Time dependency of morphological remodeling of endothelial cells in response to substrate stiffness
Introduction: Substrate stiffness regulates cellular behavior as cells experience different stiffness values of tissues in the body. For example, endothelial cells (ECs) covering the inner layer of blood vessels are exposed to different stiffness values due to various pathologic and physiologic cond...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2017-02-01
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| Series: | BioImpacts |
| Subjects: | |
| Online Access: | http://bi.tbzmed.ac.ir/PDF/bi-7-41.pdf |
| _version_ | 1817981961434562560 |
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| author | Zahra Goli-Malekabadi Mohammad Tafazzoli-shadpour Ali Tamayol Ehsan Seyedjafari |
| author_facet | Zahra Goli-Malekabadi Mohammad Tafazzoli-shadpour Ali Tamayol Ehsan Seyedjafari |
| author_sort | Zahra Goli-Malekabadi |
| collection | DOAJ |
| description | Introduction: Substrate stiffness regulates cellular behavior as cells experience different stiffness values of tissues in the body. For example, endothelial cells (ECs) covering the inner layer of blood vessels are exposed to different stiffness values due to various pathologic and physiologic conditions. Despite numerous studies, cells by time span sense mechanical properties of the substrate, but the response is not well understood. We hypothesized that time is a major determinant influencing the behavior of cells seeded on substrates of varying stiffness.
Methods: We monitored cell spreading, internal structure, 3D topography, and the viability of ECs over 24 hours of culture on polydimethylsiloxane (PDMS) substrates with two different degrees of elastic modulus.
Results: Despite significant differences in cell spreading after cell seeding, cells showed a similar shape and internal structure after 24 hours of culture on both soft and stiff substrates. However, 3D topographical images confirmed existence of rich lamellipodia and filopodia around the cells cultured on stiffer PDMS substrates.
Conclusion: It was concluded that the response of ECs to the substrate stiffness was time dependent with initial enhanced cellular spreading and viability on stiffer substrates. Results can provide a better comprehension of cell mechanotransduction for tissue engineering applications. |
| first_indexed | 2024-04-13T23:14:09Z |
| format | Article |
| id | doaj.art-894e2649ac794bf8bf3f38dc4697d49c |
| institution | Directory Open Access Journal |
| issn | 2228-5660 2228-5652 |
| language | English |
| last_indexed | 2024-04-13T23:14:09Z |
| publishDate | 2017-02-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | BioImpacts |
| spelling | doaj.art-894e2649ac794bf8bf3f38dc4697d49c2022-12-22T02:25:28ZengTabriz University of Medical SciencesBioImpacts2228-56602228-56522017-02-0171414710.15171/bi.2017.06BI_14701_20161002012357Time dependency of morphological remodeling of endothelial cells in response to substrate stiffnessZahra Goli-Malekabadi0Mohammad Tafazzoli-shadpour1Ali Tamayol2Ehsan Seyedjafari3Biomedical Engineering Department, Amirkabir University of Technology, Tehran, IranBiomedical Engineering Department, Amirkabir University of Technology, Tehran, IranDepartment of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, USADepartment of Biotechnology, College of Science, University of Tehran, Tehran, IranIntroduction: Substrate stiffness regulates cellular behavior as cells experience different stiffness values of tissues in the body. For example, endothelial cells (ECs) covering the inner layer of blood vessels are exposed to different stiffness values due to various pathologic and physiologic conditions. Despite numerous studies, cells by time span sense mechanical properties of the substrate, but the response is not well understood. We hypothesized that time is a major determinant influencing the behavior of cells seeded on substrates of varying stiffness. Methods: We monitored cell spreading, internal structure, 3D topography, and the viability of ECs over 24 hours of culture on polydimethylsiloxane (PDMS) substrates with two different degrees of elastic modulus. Results: Despite significant differences in cell spreading after cell seeding, cells showed a similar shape and internal structure after 24 hours of culture on both soft and stiff substrates. However, 3D topographical images confirmed existence of rich lamellipodia and filopodia around the cells cultured on stiffer PDMS substrates. Conclusion: It was concluded that the response of ECs to the substrate stiffness was time dependent with initial enhanced cellular spreading and viability on stiffer substrates. Results can provide a better comprehension of cell mechanotransduction for tissue engineering applications.http://bi.tbzmed.ac.ir/PDF/bi-7-41.pdfTime dependencyHUVECsSubstrate stiffnessMorphology3D topography |
| spellingShingle | Zahra Goli-Malekabadi Mohammad Tafazzoli-shadpour Ali Tamayol Ehsan Seyedjafari Time dependency of morphological remodeling of endothelial cells in response to substrate stiffness BioImpacts Time dependency HUVECs Substrate stiffness Morphology 3D topography |
| title | Time dependency of morphological remodeling of endothelial cells in response to substrate stiffness |
| title_full | Time dependency of morphological remodeling of endothelial cells in response to substrate stiffness |
| title_fullStr | Time dependency of morphological remodeling of endothelial cells in response to substrate stiffness |
| title_full_unstemmed | Time dependency of morphological remodeling of endothelial cells in response to substrate stiffness |
| title_short | Time dependency of morphological remodeling of endothelial cells in response to substrate stiffness |
| title_sort | time dependency of morphological remodeling of endothelial cells in response to substrate stiffness |
| topic | Time dependency HUVECs Substrate stiffness Morphology 3D topography |
| url | http://bi.tbzmed.ac.ir/PDF/bi-7-41.pdf |
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