomato Yellow Leaf Curl Virus (TYLCV) infection |
| title_short | Variability in indigenous Pakistani tomato lines and worldwide reference collection for Tomato Mosaic Virus (ToMV) and Tomato Yellow Leaf Curl Virus (TYLCV) infection |
| title_sort | variability in indigenous pakistani tomato lines and worldwide reference collection for tomato mosaic virus tomv and tomato yellow leaf curl virus tylcv infection |
| topic | Lycopersicon esculentum ELISA artificial inoculation disease severity indigenous and exotic lines |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000100193&tlng=en |
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