Bactericidal activities and biochemical analysis of skin mucus of Cyprinid fish
This study reports the bactericidal activity of mucus extracts and biochemical characterization of skin mucus from five Cyprinids, including Labeo rohita, Ctenopharyngodon Idella, Gibelion catla, Hypophthalmichthys molitrix and Cirrhinus mrigala against ten different bacteria extracted from naturall...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364723001933 |
| _version_ | 1797774823671201792 |
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| author | Shandana Ali Muhammad Nasir Khan Khattak Waheed Ullah Muhammad Rauf Sadir Zaman Farman Ullah Dawar |
| author_facet | Shandana Ali Muhammad Nasir Khan Khattak Waheed Ullah Muhammad Rauf Sadir Zaman Farman Ullah Dawar |
| author_sort | Shandana Ali |
| collection | DOAJ |
| description | This study reports the bactericidal activity of mucus extracts and biochemical characterization of skin mucus from five Cyprinids, including Labeo rohita, Ctenopharyngodon Idella, Gibelion catla, Hypophthalmichthys molitrix and Cirrhinus mrigala against ten different bacteria extracted from naturally infected fish. The bactericidal activity was measured based on the zone of inhibition (ZOI) and compared against Fosfomycin. Importantly, acidic mucus extracts from five fish species exhibited higher bactericidal activity than organic and aqueous extracts. The acidic skin mucus extracts of C. idella, L. rohita, and G. catla showed higher ZOI against Staphylococcus aureus, Aeromonas hydrophila, and Pseudomonas aeruginosa. The minimum inhibitory concentration (MIC) of acidic mucus extracts from C. Idella, L. rohita, and G. catla was 16 µg/mL against A. hydrophila, P. aeruginosa, and S. aureus.Further, biochemical characterization of mucus extracts showed that protein concentration was high in the acidic mucus extracts from L. rohita, C. idella, and G. catla compared to H. molitrix and C. mirigala followed by carbohydrate and lipid content. These findings suggest that skin mucus from cyprinids could be a potent source of innovative bactericidal components for fish and human-related treatments. |
| first_indexed | 2024-03-12T22:26:49Z |
| format | Article |
| id | doaj.art-897422bb5661466ea8cf471432de6871 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-03-12T22:26:49Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-897422bb5661466ea8cf471432de68712023-07-22T04:51:27ZengElsevierJournal of King Saud University: Science1018-36472023-08-01356102731Bactericidal activities and biochemical analysis of skin mucus of Cyprinid fishShandana Ali0Muhammad Nasir Khan Khattak1Waheed Ullah2Muhammad Rauf3Sadir Zaman4Farman Ullah Dawar5Laboratory of Fisheries and Aquaculture, Department of Zoology, Kohat University of Science and Technology Kohat, 26000 Khyber Pakhtunkhwa, PakistanDepartment of Applied Biology, College of Sciences, University of Sharjah, Sharjah 27272, United Arab Emirates; Corresponding authors at: Department of Microbiology, Kohat University of Science and Technology Kohat, 26000 (F.U. Dawar).Department of Microbiology, Kohat University of Science and Technology Kohat, 26000 Khyber Pakhtunkhwa, PakistanLaboratory of Fisheries and Aquaculture, Department of Zoology, Kohat University of Science and Technology Kohat, 26000 Khyber Pakhtunkhwa, PakistanDepartment of Microbiology, Kohat University of Science and Technology Kohat, 26000 Khyber Pakhtunkhwa, PakistanLaboratory of Fisheries and Aquaculture, Department of Zoology, Kohat University of Science and Technology Kohat, 26000 Khyber Pakhtunkhwa, Pakistan; Laboratory of Marine Biology, College of Oceanography, Hohai University, 1 Xikang Road, Nanjing, Jiangsu,210098, China; Corresponding authors at: Department of Microbiology, Kohat University of Science and Technology Kohat, 26000 (F.U. Dawar).This study reports the bactericidal activity of mucus extracts and biochemical characterization of skin mucus from five Cyprinids, including Labeo rohita, Ctenopharyngodon Idella, Gibelion catla, Hypophthalmichthys molitrix and Cirrhinus mrigala against ten different bacteria extracted from naturally infected fish. The bactericidal activity was measured based on the zone of inhibition (ZOI) and compared against Fosfomycin. Importantly, acidic mucus extracts from five fish species exhibited higher bactericidal activity than organic and aqueous extracts. The acidic skin mucus extracts of C. idella, L. rohita, and G. catla showed higher ZOI against Staphylococcus aureus, Aeromonas hydrophila, and Pseudomonas aeruginosa. The minimum inhibitory concentration (MIC) of acidic mucus extracts from C. Idella, L. rohita, and G. catla was 16 µg/mL against A. hydrophila, P. aeruginosa, and S. aureus.Further, biochemical characterization of mucus extracts showed that protein concentration was high in the acidic mucus extracts from L. rohita, C. idella, and G. catla compared to H. molitrix and C. mirigala followed by carbohydrate and lipid content. These findings suggest that skin mucus from cyprinids could be a potent source of innovative bactericidal components for fish and human-related treatments.http://www.sciencedirect.com/science/article/pii/S1018364723001933Antimicrobial componentsBactericidal activityBacterial pathogensBiochemical characterizationCyprinidsSkin mucus |
| spellingShingle | Shandana Ali Muhammad Nasir Khan Khattak Waheed Ullah Muhammad Rauf Sadir Zaman Farman Ullah Dawar Bactericidal activities and biochemical analysis of skin mucus of Cyprinid fish Journal of King Saud University: Science Antimicrobial components Bactericidal activity Bacterial pathogens Biochemical characterization Cyprinids Skin mucus |
| title | Bactericidal activities and biochemical analysis of skin mucus of Cyprinid fish |
| title_full | Bactericidal activities and biochemical analysis of skin mucus of Cyprinid fish |
| title_fullStr | Bactericidal activities and biochemical analysis of skin mucus of Cyprinid fish |
| title_full_unstemmed | Bactericidal activities and biochemical analysis of skin mucus of Cyprinid fish |
| title_short | Bactericidal activities and biochemical analysis of skin mucus of Cyprinid fish |
| title_sort | bactericidal activities and biochemical analysis of skin mucus of cyprinid fish |
| topic | Antimicrobial components Bactericidal activity Bacterial pathogens Biochemical characterization Cyprinids Skin mucus |
| url | http://www.sciencedirect.com/science/article/pii/S1018364723001933 |
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