Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro
Aptazyme and CRISPR/Cas gene editing system were widely used for regulating gene expression in various diseases, including cancer. This work aimed to reconstruct CRISPR/Cas13d tool for sensing hTERT exclusively based on the new device OFF-switch hTERT aptazyme that was inserted into the 3’ UTR of th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.646412/full |
| _version_ | 1818576884809596928 |
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| author | Chengle Zhuang Changshui Zhuang Qun Zhou Xueting Huang Yaoting Gui Yongqing Lai Shangqi Yang |
| author_facet | Chengle Zhuang Changshui Zhuang Qun Zhou Xueting Huang Yaoting Gui Yongqing Lai Shangqi Yang |
| author_sort | Chengle Zhuang |
| collection | DOAJ |
| description | Aptazyme and CRISPR/Cas gene editing system were widely used for regulating gene expression in various diseases, including cancer. This work aimed to reconstruct CRISPR/Cas13d tool for sensing hTERT exclusively based on the new device OFF-switch hTERT aptazyme that was inserted into the 3’ UTR of the Cas13d. In bladder cancer cells, hTERT ligand bound to aptamer in OFF-switch hTERT aptazyme to inhibit the degradation of Cas13d. Results showed that engineered CRISPR/Cas13d sensing hTERT suppressed cell proliferation, migration, invasion and induced cell apoptosis in bladder cancer 5637 and T24 cells without affecting normal HFF cells. In short, we constructed engineered CRISPR/Cas13d sensing hTERT selectively inhibited the progression of bladder cancer cells significantly. It may serve as a promising specifically effective therapy for bladder cancer cells. |
| first_indexed | 2024-12-16T06:21:07Z |
| format | Article |
| id | doaj.art-8949f81758be4ffbbf899cbd7dec7aed |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-16T06:21:07Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-8949f81758be4ffbbf899cbd7dec7aed2022-12-21T22:41:08ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-03-01810.3389/fmolb.2021.646412646412Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In VitroChengle Zhuang0Changshui Zhuang1Qun Zhou2Xueting Huang3Yaoting Gui4Yongqing Lai5Shangqi Yang6Department of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Urology, Union Shenzhen Hospital, Huazhong University of Science and Technology, Shenzhen, ChinaDepartment of Urology, the Affiliated Nanhua Hospital of University of South China, Hengyang, ChinaDepartment of Nephrorheumatology, Shenzhen Yantian District People’s Hospital, Shenzhen, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaAptazyme and CRISPR/Cas gene editing system were widely used for regulating gene expression in various diseases, including cancer. This work aimed to reconstruct CRISPR/Cas13d tool for sensing hTERT exclusively based on the new device OFF-switch hTERT aptazyme that was inserted into the 3’ UTR of the Cas13d. In bladder cancer cells, hTERT ligand bound to aptamer in OFF-switch hTERT aptazyme to inhibit the degradation of Cas13d. Results showed that engineered CRISPR/Cas13d sensing hTERT suppressed cell proliferation, migration, invasion and induced cell apoptosis in bladder cancer 5637 and T24 cells without affecting normal HFF cells. In short, we constructed engineered CRISPR/Cas13d sensing hTERT selectively inhibited the progression of bladder cancer cells significantly. It may serve as a promising specifically effective therapy for bladder cancer cells.https://www.frontiersin.org/articles/10.3389/fmolb.2021.646412/fullCRISPR/Cas13dhTERTbladder canceraptazymedegradation |
| spellingShingle | Chengle Zhuang Changshui Zhuang Qun Zhou Xueting Huang Yaoting Gui Yongqing Lai Shangqi Yang Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro Frontiers in Molecular Biosciences CRISPR/Cas13d hTERT bladder cancer aptazyme degradation |
| title | Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro |
| title_full | Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro |
| title_fullStr | Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro |
| title_full_unstemmed | Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro |
| title_short | Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro |
| title_sort | engineered crispr cas13d sensing htert selectively inhibits the progression of bladder cancer in vitro |
| topic | CRISPR/Cas13d hTERT bladder cancer aptazyme degradation |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.646412/full |
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