Participation of NO-synthase in Control of Nitric Oxide Level in Rat Hippocampus after Modelling of Ischaemic and Haemorrhagic Insult
Electron paramagnetic resonance (EPR) was used as a method for recording the content of the nitric oxide (NO) in hippocampal tissues of intact rats and rats after modelling of ischaemic and haemorrhagic stroke. Based on direct measurements of NO by EPR spectroscopy, it was shown that, within 5 hours...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2017-10-01
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| Series: | BioDiscovery |
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| Online Access: | https://biodiscovery.pensoft.net/article/14810/download/pdf/ |
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| author | Khalil L. Gainutdinov Svetlana G. Pashkevich Vyatcheslav V. Andrianov Guzel G. Yafarova Margarita O. Dosina Tatiana Kh. Bogodvid Julia P. Stukach Dinara I. Silant'eva Aleksandra S. Zamaro Timur V. Sushko Vladimir Kulchitsky |
| author_facet | Khalil L. Gainutdinov Svetlana G. Pashkevich Vyatcheslav V. Andrianov Guzel G. Yafarova Margarita O. Dosina Tatiana Kh. Bogodvid Julia P. Stukach Dinara I. Silant'eva Aleksandra S. Zamaro Timur V. Sushko Vladimir Kulchitsky |
| author_sort | Khalil L. Gainutdinov |
| collection | DOAJ |
| description | Electron paramagnetic resonance (EPR) was used as a method for recording the content of the nitric oxide (NO) in hippocampal tissues of intact rats and rats after modelling of ischaemic and haemorrhagic stroke. Based on direct measurements of NO by EPR spectroscopy, it was shown that, within 5 hours after the onset of symptoms of ischaemic and haemorrhagic stroke, the formation of NO in the hippocampus was reduced by a factor of 2-3 and this reduction was maintained for a period of between 24 and 72 hours. The results show that a systemic character of a decrease in the intensity of NO production during the modelling of ischaemic events in the brain reflects the effects of central dysregulation of the functions at the level of the whole organism such that it is appropriate to consider implementing the correction of the vital systems of the body in a stroke. It has indicated that non-selective NO-synthase blocker L-NAME reduced the low level of NO production by a factor of 3 by its administration within 72 hours after post-ischaemic and haemorrhagic stroke. It was discovered however that L-NAME returns the level of NO production to baseline (control) by its administration within 5 hours after ischaemia. |
| first_indexed | 2024-12-14T05:03:23Z |
| format | Article |
| id | doaj.art-8968f35e7f214bb5a972952c9584bc2c |
| institution | Directory Open Access Journal |
| issn | 2050-2966 |
| language | English |
| last_indexed | 2024-12-14T05:03:23Z |
| publishDate | 2017-10-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | BioDiscovery |
| spelling | doaj.art-8968f35e7f214bb5a972952c9584bc2c2022-12-21T23:16:11ZengPensoft PublishersBioDiscovery2050-29662017-10-012011310.3897/biodiscovery.20.e1481014810Participation of NO-synthase in Control of Nitric Oxide Level in Rat Hippocampus after Modelling of Ischaemic and Haemorrhagic InsultKhalil L. Gainutdinov0Svetlana G. Pashkevich1Vyatcheslav V. Andrianov2Guzel G. Yafarova3Margarita O. Dosina4Tatiana Kh. Bogodvid5Julia P. Stukach6Dinara I. Silant'eva7Aleksandra S. Zamaro 8Timur V. Sushko9Vladimir Kulchitsky10Russian Academy of SciencesNational Academy of Sciences of BelarusRussian Academy of SciencesRussian Academy of SciencesNational Academy of Sciences of BelarusKazan Federal UniversityNational Academy of Sciences of BelarusKazan Federal UniversityNational Academy of Sciences of BelarusNational Academy of Sciences of BelarusNational Academy of Sciences of BelarusElectron paramagnetic resonance (EPR) was used as a method for recording the content of the nitric oxide (NO) in hippocampal tissues of intact rats and rats after modelling of ischaemic and haemorrhagic stroke. Based on direct measurements of NO by EPR spectroscopy, it was shown that, within 5 hours after the onset of symptoms of ischaemic and haemorrhagic stroke, the formation of NO in the hippocampus was reduced by a factor of 2-3 and this reduction was maintained for a period of between 24 and 72 hours. The results show that a systemic character of a decrease in the intensity of NO production during the modelling of ischaemic events in the brain reflects the effects of central dysregulation of the functions at the level of the whole organism such that it is appropriate to consider implementing the correction of the vital systems of the body in a stroke. It has indicated that non-selective NO-synthase blocker L-NAME reduced the low level of NO production by a factor of 3 by its administration within 72 hours after post-ischaemic and haemorrhagic stroke. It was discovered however that L-NAME returns the level of NO production to baseline (control) by its administration within 5 hours after ischaemia.https://biodiscovery.pensoft.net/article/14810/download/pdf/Nitric oxideElectron paramagnetic resonance |
| spellingShingle | Khalil L. Gainutdinov Svetlana G. Pashkevich Vyatcheslav V. Andrianov Guzel G. Yafarova Margarita O. Dosina Tatiana Kh. Bogodvid Julia P. Stukach Dinara I. Silant'eva Aleksandra S. Zamaro Timur V. Sushko Vladimir Kulchitsky Participation of NO-synthase in Control of Nitric Oxide Level in Rat Hippocampus after Modelling of Ischaemic and Haemorrhagic Insult BioDiscovery Nitric oxide Electron paramagnetic resonance |
| title | Participation of NO-synthase in Control of Nitric Oxide Level in Rat Hippocampus after Modelling of Ischaemic and Haemorrhagic Insult |
| title_full | Participation of NO-synthase in Control of Nitric Oxide Level in Rat Hippocampus after Modelling of Ischaemic and Haemorrhagic Insult |
| title_fullStr | Participation of NO-synthase in Control of Nitric Oxide Level in Rat Hippocampus after Modelling of Ischaemic and Haemorrhagic Insult |
| title_full_unstemmed | Participation of NO-synthase in Control of Nitric Oxide Level in Rat Hippocampus after Modelling of Ischaemic and Haemorrhagic Insult |
| title_short | Participation of NO-synthase in Control of Nitric Oxide Level in Rat Hippocampus after Modelling of Ischaemic and Haemorrhagic Insult |
| title_sort | participation of no synthase in control of nitric oxide level in rat hippocampus after modelling of ischaemic and haemorrhagic insult |
| topic | Nitric oxide Electron paramagnetic resonance |
| url | https://biodiscovery.pensoft.net/article/14810/download/pdf/ |
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