Expression pattern of genes involved in biomineralization in black and orange mantle tissues of pearl oyster, Pinctada persica
A few species of mollusks display color variation in their soft tissues. In pearl oysters, the color polymorphism in mantle tissue is associated with the color and radiance of shell and pearl. The study of biomineralization related genes in mantle tissue of pearl oysters can be used as a suitable ap...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.1038692/full |
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| author | Fatemeh Parvizi Arash Akbarzadeh Ahmad Farhadi Sophie Arnaud-Haond Mohammad Sharif Ranjbar |
| author_facet | Fatemeh Parvizi Arash Akbarzadeh Ahmad Farhadi Sophie Arnaud-Haond Mohammad Sharif Ranjbar |
| author_sort | Fatemeh Parvizi |
| collection | DOAJ |
| description | A few species of mollusks display color variation in their soft tissues. In pearl oysters, the color polymorphism in mantle tissue is associated with the color and radiance of shell and pearl. The study of biomineralization related genes in mantle tissue of pearl oysters can be used as a suitable approach to better identify the molecular mechanisms that influence shell and pearl quality and color variations. In this study, we investigated the expression of biomineralization-related genes in black and orange mantle morphotypes of pearl oyster, Pinctada persica in both warm and cool seasons using quantitative real-time PCR. Our results showed that the genes involved in biomineralization of the prismatic and nacre layer, i.e.; ASP, KRMP, MRNP34, SHELL, SHEM1B, LINKINE, PIF, SHEM5, NACREIN, and in pigmentation (TYR2A) were significantly higher expressed in orange phenotype compared to those of black one. The higher expression of ASP, KRMP, SHEM5, LINKINE and NACREIN in orange phenotype was only observed in warm season, but PIF, SHELL, SHEM1B, and TYR2A were upregulated in both warm and cool seasons. These results suggest the existence of different genetic processes between the two color morphs of P. persica and the more active role of genes in orange morphotype, particularly in warmer season. This study provides better understanding of the molecular mechanisms underlying biomineralization in pearl oysters. |
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| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-10T21:38:14Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Marine Science |
| spelling | doaj.art-8971d6f144be4e50bda3abaf70612bb22023-01-19T08:56:43ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-01-01910.3389/fmars.2022.10386921038692Expression pattern of genes involved in biomineralization in black and orange mantle tissues of pearl oyster, Pinctada persicaFatemeh Parvizi0Arash Akbarzadeh1Ahmad Farhadi2Sophie Arnaud-Haond3Mohammad Sharif Ranjbar4Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, IranDepartment of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, IranDepartment of Natural Resources and Environmental Engineering, School of Agriculture, Shiraz University, Shiraz, IranMarine Biodiversity Exploitation and Conservation (MARBEC), UMR 9190, Univ. Montpellier, IFREMER, IRD, CNRS, Sète, FranceDepartment of Marine Biology, Faculty of Marine Science and technology, University of Hormozgan, Bandar Abbas, IranA few species of mollusks display color variation in their soft tissues. In pearl oysters, the color polymorphism in mantle tissue is associated with the color and radiance of shell and pearl. The study of biomineralization related genes in mantle tissue of pearl oysters can be used as a suitable approach to better identify the molecular mechanisms that influence shell and pearl quality and color variations. In this study, we investigated the expression of biomineralization-related genes in black and orange mantle morphotypes of pearl oyster, Pinctada persica in both warm and cool seasons using quantitative real-time PCR. Our results showed that the genes involved in biomineralization of the prismatic and nacre layer, i.e.; ASP, KRMP, MRNP34, SHELL, SHEM1B, LINKINE, PIF, SHEM5, NACREIN, and in pigmentation (TYR2A) were significantly higher expressed in orange phenotype compared to those of black one. The higher expression of ASP, KRMP, SHEM5, LINKINE and NACREIN in orange phenotype was only observed in warm season, but PIF, SHELL, SHEM1B, and TYR2A were upregulated in both warm and cool seasons. These results suggest the existence of different genetic processes between the two color morphs of P. persica and the more active role of genes in orange morphotype, particularly in warmer season. This study provides better understanding of the molecular mechanisms underlying biomineralization in pearl oysters.https://www.frontiersin.org/articles/10.3389/fmars.2022.1038692/fullgene expressionmantle colorbiomineralizationqRT-PCRpearl oyster |
| spellingShingle | Fatemeh Parvizi Arash Akbarzadeh Ahmad Farhadi Sophie Arnaud-Haond Mohammad Sharif Ranjbar Expression pattern of genes involved in biomineralization in black and orange mantle tissues of pearl oyster, Pinctada persica Frontiers in Marine Science gene expression mantle color biomineralization qRT-PCR pearl oyster |
| title | Expression pattern of genes involved in biomineralization in black and orange mantle tissues of pearl oyster, Pinctada persica |
| title_full | Expression pattern of genes involved in biomineralization in black and orange mantle tissues of pearl oyster, Pinctada persica |
| title_fullStr | Expression pattern of genes involved in biomineralization in black and orange mantle tissues of pearl oyster, Pinctada persica |
| title_full_unstemmed | Expression pattern of genes involved in biomineralization in black and orange mantle tissues of pearl oyster, Pinctada persica |
| title_short | Expression pattern of genes involved in biomineralization in black and orange mantle tissues of pearl oyster, Pinctada persica |
| title_sort | expression pattern of genes involved in biomineralization in black and orange mantle tissues of pearl oyster pinctada persica |
| topic | gene expression mantle color biomineralization qRT-PCR pearl oyster |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.1038692/full |
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