The Composites of PCL and Tetranuclear Titanium(IV)–Oxo Complex with Acetylsalicylate Ligands—Assessment of Their Biocompatibility and Antimicrobial Activity with the Correlation to EPR Spectroscopy
In our research, we have focused on the biological studies on composite materials produced by the dispersion of titanium(IV)–oxo complex (TOC) with acetylsalicylate ligands in a poly(ε-caprolactone) (PCL) matrix, which is a biodegradable thermoplastic polymer increasingly used in the production of m...
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2022-12-01
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| author | Julia Śmigiel Piotr Piszczek Grzegorz Wrzeszcz Tomasz Jędrzejewski Patrycja Golińska Aleksandra Radtke |
| author_facet | Julia Śmigiel Piotr Piszczek Grzegorz Wrzeszcz Tomasz Jędrzejewski Patrycja Golińska Aleksandra Radtke |
| author_sort | Julia Śmigiel |
| collection | DOAJ |
| description | In our research, we have focused on the biological studies on composite materials produced by the dispersion of titanium(IV)–oxo complex (TOC) with acetylsalicylate ligands in a poly(ε-caprolactone) (PCL) matrix, which is a biodegradable thermoplastic polymer increasingly used in the production of medical devices. Using PCL as a matrix for the biologically active compounds, such as antimicrobial agents, antibiotics or other active medical substances, from which these individuals can be gradually released is fully understable. Composites of PCL + nTOC (n = 10, 15 and 20 wt.%) have been produced and, in such a form, the biological properties of TOCs have been estimated. Direct and indirect cytotoxicity studies have been performed in vitro on L929 and human umbilical vein endothelial cells (HUVEC) cell lines. The antibacterial and antifungal activity of the PCL + TOC samples have been assessed against two <i>Staphylococcus aureus</i> (ATCC 6538 and ATCC 25923) reference strains, two <i>Escherichia coli</i> (ATCC 8739 and ATCC 25922) reference strains and yeast of <i>Candida albicans</i> ATCC 10231. Obtained results have been correlated with electron paramagnetic resonance (EPR) spectroscopy data. We could conclude that photoexcitation by visible light of the surface of PCL + nTOC composite foils lead to the formation of different paramagnetic species, mainly O<sup>−</sup>, which slowly disappears over time; however, their destructive effect on bacteria and cells has been proven. |
| first_indexed | 2024-03-11T09:55:10Z |
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| id | doaj.art-89490d7e4db5437e80b621d9c4dca5a2 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-11T09:55:10Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-89490d7e4db5437e80b621d9c4dca5a22023-11-16T15:49:44ZengMDPI AGMaterials1996-19442022-12-0116129710.3390/ma16010297The Composites of PCL and Tetranuclear Titanium(IV)–Oxo Complex with Acetylsalicylate Ligands—Assessment of Their Biocompatibility and Antimicrobial Activity with the Correlation to EPR SpectroscopyJulia Śmigiel0Piotr Piszczek1Grzegorz Wrzeszcz2Tomasz Jędrzejewski3Patrycja Golińska4Aleksandra Radtke5Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, PolandFaculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, PolandFaculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, PolandFaculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, PolandFaculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, PolandFaculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, PolandIn our research, we have focused on the biological studies on composite materials produced by the dispersion of titanium(IV)–oxo complex (TOC) with acetylsalicylate ligands in a poly(ε-caprolactone) (PCL) matrix, which is a biodegradable thermoplastic polymer increasingly used in the production of medical devices. Using PCL as a matrix for the biologically active compounds, such as antimicrobial agents, antibiotics or other active medical substances, from which these individuals can be gradually released is fully understable. Composites of PCL + nTOC (n = 10, 15 and 20 wt.%) have been produced and, in such a form, the biological properties of TOCs have been estimated. Direct and indirect cytotoxicity studies have been performed in vitro on L929 and human umbilical vein endothelial cells (HUVEC) cell lines. The antibacterial and antifungal activity of the PCL + TOC samples have been assessed against two <i>Staphylococcus aureus</i> (ATCC 6538 and ATCC 25923) reference strains, two <i>Escherichia coli</i> (ATCC 8739 and ATCC 25922) reference strains and yeast of <i>Candida albicans</i> ATCC 10231. Obtained results have been correlated with electron paramagnetic resonance (EPR) spectroscopy data. We could conclude that photoexcitation by visible light of the surface of PCL + nTOC composite foils lead to the formation of different paramagnetic species, mainly O<sup>−</sup>, which slowly disappears over time; however, their destructive effect on bacteria and cells has been proven.https://www.mdpi.com/1996-1944/16/1/297titanium(IV) complexoxo-complexcompositeantimicrobial activitycytotoxicitypoly(ε-caprolactone) |
| spellingShingle | Julia Śmigiel Piotr Piszczek Grzegorz Wrzeszcz Tomasz Jędrzejewski Patrycja Golińska Aleksandra Radtke The Composites of PCL and Tetranuclear Titanium(IV)–Oxo Complex with Acetylsalicylate Ligands—Assessment of Their Biocompatibility and Antimicrobial Activity with the Correlation to EPR Spectroscopy Materials titanium(IV) complex oxo-complex composite antimicrobial activity cytotoxicity poly(ε-caprolactone) |
| title | The Composites of PCL and Tetranuclear Titanium(IV)–Oxo Complex with Acetylsalicylate Ligands—Assessment of Their Biocompatibility and Antimicrobial Activity with the Correlation to EPR Spectroscopy |
| title_full | The Composites of PCL and Tetranuclear Titanium(IV)–Oxo Complex with Acetylsalicylate Ligands—Assessment of Their Biocompatibility and Antimicrobial Activity with the Correlation to EPR Spectroscopy |
| title_fullStr | The Composites of PCL and Tetranuclear Titanium(IV)–Oxo Complex with Acetylsalicylate Ligands—Assessment of Their Biocompatibility and Antimicrobial Activity with the Correlation to EPR Spectroscopy |
| title_full_unstemmed | The Composites of PCL and Tetranuclear Titanium(IV)–Oxo Complex with Acetylsalicylate Ligands—Assessment of Their Biocompatibility and Antimicrobial Activity with the Correlation to EPR Spectroscopy |
| title_short | The Composites of PCL and Tetranuclear Titanium(IV)–Oxo Complex with Acetylsalicylate Ligands—Assessment of Their Biocompatibility and Antimicrobial Activity with the Correlation to EPR Spectroscopy |
| title_sort | composites of pcl and tetranuclear titanium iv oxo complex with acetylsalicylate ligands assessment of their biocompatibility and antimicrobial activity with the correlation to epr spectroscopy |
| topic | titanium(IV) complex oxo-complex composite antimicrobial activity cytotoxicity poly(ε-caprolactone) |
| url | https://www.mdpi.com/1996-1944/16/1/297 |
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