Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis
Although RAD51 associated protein 1 (RAD51AP1) is crucial in genome stability maintenance, it also promotes cancer development with an unclear mechanism. In this study, we collected intact expression data of RAD51AP1 from the public database, and verified it was significantly over-expressed in 33 ca...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.971033/full |
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| author | Renwang Liu Renwang Liu Guangsheng Zhu Guangsheng Zhu Mingbiao Li Peijun Cao Peijun Cao Xuanguang Li Xuanguang Li Xiuwen Zhang Xiuwen Zhang Hua Huang Hua Huang Zuoqing Song Zuoqing Song Jun Chen Jun Chen |
| author_facet | Renwang Liu Renwang Liu Guangsheng Zhu Guangsheng Zhu Mingbiao Li Peijun Cao Peijun Cao Xuanguang Li Xuanguang Li Xiuwen Zhang Xiuwen Zhang Hua Huang Hua Huang Zuoqing Song Zuoqing Song Jun Chen Jun Chen |
| author_sort | Renwang Liu |
| collection | DOAJ |
| description | Although RAD51 associated protein 1 (RAD51AP1) is crucial in genome stability maintenance, it also promotes cancer development with an unclear mechanism. In this study, we collected intact expression data of RAD51AP1 from the public database, and verified it was significantly over-expressed in 33 cancer types and correlated with poor prognosis in 13 cancer types, including glioma, adrenocortical carcinoma, lung adenocarcinoma. We further authenticated that RAD51AP1 is up-regulated in several typical cancer cell lines and promotes cancer cell proliferation in vitro. Moreover, we also demonstrated that RAD51AP1 was significantly positively related to cancer stemness score mRNAsi in 27 cancer types and broadly correlated to tumor-infiltrating immune cells in various cancers in a diverse manner. It was also negatively associated with immunophenoscore (IPS) and Estimation of STromal and Immune cells in MAlignant Tumours using Expression data (ESTIMATE) scores and positively correlated with mutant-allele tumor heterogeneity (MATH), tumor mutational burden (TMB), microsatellite instability (MSI), and PD-L1 expression in multiple cancers. The tumor stemness enhancing and tumor immune microenvironment affecting functions of RAD51AP1 might compose its carcinogenesis mechanism. Further investigations beyond the bioinformatics level should confirm these findings in each specific cancer. |
| first_indexed | 2024-04-11T16:31:33Z |
| format | Article |
| id | doaj.art-894e0d3752644aa28186d522c53bf387 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T16:31:33Z |
| publishDate | 2022-11-01 |
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| series | Frontiers in Genetics |
| spelling | doaj.art-894e0d3752644aa28186d522c53bf3872022-12-22T04:14:01ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-11-011310.3389/fgene.2022.971033971033Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosisRenwang Liu0Renwang Liu1Guangsheng Zhu2Guangsheng Zhu3Mingbiao Li4Peijun Cao5Peijun Cao6Xuanguang Li7Xuanguang Li8Xiuwen Zhang9Xiuwen Zhang10Hua Huang11Hua Huang12Zuoqing Song13Zuoqing Song14Jun Chen15Jun Chen16Department of Lung Cancer Surgery, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Lung Cancer Metastasis and Tumour Microenvironment, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Lung Cancer Surgery, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Lung Cancer Metastasis and Tumour Microenvironment, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Lung Cancer Metastasis and Tumour Microenvironment, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Lung Cancer Surgery, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Lung Cancer Metastasis and Tumour Microenvironment, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Lung Cancer Surgery, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Lung Cancer Metastasis and Tumour Microenvironment, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Lung Cancer Surgery, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Lung Cancer Metastasis and Tumour Microenvironment, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Lung Cancer Surgery, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Lung Cancer Metastasis and Tumour Microenvironment, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Lung Cancer Surgery, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Lung Cancer Metastasis and Tumour Microenvironment, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Lung Cancer Surgery, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaTianjin Key Laboratory of Lung Cancer Metastasis and Tumour Microenvironment, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, ChinaAlthough RAD51 associated protein 1 (RAD51AP1) is crucial in genome stability maintenance, it also promotes cancer development with an unclear mechanism. In this study, we collected intact expression data of RAD51AP1 from the public database, and verified it was significantly over-expressed in 33 cancer types and correlated with poor prognosis in 13 cancer types, including glioma, adrenocortical carcinoma, lung adenocarcinoma. We further authenticated that RAD51AP1 is up-regulated in several typical cancer cell lines and promotes cancer cell proliferation in vitro. Moreover, we also demonstrated that RAD51AP1 was significantly positively related to cancer stemness score mRNAsi in 27 cancer types and broadly correlated to tumor-infiltrating immune cells in various cancers in a diverse manner. It was also negatively associated with immunophenoscore (IPS) and Estimation of STromal and Immune cells in MAlignant Tumours using Expression data (ESTIMATE) scores and positively correlated with mutant-allele tumor heterogeneity (MATH), tumor mutational burden (TMB), microsatellite instability (MSI), and PD-L1 expression in multiple cancers. The tumor stemness enhancing and tumor immune microenvironment affecting functions of RAD51AP1 might compose its carcinogenesis mechanism. Further investigations beyond the bioinformatics level should confirm these findings in each specific cancer.https://www.frontiersin.org/articles/10.3389/fgene.2022.971033/fullRAD51AP1carcinogenesistumor immune microenvironmentpan-cancerbioinformatics analysis |
| spellingShingle | Renwang Liu Renwang Liu Guangsheng Zhu Guangsheng Zhu Mingbiao Li Peijun Cao Peijun Cao Xuanguang Li Xuanguang Li Xiuwen Zhang Xiuwen Zhang Hua Huang Hua Huang Zuoqing Song Zuoqing Song Jun Chen Jun Chen Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis Frontiers in Genetics RAD51AP1 carcinogenesis tumor immune microenvironment pan-cancer bioinformatics analysis |
| title | Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis |
| title_full | Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis |
| title_fullStr | Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis |
| title_full_unstemmed | Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis |
| title_short | Systematic pan-cancer analysis showed that RAD51AP1 was associated with immune microenvironment, tumor stemness, and prognosis |
| title_sort | systematic pan cancer analysis showed that rad51ap1 was associated with immune microenvironment tumor stemness and prognosis |
| topic | RAD51AP1 carcinogenesis tumor immune microenvironment pan-cancer bioinformatics analysis |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.971033/full |
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