Analysis of TLR2, TLR4, and TLR9 single nucleotide polymorphisms in children with bacterial meningitis and their healthy family members
Background: The aim was to analyse TLR2 rs5743708, TLR2 rs4696480, TLR4 rs4986790, TLR9 rs5743836, and TLR9 rs352140 single nucleotide polymorphisms (SNPs) in children with pneumococcal and meningococcal meningitis and their family members. Methods: The study group consisted of 39 children with bact...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-07-01
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| Series: | International Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971217301364 |
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| author | Ewelina Gowin Bogna Świątek-Kościelna Ewelina Kałużna Jerzy Nowak Michał Michalak Jacek Wysocki Danuta Januszkiewicz-Lewandowska |
| author_facet | Ewelina Gowin Bogna Świątek-Kościelna Ewelina Kałużna Jerzy Nowak Michał Michalak Jacek Wysocki Danuta Januszkiewicz-Lewandowska |
| author_sort | Ewelina Gowin |
| collection | DOAJ |
| description | Background: The aim was to analyse TLR2 rs5743708, TLR2 rs4696480, TLR4 rs4986790, TLR9 rs5743836, and TLR9 rs352140 single nucleotide polymorphisms (SNPs) in children with pneumococcal and meningococcal meningitis and their family members.
Methods: The study group consisted of 39 children with bacterial meningitis (25 with meningococcal meningitis and 14 with pneumococcal meningitis) and 49 family members. Laboratory test results and the course of the diseases were analyzed. Genomic DNA was extracted from 1.2 ml of peripheral blood in order to analyze the five SNPs.
Results: Patients with pneumococcal and meningococcal meningitis showed a similar male/female ratio, mean age, and duration of symptoms. There were no statistically significant differences in biochemical markers between the two groups. All patients possessed at least one polymorphic variant of the analyzed SNPs. The most common SNP was TLR9 rs352140, detected in 89.7% of patients. No significant differences in SNP frequency were found between patients, family members, and the general population.
Conclusions: The allele frequencies in the population studied are in accordance with the literature data. The study did not find an association between the analyzed SNPs and susceptibility to bacterial meningitis. The role of SNPs in genes coding toll-like receptors and the interactions between them in controlling inflammation in the central nervous system needs further evaluation. |
| first_indexed | 2024-12-16T18:24:22Z |
| format | Article |
| id | doaj.art-8946e71b833d44bebed45a798a493436 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 1878-3511 |
| language | English |
| last_indexed | 2024-12-16T18:24:22Z |
| publishDate | 2017-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-8946e71b833d44bebed45a798a4934362022-12-21T22:21:27ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112017-07-0160C232810.1016/j.ijid.2017.04.024Analysis of TLR2, TLR4, and TLR9 single nucleotide polymorphisms in children with bacterial meningitis and their healthy family membersEwelina Gowin0Bogna Świątek-Kościelna1Ewelina Kałużna2Jerzy Nowak3Michał Michalak4Jacek Wysocki5Danuta Januszkiewicz-Lewandowska6Department of Family Medicine, Poznań University of Medical Sciences, Przybyszewskiego 39, 60-355 Poznań, PolandDepartment of Molecular Pathology, Institute of Human Genetics, Polish Academy of Sciences, Poznań, PolandDepartment of Molecular Pathology, Institute of Human Genetics, Polish Academy of Sciences, Poznań, PolandDepartment of Molecular Pathology, Institute of Human Genetics, Polish Academy of Sciences, Poznań, PolandDepartment of Biostatistics, Poznań University of Medical Sciences, Poznań, PolandDepartment of Health Promotion, Poznań University of Medical Sciences, Poznań, PolandDepartment of Molecular Pathology, Institute of Human Genetics, Polish Academy of Sciences, Poznań, PolandBackground: The aim was to analyse TLR2 rs5743708, TLR2 rs4696480, TLR4 rs4986790, TLR9 rs5743836, and TLR9 rs352140 single nucleotide polymorphisms (SNPs) in children with pneumococcal and meningococcal meningitis and their family members. Methods: The study group consisted of 39 children with bacterial meningitis (25 with meningococcal meningitis and 14 with pneumococcal meningitis) and 49 family members. Laboratory test results and the course of the diseases were analyzed. Genomic DNA was extracted from 1.2 ml of peripheral blood in order to analyze the five SNPs. Results: Patients with pneumococcal and meningococcal meningitis showed a similar male/female ratio, mean age, and duration of symptoms. There were no statistically significant differences in biochemical markers between the two groups. All patients possessed at least one polymorphic variant of the analyzed SNPs. The most common SNP was TLR9 rs352140, detected in 89.7% of patients. No significant differences in SNP frequency were found between patients, family members, and the general population. Conclusions: The allele frequencies in the population studied are in accordance with the literature data. The study did not find an association between the analyzed SNPs and susceptibility to bacterial meningitis. The role of SNPs in genes coding toll-like receptors and the interactions between them in controlling inflammation in the central nervous system needs further evaluation.http://www.sciencedirect.com/science/article/pii/S1201971217301364MeningitisSingle nucleotide polymorphismToll-like receptor |
| spellingShingle | Ewelina Gowin Bogna Świątek-Kościelna Ewelina Kałużna Jerzy Nowak Michał Michalak Jacek Wysocki Danuta Januszkiewicz-Lewandowska Analysis of TLR2, TLR4, and TLR9 single nucleotide polymorphisms in children with bacterial meningitis and their healthy family members International Journal of Infectious Diseases Meningitis Single nucleotide polymorphism Toll-like receptor |
| title | Analysis of TLR2, TLR4, and TLR9 single nucleotide polymorphisms in children with bacterial meningitis and their healthy family members |
| title_full | Analysis of TLR2, TLR4, and TLR9 single nucleotide polymorphisms in children with bacterial meningitis and their healthy family members |
| title_fullStr | Analysis of TLR2, TLR4, and TLR9 single nucleotide polymorphisms in children with bacterial meningitis and their healthy family members |
| title_full_unstemmed | Analysis of TLR2, TLR4, and TLR9 single nucleotide polymorphisms in children with bacterial meningitis and their healthy family members |
| title_short | Analysis of TLR2, TLR4, and TLR9 single nucleotide polymorphisms in children with bacterial meningitis and their healthy family members |
| title_sort | analysis of tlr2 tlr4 and tlr9 single nucleotide polymorphisms in children with bacterial meningitis and their healthy family members |
| topic | Meningitis Single nucleotide polymorphism Toll-like receptor |
| url | http://www.sciencedirect.com/science/article/pii/S1201971217301364 |
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