Flazin as a Lipid Droplet Regulator against Lipid Disorders
Lipid disorders are closely related to numerous metabolic diseases, and lipid droplets (LDs) have been considered as a new target for regulating lipid metabolism. Dietary intervention and nutraceuticals provide safe and long-term beneficial effects for treating metabolic diseases. Flazin is a diet-d...
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MDPI AG
2022-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/7/1501 |
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| author | Xunzhi Wu Zhen Chen Yue Wu Yifan Chen Jiaping Jia Nianqiu Shen Hitoshi Chiba Shu-Ping Hui |
| author_facet | Xunzhi Wu Zhen Chen Yue Wu Yifan Chen Jiaping Jia Nianqiu Shen Hitoshi Chiba Shu-Ping Hui |
| author_sort | Xunzhi Wu |
| collection | DOAJ |
| description | Lipid disorders are closely related to numerous metabolic diseases, and lipid droplets (LDs) have been considered as a new target for regulating lipid metabolism. Dietary intervention and nutraceuticals provide safe and long-term beneficial effects for treating metabolic diseases. Flazin is a diet-derived bioactive constituent mainly existing in fermented foods, of which the lipid metabolism improvement function has not been studied. In this study, the effect of flazin on lipid regulation at both cell level and organelle level was investigated. Lipidomic profiling showed that flazin significantly decreased cellular triglyceride (TG) by 12.0–22.4% compared with modeling groups and improved the TG and free fatty acid profile. LD staining revealed that flazin efficiently reduced both cellular neutral lipid content by 17.4–53.9% and LD size by 10.0–35.3%. Furthermore, nanoelectrospray ionization mass spectrometry analysis proved that flazin exhibited a preferential suppression of LD TG and regulated LD morphology, including a size decrease and surface property improvement. An evaluation of related gene expression suggested the mechanism to be lipolysis promotion and lipogenesis inhibition. These findings indicated that flazin might be an LD regulator for reversing lipid metabolism disturbance. Moreover, the strategy proposed in this study may contribute to developing other nutraceuticals for treating lipid disorder-related metabolic diseases. |
| first_indexed | 2024-03-09T11:32:43Z |
| format | Article |
| id | doaj.art-894b94c9f3ca48b59849d4848fcf0e7f |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T11:32:43Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-894b94c9f3ca48b59849d4848fcf0e7f2023-11-30T23:49:09ZengMDPI AGNutrients2072-66432022-04-01147150110.3390/nu14071501Flazin as a Lipid Droplet Regulator against Lipid DisordersXunzhi Wu0Zhen Chen1Yue Wu2Yifan Chen3Jiaping Jia4Nianqiu Shen5Hitoshi Chiba6Shu-Ping Hui7Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanDepartment of Nutrition, Sapporo University of Health Sciences, Nakanuma Nishi-4-2-1-15, Higashi-ku, Sapporo 007-0894, JapanFaculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-ku, Sapporo 060-0812, JapanLipid disorders are closely related to numerous metabolic diseases, and lipid droplets (LDs) have been considered as a new target for regulating lipid metabolism. Dietary intervention and nutraceuticals provide safe and long-term beneficial effects for treating metabolic diseases. Flazin is a diet-derived bioactive constituent mainly existing in fermented foods, of which the lipid metabolism improvement function has not been studied. In this study, the effect of flazin on lipid regulation at both cell level and organelle level was investigated. Lipidomic profiling showed that flazin significantly decreased cellular triglyceride (TG) by 12.0–22.4% compared with modeling groups and improved the TG and free fatty acid profile. LD staining revealed that flazin efficiently reduced both cellular neutral lipid content by 17.4–53.9% and LD size by 10.0–35.3%. Furthermore, nanoelectrospray ionization mass spectrometry analysis proved that flazin exhibited a preferential suppression of LD TG and regulated LD morphology, including a size decrease and surface property improvement. An evaluation of related gene expression suggested the mechanism to be lipolysis promotion and lipogenesis inhibition. These findings indicated that flazin might be an LD regulator for reversing lipid metabolism disturbance. Moreover, the strategy proposed in this study may contribute to developing other nutraceuticals for treating lipid disorder-related metabolic diseases.https://www.mdpi.com/2072-6643/14/7/1501lipid-storage disordersmetabolic diseasesdiabetic nephropathynutraceuticalsfunctional foodstriglyceride |
| spellingShingle | Xunzhi Wu Zhen Chen Yue Wu Yifan Chen Jiaping Jia Nianqiu Shen Hitoshi Chiba Shu-Ping Hui Flazin as a Lipid Droplet Regulator against Lipid Disorders Nutrients lipid-storage disorders metabolic diseases diabetic nephropathy nutraceuticals functional foods triglyceride |
| title | Flazin as a Lipid Droplet Regulator against Lipid Disorders |
| title_full | Flazin as a Lipid Droplet Regulator against Lipid Disorders |
| title_fullStr | Flazin as a Lipid Droplet Regulator against Lipid Disorders |
| title_full_unstemmed | Flazin as a Lipid Droplet Regulator against Lipid Disorders |
| title_short | Flazin as a Lipid Droplet Regulator against Lipid Disorders |
| title_sort | flazin as a lipid droplet regulator against lipid disorders |
| topic | lipid-storage disorders metabolic diseases diabetic nephropathy nutraceuticals functional foods triglyceride |
| url | https://www.mdpi.com/2072-6643/14/7/1501 |
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