Effects of Thawing Temperature of Frozen rare Breed Hanwoo (Korean Native Cattle) Semen on Viability of Refrozen Spermatozoa
Cryopreservation of germ cells from genetically proven animals could be a source of restoration tools from the risk of extinction or disappearance of wanted characteristics. Using frozen semen, the genetic gains of Korean native cattle have been increased greatly for 70 years. The preservation of ge...
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The Korean Society of Animal Reproduction and Biotechnology
2017-03-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | http://www.e-jarb.org/journal/view.html?uid=11&vmd=Full |
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| author | Min Su Kim Arum Choi Chan-Lan Kim Dongkyo Kim Hwan-Hoo Seong Sung Woo Kim |
| author_facet | Min Su Kim Arum Choi Chan-Lan Kim Dongkyo Kim Hwan-Hoo Seong Sung Woo Kim |
| author_sort | Min Su Kim |
| collection | DOAJ |
| description | Cryopreservation of germ cells from genetically proven animals could be a source of restoration tools from the risk of extinction or disappearance of wanted characteristics. Using frozen semen, the genetic gains of Korean native cattle have been increased greatly for 70 years. The preservation of genetic resources as a form of frozen semen straw has limited availability due to the numbers. To circumvent this weakness of frozen semen, we tested two re-freezing methods with different initial thawing temperatures using frozen Korean proven semen and rare breed semen from albino, black and chikso breeders. It has been known that human sperm could resist to cryo-damages by repeated freeze-thaw cycles, but not for Korean proven bulls number (KPN) or for rare breeds. Total 7 frozen semem from brindled(2), black(1), Korean Albino(2) and KPN(1) bulls were used for our research. After thawing straws under 5°C/2min or 37°C/40sec with low temperature water bath and thermo jug, spermatozoa were re-diluted with triladyl diluents after first thawing and re-frozen. Sperm motilities were compared between animals and treated groups after re-thawing. Mean values of motility and viability of refrozen/thawed sperm for expansion of the number of straws were significantly higher in 5°C than in 37°C (P< < 0.05). However, the activity of viable sperm thawed at 5°C was significantly decreased after first and second thawing. It is estimated that re-freezing of frozen semen from rare Korean native cattle is possible with resistant properties of survived spermatozoa. |
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| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-17T00:29:04Z |
| publishDate | 2017-03-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
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| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-894b97bb32c540d49dca82a86edd5a812022-12-21T22:10:23ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632017-03-013211810.12750/JET.2017.32.1.1Effects of Thawing Temperature of Frozen rare Breed Hanwoo (Korean Native Cattle) Semen on Viability of Refrozen SpermatozoaMin Su Kim0Arum Choi1Chan-Lan Kim2 Dongkyo Kim3Hwan-Hoo Seong4Sung Woo Kim5Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaCryopreservation of germ cells from genetically proven animals could be a source of restoration tools from the risk of extinction or disappearance of wanted characteristics. Using frozen semen, the genetic gains of Korean native cattle have been increased greatly for 70 years. The preservation of genetic resources as a form of frozen semen straw has limited availability due to the numbers. To circumvent this weakness of frozen semen, we tested two re-freezing methods with different initial thawing temperatures using frozen Korean proven semen and rare breed semen from albino, black and chikso breeders. It has been known that human sperm could resist to cryo-damages by repeated freeze-thaw cycles, but not for Korean proven bulls number (KPN) or for rare breeds. Total 7 frozen semem from brindled(2), black(1), Korean Albino(2) and KPN(1) bulls were used for our research. After thawing straws under 5°C/2min or 37°C/40sec with low temperature water bath and thermo jug, spermatozoa were re-diluted with triladyl diluents after first thawing and re-frozen. Sperm motilities were compared between animals and treated groups after re-thawing. Mean values of motility and viability of refrozen/thawed sperm for expansion of the number of straws were significantly higher in 5°C than in 37°C (P< < 0.05). However, the activity of viable sperm thawed at 5°C was significantly decreased after first and second thawing. It is estimated that re-freezing of frozen semen from rare Korean native cattle is possible with resistant properties of survived spermatozoa.http://www.e-jarb.org/journal/view.html?uid=11&vmd=Fullsemenfreezingrefreezinggenetic materials |
| spellingShingle | Min Su Kim Arum Choi Chan-Lan Kim Dongkyo Kim Hwan-Hoo Seong Sung Woo Kim Effects of Thawing Temperature of Frozen rare Breed Hanwoo (Korean Native Cattle) Semen on Viability of Refrozen Spermatozoa Journal of Animal Reproduction and Biotechnology semen freezing refreezing genetic materials |
| title | Effects of Thawing Temperature of Frozen rare Breed Hanwoo (Korean Native Cattle) Semen on Viability of Refrozen Spermatozoa |
| title_full | Effects of Thawing Temperature of Frozen rare Breed Hanwoo (Korean Native Cattle) Semen on Viability of Refrozen Spermatozoa |
| title_fullStr | Effects of Thawing Temperature of Frozen rare Breed Hanwoo (Korean Native Cattle) Semen on Viability of Refrozen Spermatozoa |
| title_full_unstemmed | Effects of Thawing Temperature of Frozen rare Breed Hanwoo (Korean Native Cattle) Semen on Viability of Refrozen Spermatozoa |
| title_short | Effects of Thawing Temperature of Frozen rare Breed Hanwoo (Korean Native Cattle) Semen on Viability of Refrozen Spermatozoa |
| title_sort | effects of thawing temperature of frozen rare breed hanwoo korean native cattle semen on viability of refrozen spermatozoa |
| topic | semen freezing refreezing genetic materials |
| url | http://www.e-jarb.org/journal/view.html?uid=11&vmd=Full |
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