Robot-assisted in situ bioprinting of gelatin methacrylate hydrogels with stem cells induces hair follicle-inclusive skin regeneration
Large skin defects caused by accidents or disease can cause fluid loss, water and electrolyte disorders, hypoproteinemia and serious infection and remain a difficult problem in clinical practice. In situ bioprinting is a promising, recently developed technology that involves timely, customized, and...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
|
| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332222015293 |
| _version_ | 1811177286261538816 |
|---|---|
| author | Haiyan Chen Xiaoxiao Ma Tianya Gao Wenxiang Zhao Tao Xu Zhonghua Liu |
| author_facet | Haiyan Chen Xiaoxiao Ma Tianya Gao Wenxiang Zhao Tao Xu Zhonghua Liu |
| author_sort | Haiyan Chen |
| collection | DOAJ |
| description | Large skin defects caused by accidents or disease can cause fluid loss, water and electrolyte disorders, hypoproteinemia and serious infection and remain a difficult problem in clinical practice. In situ bioprinting is a promising, recently developed technology that involves timely, customized, and morphologically adapted bioprinting of bioink into tissue defects to promote the recovery of human tissues or organs. During this process, bioink is a key factor. In this study, we synthesized a biocompatible, photosensitive hydrogel material comprising gelatin methacrylate (GelMA) for robot-assisted in situ bioprinting of skin wounds. The results showed that GelMA demonstrated good printability of that supported the proliferation of skin-derived precursors (SKPs) and maintained their properties. Furthermore, in situ bioprinting of GelMA hydrogels with epidermal stem cells (Epi-SCs) and SKPs onto skin wounds showed complete wound healing and functional tissue skin regeneration. The regenerated skin contains epidermis, dermis, blood vessels, hair follicles, and sebaceous glands and resembling native skin. These results provide an effective strategy for skin repair through the combined application of GelMA hydrogels, Epi-SCs, SKPs and in situ bioprinting and its promising clinical translational potential for further applications. |
| first_indexed | 2024-04-10T22:59:13Z |
| format | Article |
| id | doaj.art-8961b8034bb7482e9b6ac2fb34969b53 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-10T22:59:13Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-8961b8034bb7482e9b6ac2fb34969b532023-01-14T04:25:57ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-02-01158114140Robot-assisted in situ bioprinting of gelatin methacrylate hydrogels with stem cells induces hair follicle-inclusive skin regenerationHaiyan Chen0Xiaoxiao Ma1Tianya Gao2Wenxiang Zhao3Tao Xu4Zhonghua Liu5The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, People’s Republic of China; East China Institute of Digital Medical Engineering, Shangrao 334000, People’s Republic of China; Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, People’s Republic of China; Corresponding author at: The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, People’s Republic of China.The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, People’s Republic of ChinaThe National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, People’s Republic of ChinaBiomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People’s Republic of ChinaTsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, People’s Republic of China; Bio-intelligent Manufacturing and Living Matter Bioprinting Center, Research Institute of Tsinghua University in Shenzhen, Tsinghua University, Shenzhen 518057, People’s Republic of China; Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, People’s Republic of China; Corresponding author at: The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, People’s Republic of China.The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, People’s Republic of China; Corresponding author.Large skin defects caused by accidents or disease can cause fluid loss, water and electrolyte disorders, hypoproteinemia and serious infection and remain a difficult problem in clinical practice. In situ bioprinting is a promising, recently developed technology that involves timely, customized, and morphologically adapted bioprinting of bioink into tissue defects to promote the recovery of human tissues or organs. During this process, bioink is a key factor. In this study, we synthesized a biocompatible, photosensitive hydrogel material comprising gelatin methacrylate (GelMA) for robot-assisted in situ bioprinting of skin wounds. The results showed that GelMA demonstrated good printability of that supported the proliferation of skin-derived precursors (SKPs) and maintained their properties. Furthermore, in situ bioprinting of GelMA hydrogels with epidermal stem cells (Epi-SCs) and SKPs onto skin wounds showed complete wound healing and functional tissue skin regeneration. The regenerated skin contains epidermis, dermis, blood vessels, hair follicles, and sebaceous glands and resembling native skin. These results provide an effective strategy for skin repair through the combined application of GelMA hydrogels, Epi-SCs, SKPs and in situ bioprinting and its promising clinical translational potential for further applications.http://www.sciencedirect.com/science/article/pii/S0753332222015293GelMA hydrogelsPhotosensitiveHair follicle neogenesisIn situ bioprintingEpidermal stem cellsSkin derived precursors |
| spellingShingle | Haiyan Chen Xiaoxiao Ma Tianya Gao Wenxiang Zhao Tao Xu Zhonghua Liu Robot-assisted in situ bioprinting of gelatin methacrylate hydrogels with stem cells induces hair follicle-inclusive skin regeneration Biomedicine & Pharmacotherapy GelMA hydrogels Photosensitive Hair follicle neogenesis In situ bioprinting Epidermal stem cells Skin derived precursors |
| title | Robot-assisted in situ bioprinting of gelatin methacrylate hydrogels with stem cells induces hair follicle-inclusive skin regeneration |
| title_full | Robot-assisted in situ bioprinting of gelatin methacrylate hydrogels with stem cells induces hair follicle-inclusive skin regeneration |
| title_fullStr | Robot-assisted in situ bioprinting of gelatin methacrylate hydrogels with stem cells induces hair follicle-inclusive skin regeneration |
| title_full_unstemmed | Robot-assisted in situ bioprinting of gelatin methacrylate hydrogels with stem cells induces hair follicle-inclusive skin regeneration |
| title_short | Robot-assisted in situ bioprinting of gelatin methacrylate hydrogels with stem cells induces hair follicle-inclusive skin regeneration |
| title_sort | robot assisted in situ bioprinting of gelatin methacrylate hydrogels with stem cells induces hair follicle inclusive skin regeneration |
| topic | GelMA hydrogels Photosensitive Hair follicle neogenesis In situ bioprinting Epidermal stem cells Skin derived precursors |
| url | http://www.sciencedirect.com/science/article/pii/S0753332222015293 |
| work_keys_str_mv | AT haiyanchen robotassistedinsitubioprintingofgelatinmethacrylatehydrogelswithstemcellsinduceshairfollicleinclusiveskinregeneration AT xiaoxiaoma robotassistedinsitubioprintingofgelatinmethacrylatehydrogelswithstemcellsinduceshairfollicleinclusiveskinregeneration AT tianyagao robotassistedinsitubioprintingofgelatinmethacrylatehydrogelswithstemcellsinduceshairfollicleinclusiveskinregeneration AT wenxiangzhao robotassistedinsitubioprintingofgelatinmethacrylatehydrogelswithstemcellsinduceshairfollicleinclusiveskinregeneration AT taoxu robotassistedinsitubioprintingofgelatinmethacrylatehydrogelswithstemcellsinduceshairfollicleinclusiveskinregeneration AT zhonghualiu robotassistedinsitubioprintingofgelatinmethacrylatehydrogelswithstemcellsinduceshairfollicleinclusiveskinregeneration |