Protocol for Comprehensive Synthetic Lethality Screens
Summary: Here, we provide a detailed protocol for synthetic lethality screens in a Jurkat T cell leukemia line using cell death as the readout measuring the combinatorial effect of a pan-PI3K inhibitor (GDC0941) with specific gene depletion by shRNA. We describe the use of an ultra-complex shRNA lib...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-06-01
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| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720300034 |
| _version_ | 1811295953930420224 |
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| author | Damia Romero-Moya Jeroen P. Roose |
| author_facet | Damia Romero-Moya Jeroen P. Roose |
| author_sort | Damia Romero-Moya |
| collection | DOAJ |
| description | Summary: Here, we provide a detailed protocol for synthetic lethality screens in a Jurkat T cell leukemia line using cell death as the readout measuring the combinatorial effect of a pan-PI3K inhibitor (GDC0941) with specific gene depletion by shRNA. We describe the use of an ultra-complex shRNA library, coverage considerations, time frames, protocol details, and bottlenecks with images to facilitate similar approaches. We discuss how this protocol resource can be readily adapted by investigators.For complete details on the use and execution of this protocol, please refer to (Mues et al., 2019). |
| first_indexed | 2024-04-13T05:41:12Z |
| format | Article |
| id | doaj.art-8946ed008ceb44ebb369d7c07becd595 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T05:41:12Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-8946ed008ceb44ebb369d7c07becd5952022-12-22T03:00:06ZengElsevierSTAR Protocols2666-16672020-06-0111100016Protocol for Comprehensive Synthetic Lethality ScreensDamia Romero-Moya0Jeroen P. Roose1Department of Anatomy, University of California San Francisco (UCSF), 513 Parnassus Avenue, Room HSW-1326, San Francisco, CA 94143-0452, USADepartment of Anatomy, University of California San Francisco (UCSF), 513 Parnassus Avenue, Room HSW-1326, San Francisco, CA 94143-0452, USA; Corresponding authorSummary: Here, we provide a detailed protocol for synthetic lethality screens in a Jurkat T cell leukemia line using cell death as the readout measuring the combinatorial effect of a pan-PI3K inhibitor (GDC0941) with specific gene depletion by shRNA. We describe the use of an ultra-complex shRNA library, coverage considerations, time frames, protocol details, and bottlenecks with images to facilitate similar approaches. We discuss how this protocol resource can be readily adapted by investigators.For complete details on the use and execution of this protocol, please refer to (Mues et al., 2019).http://www.sciencedirect.com/science/article/pii/S2666166720300034 |
| spellingShingle | Damia Romero-Moya Jeroen P. Roose Protocol for Comprehensive Synthetic Lethality Screens STAR Protocols |
| title | Protocol for Comprehensive Synthetic Lethality Screens |
| title_full | Protocol for Comprehensive Synthetic Lethality Screens |
| title_fullStr | Protocol for Comprehensive Synthetic Lethality Screens |
| title_full_unstemmed | Protocol for Comprehensive Synthetic Lethality Screens |
| title_short | Protocol for Comprehensive Synthetic Lethality Screens |
| title_sort | protocol for comprehensive synthetic lethality screens |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720300034 |
| work_keys_str_mv | AT damiaromeromoya protocolforcomprehensivesyntheticlethalityscreens AT jeroenproose protocolforcomprehensivesyntheticlethalityscreens |