Protocol for Comprehensive Synthetic Lethality Screens

Protocol for Comprehensive Synthetic Lethality Screens

Summary: Here, we provide a detailed protocol for synthetic lethality screens in a Jurkat T cell leukemia line using cell death as the readout measuring the combinatorial effect of a pan-PI3K inhibitor (GDC0941) with specific gene depletion by shRNA. We describe the use of an ultra-complex shRNA lib...

Full description

Bibliographic Details
Main Authors:	Damia Romero-Moya, Jeroen P. Roose
Format:	Article
Language:	English
Published:	Elsevier 2020-06-01
Series:	STAR Protocols
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166720300034

Similar Items

High-Complexity shRNA Libraries and PI3 Kinase Inhibition in Cancer: High-Fidelity Synthetic Lethality Predictions
by: Marsilius Mues, et al.
Published: (2019-04-01)

CRISPR Screens in Synthetic Lethality and Combinatorial Therapies for Cancer
by: Laia Castells-Roca, et al.
Published: (2021-03-01)

Target expression is a relevant factor in synthetic lethal screens
by: Iman J. Schultz, et al.
Published: (2022-08-01)

Reply to: Target expression is a relevant factor in synthetic lethal screens
by: Yosi Gilad, et al.
Published: (2022-08-01)

Synthetic lethal vulnerabilities of cancer.
by: Fece de la Cruz, F, et al.
Published: (2015)

Synthetic lethality: general principles, utility and detection using genetic screens in human cells.
by: Nijman, S
Published: (2011)

Discovery of synthetic lethal interactions from large-scale pan-cancer perturbation screens
by: Sumana Srivatsa, et al.
Published: (2022-12-01)

Cancer. Potential of the synthetic lethality principle.
by: Nijman, S, et al.
Published: (2013)

Overview of Ferroptosis and Synthetic Lethality Strategies
by: Yuko Kinowaki, et al.
Published: (2021-08-01)

Synthetic Lethality Targeting Polθ
by: Małgorzata Drzewiecka, et al.
Published: (2022-06-01)

Genome-wide CRISPR screen identifies genes synthetically lethal with GRA17, a nutrient channel encoding gene in Toxoplasma.
by: Tatiana C Paredes-Santos, et al.
Published: (2023-07-01)

Genetic screening for anticancer genes highlights FBLN5 as a synthetic lethal partner of MYC
by: Motasim Masood, et al.
Published: (2023-10-01)

Novel Treatments of Uveal Melanoma Identified with a Synthetic Lethal CRISPR/Cas9 Screen
by: Kseniya Glinkina, et al.
Published: (2022-06-01)

Synthetic Lethality Approaches in Acute Lymphoblastic Leukemia
by: Francisco Alejandro Lagunas-Rangel, et al.
Published: (2023-12-01)

Breast Cancer Predisposition Genes and Synthetic Lethality
by: Hannah E. Neiger, et al.
Published: (2021-05-01)

Harnessing Synthetic Lethal Interactions for Personalized Medicine
by: Grace S. Shieh
Published: (2022-01-01)

Paralog-based synthetic lethality: rationales and applications
by: Yucui Xin, et al.
Published: (2023-06-01)

Mslar: Microbial synthetic lethal and rescue database.
by: Sen-Bin Zhu, et al.
Published: (2023-06-01)

Synthetic lethality between PAXX and XLF in mammalian development
by: Balmus, G, et al.
Published: (2016)

Kinome‐Wide Synthetic Lethal Screen Identifies PANK4 as a Modulator of Temozolomide Resistance in Glioblastoma
by: Viviana Vella, et al.
Published: (2024-04-01)

Synthetic Lethality Screening Highlights Colorectal Cancer Vulnerability to Concomitant Blockade of NEDD8 and EGFR Pathways
by: Federica Invrea, et al.
Published: (2021-07-01)

Genetic landscape of T cells identifies synthetic lethality for T-ALL
by: Connor P. O’Meara, et al.
Published: (2021-10-01)

Syn-lethality : an integrative knowledge base of synthetic lethality towards discovery of selective anticancer therapies
by: Li, Xue-juan, et al.
Published: (2014)

Filling in the gaps in PARP inhibitor-induced synthetic lethality
by: Mariana Paes Dias, et al.
Published: (2021-11-01)

Synthetic lethality between TP53 and ENDOD1
by: Zizhi Tang, et al.
Published: (2022-05-01)

Exploiting synthetic lethal interactions for targeted cancer therapy
by: Reinhardt, H. Christian, et al.
Published: (2012)

In Silico Screening Identifies a Novel Potential PARP1 Inhibitor Targeting Synthetic Lethality in Cancer Treatment
by: Jian Li, et al.
Published: (2016-02-01)

Synthetic Lethal Screen Identifies NF-κB as a Target for Combination Therapy with Topotecan for patients with Neuroblastoma
by: Tsang Patricia S, et al.
Published: (2012-03-01)

Synthetic lethal screening in the mammalian central nervous system identifies Gpx6 as a modulator of Huntington’s disease
by: Kulicke, Ruth, et al.
Published: (2015)

Haem oxygenase is synthetically lethal with the tumour suppressor fumarate hydratase.
by: Frezza, C, et al.
Published: (2011)

Haem oxygenase is synthetically lethal with the tumour suppressor fumarate hydratase
by: Frezza, C, et al.
Published: (2011)

Cancer Metabolism as a Therapeutic Target: Metabolic Synthetic Lethality.
by: Bensaad, K, et al.
Published: (2013)

Small-molecule inhibitors of the HIF pathway and synthetic lethal interactions.
by: Jones, D, et al.
Published: (2012)

The power and the promise of synthetic lethality for clinical application in cancer treatment
by: Qian-Wen Liu, et al.
Published: (2024-03-01)

Niacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma
by: Miyuki Nomura, et al.
Published: (2023-12-01)

The Implication of Topoisomerase II Inhibitors in Synthetic Lethality for Cancer Therapy
by: Victor M. Matias-Barrios, et al.
Published: (2023-01-01)

Synthetic lethal approaches to target cancers with loss of PTEN function
by: Ayse Ertay, et al.
Published: (2023-11-01)

Synthetic lethal gene pairs: Experimental approaches and predictive models
by: Shan Tang, et al.
Published: (2022-12-01)

Metabolic plasticity in synthetic lethal mutants: Viability at higher cost.
by: Francesco Alessandro Massucci, et al.
Published: (2018-01-01)

Using Yeast Synthetic Lethality To Inform Drug Combination for Malaria
by: Guan, Xue Li, et al.
Published: (2018)