Male contraceptive efficacy of poly herbal formulation, contracept-TM, composed of aqueous extracts of Terminalia chebula fruit and Musa balbisiana seed in rat
Context: Terminalia chebula Retz (Combretaceae) and Musa balbisiana Colla (Musaceae) have a traditional reputation as a male contraceptive. Objective: To determine the hypo-testicular activity of aqueous extracts of Terminalia chebula (fruit) and Musa balbisiana (seed) separately, and in composite m...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-01-01
|
| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2017.1357734 |
| _version_ | 1818912324205936640 |
|---|---|
| author | Abhinandan Ghosh Bhabani Prasad Pakhira Adrija Tripathy Debidas Ghosh |
| author_facet | Abhinandan Ghosh Bhabani Prasad Pakhira Adrija Tripathy Debidas Ghosh |
| author_sort | Abhinandan Ghosh |
| collection | DOAJ |
| description | Context: Terminalia chebula Retz (Combretaceae) and Musa balbisiana Colla (Musaceae) have a traditional reputation as a male contraceptive. Objective: To determine the hypo-testicular activity of aqueous extracts of Terminalia chebula (fruit) and Musa balbisiana (seed) separately, and in composite manner at the ratio of 1:1 named as ‘Contracept-TM’ compared to cyproterone acetate (CPA), for developing a polyherbal contraceptive. Materials and methods: The separate extract of above said plants or ‘Contracept-TM’ at the dose of 40 mg/100 g body weight of rat/day or CPA at 2 mg/100 g body weight of rat/day was administered for 28 days. Spermiological, androgenic and oxidative stress sensors, LD50 and ED50/100 g body weight values were measured. Results: Treatment of individual, ‘Contracept-TM’ or CPA resulted significant decrease in the count of spermatogonia A (36.36–49.09%), pre-leptotene spermatocyte (19.11–55.30%), mid-pachytene spermatocyte (28.65–47.28%) and step 7 spermatid (29.65–51.59%). Activities of testicular Δ5, 3β (21.25–48.02%),17β-hydroxysteroid dehydrogenases (29.75–55.08%), catalase (19.06–43.29%) and peroxidase (30.76–62.82%), levels of testosterone (28.15–63.44%), testicular cholesterol (19.61–49.33%), conjugated diene (29.69–84.99%) and thiobarbituric acid reactive substances (41.25–86.73%) were elevated compare to the control. The ED50 and LD50 values were 40 mg and 5.8 g (T. chebula), 48 mg and 6.3 g (M. bulbisiana), 40 mg and 6.0 g (‘Contracept-TM’), respectively. Discussion and conclusion: The said spermiological and androgenic sensors’ levels were decreased significantly by ‘Contracept-TM’ than its constitutional individual plant extract and it may be comparable to standard anti-testicular drug like CPA. So, it may be concluded that above polyherbal formulation is potent for inducing hypo-testicular activity. |
| first_indexed | 2024-12-19T23:12:47Z |
| format | Article |
| id | doaj.art-89614c8139124ea9b7aba3ab276ac1a0 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-19T23:12:47Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-89614c8139124ea9b7aba3ab276ac1a02022-12-21T20:02:10ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-015512035204210.1080/13880209.2017.13577341357734Male contraceptive efficacy of poly herbal formulation, contracept-TM, composed of aqueous extracts of Terminalia chebula fruit and Musa balbisiana seed in ratAbhinandan Ghosh0Bhabani Prasad Pakhira1Adrija Tripathy2Debidas Ghosh3Vidyasagar UniversityVidyasagar UniversityVidyasagar UniversityVidyasagar UniversityContext: Terminalia chebula Retz (Combretaceae) and Musa balbisiana Colla (Musaceae) have a traditional reputation as a male contraceptive. Objective: To determine the hypo-testicular activity of aqueous extracts of Terminalia chebula (fruit) and Musa balbisiana (seed) separately, and in composite manner at the ratio of 1:1 named as ‘Contracept-TM’ compared to cyproterone acetate (CPA), for developing a polyherbal contraceptive. Materials and methods: The