Representation of the Hyrcanian forest (northern Iran) in modern pollen rain revealed by palynological and DNA-metabarcoding data
We studied the modern pollen rain in two different landscapes from Hyrcanian lowland forests up to the slopes of the Alborz Mountains in Gilan province for the first time. Pollen traps were installed for one year and moss samples were collected along two altitudinal transects from 100 to 1800 m and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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W. Szafer Institute of Botany, Polish Academy of Sciences
2023-06-01
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| Series: | Acta Palaeobotanica |
| Subjects: | |
| Online Access: | https://acpa.botany.pl/Representation-of-the-Hyrcanian-forest-northern-Iran-in-modern-pollen-rain-revealed,162167,0,2.html |
| _version_ | 1797751317010055168 |
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| author | Leila Homami Totmaj Arash Rasi Katrin Neumann Sepideh Pirouzi Kammaledin Alizadeh Hermann Behling |
| author_facet | Leila Homami Totmaj Arash Rasi Katrin Neumann Sepideh Pirouzi Kammaledin Alizadeh Hermann Behling |
| author_sort | Leila Homami Totmaj |
| collection | DOAJ |
| description | We studied the modern pollen rain in two different landscapes from Hyrcanian lowland forests up
to the slopes of the Alborz Mountains in Gilan province for the first time. Pollen traps were installed for one year
and moss samples were collected along two altitudinal transects from 100 to 1800 m and from 100 to 2300 m
elevations. The results of pollen counting and environmental DNA barcoding (metabarcoding) of the collected
pollen and moss samples were compared from 32 locations. In total, 81 vascular plant species from 36 families
were identified by metabarcoding, and 68 taxa belonging to 39 families were identified by pollen counting. The
pollen counting results reflect mainly wind-pollinated families, such as Betulaceae and Fagaceae while results
from metabarcoding of the rbcL and ITS2 loci were more in line with the vegetation around the pollen traps and
the moss samples. Furthermore, this study showed that the rbcL region is able to identify more taxa than the
ITS2 region, while applying both markers provides higher confidence. Also using both metabarcoding and pollen
data provides a better local and regional vegetation representation. |
| first_indexed | 2024-03-12T16:45:39Z |
| format | Article |
| id | doaj.art-894e5f7c081f4fc5ae27456a20955f32 |
| institution | Directory Open Access Journal |
| issn | 2082-0259 |
| language | English |
| last_indexed | 2024-03-12T16:45:39Z |
| publishDate | 2023-06-01 |
| publisher | W. Szafer Institute of Botany, Polish Academy of Sciences |
| record_format | Article |
| series | Acta Palaeobotanica |
| spelling | doaj.art-894e5f7c081f4fc5ae27456a20955f322023-08-08T13:02:57ZengW. Szafer Institute of Botany, Polish Academy of SciencesActa Palaeobotanica2082-02592023-06-0163110011710.35535/acpa-2023-0007162167Representation of the Hyrcanian forest (northern Iran) in modern pollen rain revealed by palynological and DNA-metabarcoding dataLeila Homami Totmaj0https://orcid.org/0000-0003-4852-9811Arash Rasi1Katrin Neumann2Sepideh Pirouzi3Kammaledin Alizadeh4Hermann Behling5University of Göttingen, Department of Palynology and Climate Dynamics, Albrecht-von-Haller Institute for Plant Sciences, Untere Karspüle 2, 37073 Göttingen, GermanyUniversity of Tehran, Department of Agronomy and Plant Breeding, Agricultural and Natural Resources College, Division of Plant Biotechnology, Alborz, IranLifeprint GmbH, Industriestrasse 12, 89257, Illertissen, GermanyUniversity of Tehran, Department of Plant Science, Center of Excellence in Phylogeny, School of Biology, College of Science, Tehran, IranQuality Service International GmbH, Department of Product Management, Flughafendamm 9a, 28199, Bremen, GermanyUniversity of Göttingen, Department of Palynology and Climate Dynamics, Albrecht-von-Haller Institute for Plant Sciences, Untere Karspüle 2, 37073 Göttingen, GermanyWe studied the modern pollen rain in two different landscapes from Hyrcanian lowland forests up to the slopes of the Alborz Mountains in Gilan province for the first time. Pollen traps were installed for one year and moss samples were collected along two altitudinal transects from 100 to 1800 m and from 100 to 2300 m elevations. The results of pollen counting and environmental DNA barcoding (metabarcoding) of the collected pollen and moss samples were compared from 32 locations. In total, 81 vascular plant species from 36 families were identified by metabarcoding, and 68 taxa belonging to 39 families were identified by pollen counting. The pollen counting results reflect mainly wind-pollinated families, such as Betulaceae and Fagaceae while results from metabarcoding of the rbcL and ITS2 loci were more in line with the vegetation around the pollen traps and the moss samples. Furthermore, this study showed that the rbcL region is able to identify more taxa than the ITS2 region, while applying both markers provides higher confidence. Also using both metabarcoding and pollen data provides a better local and regional vegetation representation.https://acpa.botany.pl/Representation-of-the-Hyrcanian-forest-northern-Iran-in-modern-pollen-rain-revealed,162167,0,2.htmlhyrcanian forestmodern pollen rainmetabarcodingits2rbclpalynology |
| spellingShingle | Leila Homami Totmaj Arash Rasi Katrin Neumann Sepideh Pirouzi Kammaledin Alizadeh Hermann Behling Representation of the Hyrcanian forest (northern Iran) in modern pollen rain revealed by palynological and DNA-metabarcoding data Acta Palaeobotanica hyrcanian forest modern pollen rain metabarcoding its2 rbcl palynology |
| title | Representation of the Hyrcanian forest (northern Iran) in modern pollen rain revealed by palynological and DNA-metabarcoding data |
| title_full | Representation of the Hyrcanian forest (northern Iran) in modern pollen rain revealed by palynological and DNA-metabarcoding data |
| title_fullStr | Representation of the Hyrcanian forest (northern Iran) in modern pollen rain revealed by palynological and DNA-metabarcoding data |
| title_full_unstemmed | Representation of the Hyrcanian forest (northern Iran) in modern pollen rain revealed by palynological and DNA-metabarcoding data |
| title_short | Representation of the Hyrcanian forest (northern Iran) in modern pollen rain revealed by palynological and DNA-metabarcoding data |
| title_sort | representation of the hyrcanian forest northern iran in modern pollen rain revealed by palynological and dna metabarcoding data |
| topic | hyrcanian forest modern pollen rain metabarcoding its2 rbcl palynology |
| url | https://acpa.botany.pl/Representation-of-the-Hyrcanian-forest-northern-Iran-in-modern-pollen-rain-revealed,162167,0,2.html |
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