Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an ongoing coronavirus disease (COVID-19) outbreak and a rising demand for the development of accurate, timely, and cost-effective diagnostic tests for SARS-CoV-2 as well as other viral infections in general. Currently, traditio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/13/4/490 |
| _version_ | 1827745698505818112 |
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| author | Kieu The Loan Trinh Hoang Dang Khoa Do Nae Yoon Lee |
| author_facet | Kieu The Loan Trinh Hoang Dang Khoa Do Nae Yoon Lee |
| author_sort | Kieu The Loan Trinh |
| collection | DOAJ |
| description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an ongoing coronavirus disease (COVID-19) outbreak and a rising demand for the development of accurate, timely, and cost-effective diagnostic tests for SARS-CoV-2 as well as other viral infections in general. Currently, traditional virus screening methods such as plate culturing and real-time PCR are considered the gold standard with accurate and sensitive results. However, these methods still require sophisticated equipment, trained personnel, and a long analysis time. Alternatively, with the integration of microfluidic and biosensor technologies, microfluidic-based biosensors offer the ability to perform sample preparation and simultaneous detection of many analyses in one platform. High sensitivity, accuracy, portability, low cost, high throughput, and real-time detection can be achieved using a single platform. This review presents recent advances in microfluidic-based biosensors from many works to demonstrate the advantages of merging the two technologies for sensing viruses. Different platforms for virus detection are classified into two main sections: immunoassays and molecular assays. Moreover, available commercial sensing tests are analyzed. |
| first_indexed | 2024-03-11T05:12:07Z |
| format | Article |
| id | doaj.art-894d5409b60d445f808ce488f3a67db9 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T05:12:07Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-894d5409b60d445f808ce488f3a67db92023-11-17T18:31:25ZengMDPI AGBiosensors2079-63742023-04-0113449010.3390/bios13040490Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A ReviewKieu The Loan Trinh0Hoang Dang Khoa Do1Nae Yoon Lee2Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 13120, Gyeonggi-do, Republic of KoreaNTT Hi-Tech Institute, Nguyen Tat Thanh University, Ward 13, District 04, Ho Chi Minh City 70000, VietnamDepartment of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 13120, Gyeonggi-do, Republic of KoreaSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an ongoing coronavirus disease (COVID-19) outbreak and a rising demand for the development of accurate, timely, and cost-effective diagnostic tests for SARS-CoV-2 as well as other viral infections in general. Currently, traditional virus screening methods such as plate culturing and real-time PCR are considered the gold standard with accurate and sensitive results. However, these methods still require sophisticated equipment, trained personnel, and a long analysis time. Alternatively, with the integration of microfluidic and biosensor technologies, microfluidic-based biosensors offer the ability to perform sample preparation and simultaneous detection of many analyses in one platform. High sensitivity, accuracy, portability, low cost, high throughput, and real-time detection can be achieved using a single platform. This review presents recent advances in microfluidic-based biosensors from many works to demonstrate the advantages of merging the two technologies for sensing viruses. Different platforms for virus detection are classified into two main sections: immunoassays and molecular assays. Moreover, available commercial sensing tests are analyzed.https://www.mdpi.com/2079-6374/13/4/490microfluidicsvirusimmunoassaymolecular assay |
| spellingShingle | Kieu The Loan Trinh Hoang Dang Khoa Do Nae Yoon Lee Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review Biosensors microfluidics virus immunoassay molecular assay |
| title | Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review |
| title_full | Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review |
| title_fullStr | Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review |
| title_full_unstemmed | Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review |
| title_short | Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review |
| title_sort | recent advances in molecular and immunological diagnostic platform for virus detection a review |
| topic | microfluidics virus immunoassay molecular assay |
| url | https://www.mdpi.com/2079-6374/13/4/490 |
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