In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus
Purpose: Reovirus type 3 Dearing (ReoT3D), a wild type oncolytic virus (OV) from the Reoviridae family, kills KRAS mutant cancer cells. However, the use of OVs has faced with some limitations such as immune responses, and delivery of OVs to the tumor sites in systemic therapy. To solve this, and als...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2021-02-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | https://apb.tbzmed.ac.ir/PDF/apb-11-361.pdf |
| _version_ | 1818890705963057152 |
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| author | Abouzar Babaei Hossein Bannazadeh Baghi Akram Nezhadi Zahra Jamalpoor |
| author_facet | Abouzar Babaei Hossein Bannazadeh Baghi Akram Nezhadi Zahra Jamalpoor |
| author_sort | Abouzar Babaei |
| collection | DOAJ |
| description | Purpose: Reovirus type 3 Dearing (ReoT3D), a wild type oncolytic virus (OV) from the Reoviridae family, kills KRAS mutant cancer cells. However, the use of OVs has faced with some limitations such as immune responses, and delivery of OVs to the tumor sites in systemic therapy. To solve this, and also to increase the anti-cancer effects of these OVs, mesenchymal stem cells (MSCs) might be used as an effective vehicle for OVs delivery. In this study, we examined the anti-cancer effects of human adipose derived-MSCs (AD-MSCs) as a vehicle of ReoT3D against human glioblastoma cells. Methods: Here, AD-MSCs were characterized and toxicity of ReoT3D on them was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Then, capability of AD-MSCs for virus production was assessed by real-time polymerase chain reaction (PCR), and different in vitro anti-cancer experiments were applied for our anti-cancer purposes. Results: Our results from toxicity assay revealed that the isolated and provoked AD-MSCs were resistant to nontoxic concentration multiplicity of infection (MOI) >1 pfu/cells of ReoT3D. In addition, the results indicated that AD-MSCs were susceptible for virus life cycle complementation and were capable for production of virus progenies. Furthermore, our results showed that AD-MSCs had oncolysis effects and increased the anti-cancer effects of ReoT3D. Conclusion: AD-MSCs as a susceptible host for oncolytic reovirus could increase the anti-cancer activity of this OV against glioblastoma multiforme (GBM) cell line. |
| first_indexed | 2024-12-19T17:29:10Z |
| format | Article |
| id | doaj.art-8973880282d346bb937cbd312101e82a |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-19T17:29:10Z |
| publishDate | 2021-02-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-8973880282d346bb937cbd312101e82a2022-12-21T20:12:29ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082021-02-0111236137010.34172/apb.2021.034apb-28670In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic ReovirusAbouzar Babaei0Hossein Bannazadeh Baghi1Akram Nezhadi2Zahra Jamalpoor3Trauma Research Center, Aja University of Medical Sciences, Tehran, Iran.Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Neuroscience Research Center, Aja University of Medical Sciences, Tehran, Iran.Trauma Research Center, Aja University of Medical Sciences, Tehran, Iran.Purpose: Reovirus type 3 Dearing (ReoT3D), a wild type oncolytic virus (OV) from the Reoviridae family, kills KRAS mutant cancer cells. However, the use of OVs has faced with some limitations such as immune responses, and delivery of OVs to the tumor sites in systemic therapy. To solve this, and also to increase the anti-cancer effects of these OVs, mesenchymal stem cells (MSCs) might be used as an effective vehicle for OVs delivery. In this study, we examined the anti-cancer effects of human adipose derived-MSCs (AD-MSCs) as a vehicle of ReoT3D against human glioblastoma cells. Methods: Here, AD-MSCs were characterized and toxicity of ReoT3D on them was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Then, capability of AD-MSCs for virus production was assessed by real-time polymerase chain reaction (PCR), and different in vitro anti-cancer experiments were applied for our anti-cancer purposes. Results: Our results from toxicity assay revealed that the isolated and provoked AD-MSCs were resistant to nontoxic concentration multiplicity of infection (MOI) >1 pfu/cells of ReoT3D. In addition, the results indicated that AD-MSCs were susceptible for virus life cycle complementation and were capable for production of virus progenies. Furthermore, our results showed that AD-MSCs had oncolysis effects and increased the anti-cancer effects of ReoT3D. Conclusion: AD-MSCs as a susceptible host for oncolytic reovirus could increase the anti-cancer activity of this OV against glioblastoma multiforme (GBM) cell line.https://apb.tbzmed.ac.ir/PDF/apb-11-361.pdfoncolytic virusreovirus type 3 dearingmesenchymal stem cellglioblastoma cancer |
| spellingShingle | Abouzar Babaei Hossein Bannazadeh Baghi Akram Nezhadi Zahra Jamalpoor In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus Advanced Pharmaceutical Bulletin oncolytic virus reovirus type 3 dearing mesenchymal stem cell glioblastoma cancer |
| title | In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus |
| title_full | In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus |
| title_fullStr | In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus |
| title_full_unstemmed | In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus |
| title_short | In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus |
| title_sort | in vitro anti cancer activity of adipose derived mesenchymal stem cells increased after infection with oncolytic reovirus |
| topic | oncolytic virus reovirus type 3 dearing mesenchymal stem cell glioblastoma cancer |
| url | https://apb.tbzmed.ac.ir/PDF/apb-11-361.pdf |
| work_keys_str_mv | AT abouzarbabaei invitroanticanceractivityofadiposederivedmesenchymalstemcellsincreasedafterinfectionwithoncolyticreovirus AT hosseinbannazadehbaghi invitroanticanceractivityofadiposederivedmesenchymalstemcellsincreasedafterinfectionwithoncolyticreovirus AT akramnezhadi invitroanticanceractivityofadiposederivedmesenchymalstemcellsincreasedafterinfectionwithoncolyticreovirus AT zahrajamalpoor invitroanticanceractivityofadiposederivedmesenchymalstemcellsincreasedafterinfectionwithoncolyticreovirus |