Higher plant calreticulins have acquired specialized functions in Arabidopsis.
Calreticulin (CRT) is a ubiquitous ER protein involved in multiple cellular processes in animals, such as protein folding and calcium homeostasis. Like in animals, plants have evolved divergent CRTs, but their physiological functions are less understood. Arabidopsis contains three CRT proteins, wher...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-06-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2893204?pdf=render |
| _version_ | 1811321104316235776 |
|---|---|
| author | Anna Christensen Karin Svensson Lisa Thelin Wenjing Zhang Nico Tintor Daniel Prins Norma Funke Marek Michalak Paul Schulze-Lefert Yusuke Saijo Marianne Sommarin Susanne Widell Staffan Persson |
| author_facet | Anna Christensen Karin Svensson Lisa Thelin Wenjing Zhang Nico Tintor Daniel Prins Norma Funke Marek Michalak Paul Schulze-Lefert Yusuke Saijo Marianne Sommarin Susanne Widell Staffan Persson |
| author_sort | Anna Christensen |
| collection | DOAJ |
| description | Calreticulin (CRT) is a ubiquitous ER protein involved in multiple cellular processes in animals, such as protein folding and calcium homeostasis. Like in animals, plants have evolved divergent CRTs, but their physiological functions are less understood. Arabidopsis contains three CRT proteins, where the two CRTs AtCRT1a and CRT1b represent one subgroup, and AtCRT3 a divergent member.Through expression of single Arabidopsis family members in CRT-deficient mouse fibroblasts we show that both subgroups have retained basic CRT functions, including ER Ca2+-holding potential and putative chaperone capabilities. However, other more general cellular defects due to the absence of CRT in the fibroblasts, such as cell adhesion deficiencies, were not fully restored. Furthermore, in planta expression, protein localization and mutant analyses revealed that the three Arabidopsis CRTs have acquired specialized functions. The AtCRT1a and CRT1b family members appear to be components of a general ER chaperone network. In contrast, and as recently shown, AtCRT3 is associated with immune responses, and is essential for responsiveness to the bacterial Pathogen-Associated Molecular Pattern (PAMP) elf18, derived from elongation factor (EF)-Tu. Whereas constitutively expressed AtCRT1a fully complemented Atcrt1b mutants, AtCRT3 did not.We conclude that the physiological functions of the two CRT subgroups in Arabidopsis have diverged, resulting in a role for AtCRT3 in PAMP associated responses, and possibly more general chaperone functions for AtCRT1a and CRT1b. |
| first_indexed | 2024-04-13T13:10:48Z |
| format | Article |
| id | doaj.art-896628eb38b8496d8a16f6659dc1cb07 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T13:10:48Z |
| publishDate | 2010-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-896628eb38b8496d8a16f6659dc1cb072022-12-22T02:45:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-06-0156e1134210.1371/journal.pone.0011342Higher plant calreticulins have acquired specialized functions in Arabidopsis.Anna ChristensenKarin SvenssonLisa ThelinWenjing ZhangNico TintorDaniel PrinsNorma FunkeMarek MichalakPaul Schulze-LefertYusuke SaijoMarianne SommarinSusanne WidellStaffan PerssonCalreticulin (CRT) is a ubiquitous ER protein involved in multiple cellular processes in animals, such as protein folding and calcium homeostasis. Like in animals, plants have evolved divergent CRTs, but their physiological functions are less understood. Arabidopsis contains three CRT proteins, where the two CRTs AtCRT1a and CRT1b represent one subgroup, and AtCRT3 a divergent member.Through expression of single Arabidopsis family members in CRT-deficient mouse fibroblasts we show that both subgroups have retained basic CRT functions, including ER Ca2+-holding potential and putative chaperone capabilities. However, other more general cellular defects due to the absence of CRT in the fibroblasts, such as cell adhesion deficiencies, were not fully restored. Furthermore, in planta expression, protein localization and mutant analyses revealed that the three Arabidopsis CRTs have acquired specialized functions. The AtCRT1a and CRT1b family members appear to be components of a general ER chaperone network. In contrast, and as recently shown, AtCRT3 is associated with immune responses, and is essential for responsiveness to the bacterial Pathogen-Associated Molecular Pattern (PAMP) elf18, derived from elongation factor (EF)-Tu. Whereas constitutively expressed AtCRT1a fully complemented Atcrt1b mutants, AtCRT3 did not.We conclude that the physiological functions of the two CRT subgroups in Arabidopsis have diverged, resulting in a role for AtCRT3 in PAMP associated responses, and possibly more general chaperone functions for AtCRT1a and CRT1b.http://europepmc.org/articles/PMC2893204?pdf=render |
| spellingShingle | Anna Christensen Karin Svensson Lisa Thelin Wenjing Zhang Nico Tintor Daniel Prins Norma Funke Marek Michalak Paul Schulze-Lefert Yusuke Saijo Marianne Sommarin Susanne Widell Staffan Persson Higher plant calreticulins have acquired specialized functions in Arabidopsis. PLoS ONE |
| title | Higher plant calreticulins have acquired specialized functions in Arabidopsis. |
| title_full | Higher plant calreticulins have acquired specialized functions in Arabidopsis. |
| title_fullStr | Higher plant calreticulins have acquired specialized functions in Arabidopsis. |
| title_full_unstemmed | Higher plant calreticulins have acquired specialized functions in Arabidopsis. |
| title_short | Higher plant calreticulins have acquired specialized functions in Arabidopsis. |
| title_sort | higher plant calreticulins have acquired specialized functions in arabidopsis |
| url | http://europepmc.org/articles/PMC2893204?pdf=render |
| work_keys_str_mv | AT annachristensen higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT karinsvensson higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT lisathelin higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT wenjingzhang higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT nicotintor higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT danielprins higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT normafunke higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT marekmichalak higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT paulschulzelefert higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT yusukesaijo higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT mariannesommarin higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT susannewidell higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis AT staffanpersson higherplantcalreticulinshaveacquiredspecializedfunctionsinarabidopsis |