separate extract of above said plants or ‘Contracept-TM’ at the dose of 40 mg/100 g body weight of rat/day or CPA at 2 mg/100 g body weight of rat/day was administered for 28 days. Spermiological, androgenic and oxidative stress sensors, LD50 and ED50/100 g body weight values were measured. Results: Treatment of individual, ‘Contracept-TM’ or CPA resulted significant decrease in the count of spermatogonia A (36.36–49.09%), pre-leptotene spermatocyte (19.11–55.30%), mid-pachytene spermatocyte (28.65–47.28%) and step 7 spermatid (29.65–51.59%). Activities of testicular Δ5, 3β (21.25–48.02%),17β-hydroxysteroid dehydrogenases (29.75–55.08%), catalase (19.06–43.29%) and peroxidase (30.76–62.82%), levels of testosterone (28.15–63.44%), testicular cholesterol (19.61–49.33%), conjugated diene (29.69–84.99%) and thiobarbituric acid reactive substances (41.25–86.73%) were elevated compare to the control. The ED50 and LD50 values were 40 mg and 5.8 g (T. chebula), 48 mg and 6.3 g (M. bulbisiana), 40 mg and 6.0 g (‘Contracept-TM’), respectively. Discussion and conclusion: The said spermiological and androgenic sensors’ levels were decreased significantly by ‘Contracept-TM’ than its constitutional individual plant extract and it may be comparable to standard anti-testicular drug like CPA. So, it may be concluded that above polyherbal formulation is potent for inducing hypo-testicular activity.http://dx.doi.org/10.1080/13880209.2017.1357734androgenesisantitesticular activitycyproterone acetateoxidative stressspermatogenesissperm motilitysperm viabilitysteroidogenesis |
| spellingShingle | Abhinandan Ghosh Bhabani Prasad Pakhira Adrija Tripathy Debidas Ghosh Male contraceptive efficacy of poly herbal formulation, contracept-TM, composed of aqueous extracts of Terminalia chebula fruit and Musa balbisiana seed in rat Pharmaceutical Biology androgenesis antitesticular activity cyproterone acetate oxidative stress spermatogenesis sperm motility sperm viability steroidogenesis |
| title | Male contraceptive efficacy of poly herbal formulation, contracept-TM, composed of aqueous extracts of Terminalia chebula fruit and Musa balbisiana seed in rat |
| title_full | Male contraceptive efficacy of poly herbal formulation, contracept-TM, composed of aqueous extracts of Terminalia chebula fruit and Musa balbisiana seed in rat |
| title_fullStr | Male contraceptive efficacy of poly herbal formulation, contracept-TM, composed of aqueous extracts of Terminalia chebula fruit and Musa balbisiana seed in rat |
| title_full_unstemmed | Male contraceptive efficacy of poly herbal formulation, contracept-TM, composed of aqueous extracts of Terminalia chebula fruit and Musa balbisiana seed in rat |
| title_short | Male contraceptive efficacy of poly herbal formulation, contracept-TM, composed of aqueous extracts of Terminalia chebula fruit and Musa balbisiana seed in rat |
| title_sort | male contraceptive efficacy of poly herbal formulation contracept tm composed of aqueous extracts of terminalia chebula fruit and musa balbisiana seed in rat |
| topic | androgenesis antitesticular activity cyproterone acetate oxidative stress spermatogenesis sperm motility sperm viability steroidogenesis |
| url | http://dx.doi.org/10.1080/13880209.2017.1357734 |
| work_keys_str_mv | AT abhinandanghosh malecontraceptiveefficacyofpolyherbalformulationcontracepttmcomposedofaqueousextractsofterminaliachebulafruitandmusabalbisianaseedinrat AT bhabaniprasadpakhira malecontraceptiveefficacyofpolyherbalformulationcontracepttmcomposedofaqueousextractsofterminaliachebulafruitandmusabalbisianaseedinrat AT adrijatripathy malecontraceptiveefficacyofpolyherbalformulationcontracepttmcomposedofaqueousextractsofterminaliachebulafruitandmusabalbisianaseedinrat AT debidasghosh malecontraceptiveefficacyofpolyherbalformulationcontracepttmcomposedofaqueousextractsofterminaliachebulafruitandmusabalbisianaseedinrat |