MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10
Background/Aims: Human bone marrow-derived mesenchymal stem cells (hMSCs) are a promising cell source for bone engineering owing to their high potential to differentiate into osteoblasts. The bone morphogenetic protein-inducible gene homeobox a10 (HOXA10) is a critical regulator of osteogenesis. The...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2016-01-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/438607 |
| _version_ | 1819158420390936576 |
|---|---|
| author | Jianhou Huang Yake Meng Yan Liu Yu Chen Haisong Yang Deyu Chen Jiangang Shi Yongfei Guo |
| author_facet | Jianhou Huang Yake Meng Yan Liu Yu Chen Haisong Yang Deyu Chen Jiangang Shi Yongfei Guo |
| author_sort | Jianhou Huang |
| collection | DOAJ |
| description | Background/Aims: Human bone marrow-derived mesenchymal stem cells (hMSCs) are a promising cell source for bone engineering owing to their high potential to differentiate into osteoblasts. The bone morphogenetic protein-inducible gene homeobox a10 (HOXA10) is a critical regulator of osteogenesis. The objective of the present study was to identify microR-NAs (miRNAs) targeting HOXA10 and examine the effects on the osteogenic differentiation of hMSCs. Methods: Based on in silico analysis, HOXA10-targeting miRNAs were selected and their regulatory roles in osteoblast differentiation were investigated. Results: Six HOXA10-targeting miRNAs were identifIed by computational analysis, of which miR-320a was selected for further analysis because it was downregulated during osteogenic induction. Overexpression of miR-320a downregulated HOXA10 and significantly inhibited osteogenesis in hMSCs, as determined by the downregulation of the osteogenic markers Runx2, ALP, and OC and the inhibition of ALP activity and matrix mineralization, whereas miR-320a inhibition had the opposite effects. Furthermore, ectopic expression of HOXA10 (not including 3′-UTR) rescued the effects of miR-320a on osteogenic differentiation. Conclusion: These results suggest that miR-320a acts as a critical regulator of osteogenic differentiation of hMSCs by repressing its target HOXA10. |
| first_indexed | 2024-12-22T16:24:22Z |
| format | Article |
| id | doaj.art-896ebaa0af674ccc89a90b3a5a9cbd6d |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-22T16:24:22Z |
| publishDate | 2016-01-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-896ebaa0af674ccc89a90b3a5a9cbd6d2022-12-21T18:20:12ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-01-01381404810.1159/000438607438607MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10Jianhou HuangYake MengYan LiuYu ChenHaisong YangDeyu ChenJiangang ShiYongfei GuoBackground/Aims: Human bone marrow-derived mesenchymal stem cells (hMSCs) are a promising cell source for bone engineering owing to their high potential to differentiate into osteoblasts. The bone morphogenetic protein-inducible gene homeobox a10 (HOXA10) is a critical regulator of osteogenesis. The objective of the present study was to identify microR-NAs (miRNAs) targeting HOXA10 and examine the effects on the osteogenic differentiation of hMSCs. Methods: Based on in silico analysis, HOXA10-targeting miRNAs were selected and their regulatory roles in osteoblast differentiation were investigated. Results: Six HOXA10-targeting miRNAs were identifIed by computational analysis, of which miR-320a was selected for further analysis because it was downregulated during osteogenic induction. Overexpression of miR-320a downregulated HOXA10 and significantly inhibited osteogenesis in hMSCs, as determined by the downregulation of the osteogenic markers Runx2, ALP, and OC and the inhibition of ALP activity and matrix mineralization, whereas miR-320a inhibition had the opposite effects. Furthermore, ectopic expression of HOXA10 (not including 3′-UTR) rescued the effects of miR-320a on osteogenic differentiation. Conclusion: These results suggest that miR-320a acts as a critical regulator of osteogenic differentiation of hMSCs by repressing its target HOXA10.http://www.karger.com/Article/FullText/438607MiR-320aHOXA10Bone marrow-derived mesenchymal stem cellsOsteogenic differentiation |
| spellingShingle | Jianhou Huang Yake Meng Yan Liu Yu Chen Haisong Yang Deyu Chen Jiangang Shi Yongfei Guo MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10 Cellular Physiology and Biochemistry MiR-320a HOXA10 Bone marrow-derived mesenchymal stem cells Osteogenic differentiation |
| title | MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10 |
| title_full | MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10 |
| title_fullStr | MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10 |
| title_full_unstemmed | MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10 |
| title_short | MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10 |
| title_sort | microrna 320a regulates the osteogenic differentiation of human bone marrow derived mesenchymal stem cells by targeting hoxa10 |
| topic | MiR-320a HOXA10 Bone marrow-derived mesenchymal stem cells Osteogenic differentiation |
| url | http://www.karger.com/Article/FullText/438607 |
| work_keys_str_mv | AT jianhouhuang microrna320aregulatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsbytargetinghoxa10 AT yakemeng microrna320aregulatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsbytargetinghoxa10 AT yanliu microrna320aregulatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsbytargetinghoxa10 AT yuchen microrna320aregulatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsbytargetinghoxa10 AT haisongyang microrna320aregulatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsbytargetinghoxa10 AT deyuchen microrna320aregulatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsbytargetinghoxa10 AT jiangangshi microrna320aregulatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsbytargetinghoxa10 AT yongfeiguo microrna320aregulatestheosteogenicdifferentiationofhumanbonemarrowderivedmesenchymalstemcellsbytargetinghoxa10 |