Platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia
Abstract Background Thrombocytopenia is a feared complication of preeclampsia (PE) that can additionally complicate the disease course and that carries a poor prognosis. The disease mechanisms of PE on a platelet level are poorly understood and only few platelet-based markers have been investigated....
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | Molecular Medicine |
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| Online Access: | https://doi.org/10.1186/s10020-022-00472-x |
| _version_ | 1828344468859781120 |
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| author | Bjoern F. Kraemer Irina Hennis Anne Karge Anne Katrin Kraemer Tobias F. Dreyer Marion Kiechle Bettina Kuschel Holger Bronger |
| author_facet | Bjoern F. Kraemer Irina Hennis Anne Karge Anne Katrin Kraemer Tobias F. Dreyer Marion Kiechle Bettina Kuschel Holger Bronger |
| author_sort | Bjoern F. Kraemer |
| collection | DOAJ |
| description | Abstract Background Thrombocytopenia is a feared complication of preeclampsia (PE) that can additionally complicate the disease course and that carries a poor prognosis. The disease mechanisms of PE on a platelet level are poorly understood and only few platelet-based markers have been investigated. In sepsis, platelet mitochondrial membrane depolarization, a sensitive and early indicator of mitochondrial dysfunction and platelet cell death, correlates with disease severity and outcome as shown in previous studies. The aim of this study was to investigate platelet mitochondrial membrane potential (Mmp-Index) by flow-cytometry in patients with preeclampsia compared to controls and to assess its value in correlation with disease severity of PE and during follow-up after delivery. Methods In this prospective translational case–control study, platelet Mmp-Index was measured in PE (n = 16) by flow cytometry in living platelets in simultaneous comparison to healthy pregnant (n = 32) and non-pregnant controls (n = 16) and was individually reassessed after delivery to investigate recovery of platelet mitochondrial function. Subgroup analysis of patients with severe and non-severe PE was performed. Six patients with isolated gestational hypertension were also included for comparative analysis. Results Platelet Mmp-Index in patients with symptomatic preeclampsia (Mmp-Index non-severe PE 0.72 ([0.591; 0.861]; p = 0.002) was significantly reduced compared to healthy pregnant controls (Mmp-Index 0.97 [0.795; 1.117]) and even more pronounced in patients with severe PE (n = 6) (Mmp-Index severe PE 0.542 [0.361; 0.623]; p = 0.03). In the severe PE group, complementary measurements of platelet Annexin V- and CD62 (P-Selectin) surface expression showed apoptosis of platelet populations in the majority of patients. Platelet Mmp normalized after delivery within few days. Patients with isolated gestational hypertension showed normal Mmp-Index values. Conclusions This study shows for the first time that platelet Mmp-Index is a quantifiable, easy-to-measure intracellular marker of platelet mitochondrial function in vital cells that reflects disease severity of preeclampsia. For future investigations, platelet Mmp may serve as a prognostic marker that may aid clinical risk stratification and adds novel information on potential mechanisms for thrombocytopenia in preeclampsia. |
| first_indexed | 2024-04-13T23:57:36Z |
| format | Article |
| id | doaj.art-896cfbf6d15d4ab9aa50ccb880912453 |
| institution | Directory Open Access Journal |
| issn | 1076-1551 1528-3658 |
| language | English |
| last_indexed | 2024-04-13T23:57:36Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Medicine |
| spelling | doaj.art-896cfbf6d15d4ab9aa50ccb8809124532022-12-22T02:23:49ZengBMCMolecular Medicine1076-15511528-36582022-05-012811910.1186/s10020-022-00472-xPlatelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsiaBjoern F. Kraemer0Irina Hennis1Anne Karge2Anne Katrin Kraemer3Tobias F. Dreyer4Marion Kiechle5Bettina Kuschel6Holger Bronger7Medizinische Klinik Und Poliklinik I, LMU KlinikumDepartment of Gynecology and Obstetrics, Technical University of MunichDepartment of Gynecology and Obstetrics, Technical University of MunichDepartment of Gynecology and Obstetrics, Technical University of MunichDepartment of Gynecology and Obstetrics, Technical University of MunichDepartment of Gynecology and Obstetrics, Technical University of MunichDepartment of Gynecology and Obstetrics, Technical University of MunichDepartment of Gynecology and Obstetrics, Technical University of MunichAbstract Background Thrombocytopenia is a feared complication of preeclampsia (PE) that can additionally complicate the disease course and that carries a poor prognosis. The disease mechanisms of PE on a platelet level are poorly understood and only few platelet-based markers have been investigated. In sepsis, platelet mitochondrial membrane depolarization, a sensitive and early indicator of mitochondrial dysfunction and platelet cell death, correlates with disease severity and outcome as shown in previous studies. The aim of this study was to investigate platelet mitochondrial membrane potential (Mmp-Index) by flow-cytometry in patients with preeclampsia compared to controls and to assess its value in correlation with disease severity of PE and during follow-up after delivery. Methods In this prospective translational case–control study, platelet Mmp-Index was measured in PE (n = 16) by flow cytometry in living platelets in simultaneous comparison to healthy pregnant (n = 32) and non-pregnant controls (n = 16) and was individually reassessed after delivery to investigate recovery of platelet mitochondrial function. Subgroup analysis of patients with severe and non-severe PE was performed. Six patients with isolated gestational hypertension were also included for comparative analysis. Results Platelet Mmp-Index in patients with symptomatic preeclampsia (Mmp-Index non-severe PE 0.72 ([0.591; 0.861]; p = 0.002) was significantly reduced compared to healthy pregnant controls (Mmp-Index 0.97 [0.795; 1.117]) and even more pronounced in patients with severe PE (n = 6) (Mmp-Index severe PE 0.542 [0.361; 0.623]; p = 0.03). In the severe PE group, complementary measurements of platelet Annexin V- and CD62 (P-Selectin) surface expression showed apoptosis of platelet populations in the majority of patients. Platelet Mmp normalized after delivery within few days. Patients with isolated gestational hypertension showed normal Mmp-Index values. Conclusions This study shows for the first time that platelet Mmp-Index is a quantifiable, easy-to-measure intracellular marker of platelet mitochondrial function in vital cells that reflects disease severity of preeclampsia. For future investigations, platelet Mmp may serve as a prognostic marker that may aid clinical risk stratification and adds novel information on potential mechanisms for thrombocytopenia in preeclampsia.https://doi.org/10.1186/s10020-022-00472-xPreeclampsiaPlateletsMitochondrial membrane potential |
| spellingShingle | Bjoern F. Kraemer Irina Hennis Anne Karge Anne Katrin Kraemer Tobias F. Dreyer Marion Kiechle Bettina Kuschel Holger Bronger Platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia Molecular Medicine Preeclampsia Platelets Mitochondrial membrane potential |
| title | Platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia |
| title_full | Platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia |
| title_fullStr | Platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia |
| title_full_unstemmed | Platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia |
| title_short | Platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia |
| title_sort | platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia |
| topic | Preeclampsia Platelets Mitochondrial membrane potential |
| url | https://doi.org/10.1186/s10020-022-00472-x |
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