Water pH, not soil pH, alters bacterial community structural pattern and nitrogen cycling pathways in date palm (Phoenix dactylifera L.) roots and bulk soil under freshwater irrigation regime
Date palms are widely cultivated in arid agroecosystems, where knowledge of irrigation water effect on their soil and root-associated bacterial communities is limited. Using 16S rDNA metabarcoding, we studied soil and root-associated bacterial communities of date palms growing in United Arab Emirate...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fevo.2023.1142073/full |
| _version_ | 1797803334726320128 |
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| author | Dinesh Sanka Loganathachetti Sunil Mundra Sunil Mundra Sunil Mundra |
| author_facet | Dinesh Sanka Loganathachetti Sunil Mundra Sunil Mundra Sunil Mundra |
| author_sort | Dinesh Sanka Loganathachetti |
| collection | DOAJ |
| description | Date palms are widely cultivated in arid agroecosystems, where knowledge of irrigation water effect on their soil and root-associated bacterial communities is limited. Using 16S rDNA metabarcoding, we studied soil and root-associated bacterial communities of date palms growing in United Arab Emirates. Overall, 12.18% of bacterial operational taxonomic units (OTUs) were unique to roots, whereas 41.55% were specific to soil. The bacterial diversity was lower in root and community patterns were distinct between compartments, wherein irrigation water pH was a key structuring factor in both compartments, while salinity (electrical conductivity) was important only in the soil. Co-occurrence network analysis revealed a decrease in complexity in the soil–root continuum, and specific taxa/modules also varied with water pH. We observed a higher abundance of endophyte–saprotroph (Bacillus, Streptomyces and Dongia) dual-role OTUs in both compartments, possibly involved in nutrient mobilisation and plant growth. Based on PICRUSt and trait-based analyses, we showed that these OTUs are putatively involved in the nitrogen cycle (nitrification, denitrification, and assimilatory nitrate reduction). Taken together, we suggest that irrigation water pH, not soil pH, transiently affects belowground bacterial communities and selects bacteria in specific pH ranges, which may be important for nutrient cycling (i.e. nitrogen cycle) in arid agroecosystems. |
| first_indexed | 2024-03-13T05:20:14Z |
| format | Article |
| id | doaj.art-895d6436e8fe47b7a55138ccba758fad |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-03-13T05:20:14Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-895d6436e8fe47b7a55138ccba758fad2023-06-15T13:59:15ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2023-06-011110.3389/fevo.2023.11420731142073Water pH, not soil pH, alters bacterial community structural pattern and nitrogen cycling pathways in date palm (Phoenix dactylifera L.) roots and bulk soil under freshwater irrigation regimeDinesh Sanka Loganathachetti0Sunil Mundra1Sunil Mundra2Sunil Mundra3Department of Biology, College of Science, United Arab Emirates University, Al-Ain, Abu-Dhabi, United Arab EmiratesDepartment of Biology, College of Science, United Arab Emirates University, Al-Ain, Abu-Dhabi, United Arab EmiratesKhalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al Ain, United Arab EmiratesNational Water and Energy Center, United Arab Emirates University, Al Ain, United Arab EmiratesDate palms are widely cultivated in arid agroecosystems, where knowledge of irrigation water effect on their soil and root-associated bacterial communities is limited. Using 16S rDNA metabarcoding, we studied soil and root-associated bacterial communities of date palms growing in United Arab Emirates. Overall, 12.18% of bacterial operational taxonomic units (OTUs) were unique to roots, whereas 41.55% were specific to soil. The bacterial diversity was lower in root and community patterns were distinct between compartments, wherein irrigation water pH was a key structuring factor in both compartments, while salinity (electrical conductivity) was important only in the soil. Co-occurrence network analysis revealed a decrease in complexity in the soil–root continuum, and specific taxa/modules also varied with water pH. We observed a higher abundance of endophyte–saprotroph (Bacillus, Streptomyces and Dongia) dual-role OTUs in both compartments, possibly involved in nutrient mobilisation and plant growth. Based on PICRUSt and trait-based analyses, we showed that these OTUs are putatively involved in the nitrogen cycle (nitrification, denitrification, and assimilatory nitrate reduction). Taken together, we suggest that irrigation water pH, not soil pH, transiently affects belowground bacterial communities and selects bacteria in specific pH ranges, which may be important for nutrient cycling (i.e. nitrogen cycle) in arid agroecosystems.https://www.frontiersin.org/articles/10.3389/fevo.2023.1142073/fullarid agroecosystemsdate palms (Phoenix dactylifera L.)irrigation waternitrogen cycleroot and soil bacterial communityco-occurrence network |
| spellingShingle | Dinesh Sanka Loganathachetti Sunil Mundra Sunil Mundra Sunil Mundra Water pH, not soil pH, alters bacterial community structural pattern and nitrogen cycling pathways in date palm (Phoenix dactylifera L.) roots and bulk soil under freshwater irrigation regime Frontiers in Ecology and Evolution arid agroecosystems date palms (Phoenix dactylifera L.) irrigation water nitrogen cycle root and soil bacterial community co-occurrence network |
| title | Water pH, not soil pH, alters bacterial community structural pattern and nitrogen cycling pathways in date palm (Phoenix dactylifera L.) roots and bulk soil under freshwater irrigation regime |
| title_full | Water pH, not soil pH, alters bacterial community structural pattern and nitrogen cycling pathways in date palm (Phoenix dactylifera L.) roots and bulk soil under freshwater irrigation regime |
| title_fullStr | Water pH, not soil pH, alters bacterial community structural pattern and nitrogen cycling pathways in date palm (Phoenix dactylifera L.) roots and bulk soil under freshwater irrigation regime |
| title_full_unstemmed | Water pH, not soil pH, alters bacterial community structural pattern and nitrogen cycling pathways in date palm (Phoenix dactylifera L.) roots and bulk soil under freshwater irrigation regime |
| title_short | Water pH, not soil pH, alters bacterial community structural pattern and nitrogen cycling pathways in date palm (Phoenix dactylifera L.) roots and bulk soil under freshwater irrigation regime |
| title_sort | water ph not soil ph alters bacterial community structural pattern and nitrogen cycling pathways in date palm phoenix dactylifera l roots and bulk soil under freshwater irrigation regime |
| topic | arid agroecosystems date palms (Phoenix dactylifera L.) irrigation water nitrogen cycle root and soil bacterial community co-occurrence network |
| url | https://www.frontiersin.org/articles/10.3389/fevo.2023.1142073/full |
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