No evidence of geographical structure of salicinoid chemotypes within Populus tremula.
Salicinoids are well-known defense compounds in salicaceous trees and careful screening at the population level is warranted to fully understand their diversity and function. European aspen, Populus tremula, is a foundation species in Eurasia and highly polymorphic in Sweden. We exhaustively surveye...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4191948?pdf=render |
| _version_ | 1818132916506460160 |
|---|---|
| author | Ken Keefover-Ring Maria Ahnlund Ilka Nacif Abreu Stefan Jansson Thomas Moritz Benedicte Riber Albrectsen |
| author_facet | Ken Keefover-Ring Maria Ahnlund Ilka Nacif Abreu Stefan Jansson Thomas Moritz Benedicte Riber Albrectsen |
| author_sort | Ken Keefover-Ring |
| collection | DOAJ |
| description | Salicinoids are well-known defense compounds in salicaceous trees and careful screening at the population level is warranted to fully understand their diversity and function. European aspen, Populus tremula, is a foundation species in Eurasia and highly polymorphic in Sweden. We exhaustively surveyed 102 replicated genotypes from the Swedish Aspen collection (SwAsp) for foliar salicinoids using UHPLC-ESI-TOF/MS and identified nine novel compounds, bringing the total to 19 for this species. Salicinoid structure followed a modular architecture of a salicin skeleton with added side groups, alone or in combination. Two main moieties, 2'-cinnamoyl and 2'-acetyl, grouped the SwAsp population into four distinct chemotypes, and the relative allocation of salicinoids was remarkably constant between different environments, implying a highly channeled biosynthesis of these compounds. Slightly more than half of the SwAsp genotypes belonged to the cinnamoyl chemotype. A fraction synthesized the acetyl moiety alone (∼7%) or in combination with cinnamoyl (∼2%), and close to forty percent lacked either of the two characteristic moieties, and thus resemble P. tremuloides in their salicinoid profile. The two most abundant chemotypes were evenly distributed throughout Sweden, unlike geographical patterns reported for SwAsp phenology traits, plant defense genes, and herbivore community associations. Here we present the salicinoid characterization of the SwAsp collection as a resource for future studies of aspen chemical ecology, salicinoid biosynthesis, and genetics. |
| first_indexed | 2024-12-11T08:44:26Z |
| format | Article |
| id | doaj.art-897294b8d25e44af98acdc48eb0535d1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:44:26Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-897294b8d25e44af98acdc48eb0535d12022-12-22T01:14:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e10718910.1371/journal.pone.0107189No evidence of geographical structure of salicinoid chemotypes within Populus tremula.Ken Keefover-RingMaria AhnlundIlka Nacif AbreuStefan JanssonThomas MoritzBenedicte Riber AlbrectsenSalicinoids are well-known defense compounds in salicaceous trees and careful screening at the population level is warranted to fully understand their diversity and function. European aspen, Populus tremula, is a foundation species in Eurasia and highly polymorphic in Sweden. We exhaustively surveyed 102 replicated genotypes from the Swedish Aspen collection (SwAsp) for foliar salicinoids using UHPLC-ESI-TOF/MS and identified nine novel compounds, bringing the total to 19 for this species. Salicinoid structure followed a modular architecture of a salicin skeleton with added side groups, alone or in combination. Two main moieties, 2'-cinnamoyl and 2'-acetyl, grouped the SwAsp population into four distinct chemotypes, and the relative allocation of salicinoids was remarkably constant between different environments, implying a highly channeled biosynthesis of these compounds. Slightly more than half of the SwAsp genotypes belonged to the cinnamoyl chemotype. A fraction synthesized the acetyl moiety alone (∼7%) or in combination with cinnamoyl (∼2%), and close to forty percent lacked either of the two characteristic moieties, and thus resemble P. tremuloides in their salicinoid profile. The two most abundant chemotypes were evenly distributed throughout Sweden, unlike geographical patterns reported for SwAsp phenology traits, plant defense genes, and herbivore community associations. Here we present the salicinoid characterization of the SwAsp collection as a resource for future studies of aspen chemical ecology, salicinoid biosynthesis, and genetics.http://europepmc.org/articles/PMC4191948?pdf=render |
| spellingShingle | Ken Keefover-Ring Maria Ahnlund Ilka Nacif Abreu Stefan Jansson Thomas Moritz Benedicte Riber Albrectsen No evidence of geographical structure of salicinoid chemotypes within Populus tremula. PLoS ONE |
| title | No evidence of geographical structure of salicinoid chemotypes within Populus tremula. |
| title_full | No evidence of geographical structure of salicinoid chemotypes within Populus tremula. |
| title_fullStr | No evidence of geographical structure of salicinoid chemotypes within Populus tremula. |
| title_full_unstemmed | No evidence of geographical structure of salicinoid chemotypes within Populus tremula. |
| title_short | No evidence of geographical structure of salicinoid chemotypes within Populus tremula. |
| title_sort | no evidence of geographical structure of salicinoid chemotypes within populus tremula |
| url | http://europepmc.org/articles/PMC4191948?pdf=render |
| work_keys_str_mv | AT kenkeefoverring noevidenceofgeographicalstructureofsalicinoidchemotypeswithinpopulustremula AT mariaahnlund noevidenceofgeographicalstructureofsalicinoidchemotypeswithinpopulustremula AT ilkanacifabreu noevidenceofgeographicalstructureofsalicinoidchemotypeswithinpopulustremula AT stefanjansson noevidenceofgeographicalstructureofsalicinoidchemotypeswithinpopulustremula AT thomasmoritz noevidenceofgeographicalstructureofsalicinoidchemotypeswithinpopulustremula AT benedicteriberalbrectsen noevidenceofgeographicalstructureofsalicinoidchemotypeswithinpopulustremula |