Protocol for Comprehensive Synthetic Lethality Screens
Summary: Here, we provide a detailed protocol for synthetic lethality screens in a Jurkat T cell leukemia line using cell death as the readout measuring the combinatorial effect of a pan-PI3K inhibitor (GDC0941) with specific gene depletion by shRNA. We describe the use of an ultra-complex shRNA lib...
Main Authors: | Damia Romero-Moya, Jeroen P. Roose |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2020-06-01
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Series: | STAR Protocols |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720300034 |
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