Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells.
Interactions between B and T cells are essential for most antibody responses, but the dynamics of these interactions are poorly understood. By two-photon microscopy of intact lymph nodes, we show that upon exposure to antigen, B cells migrate with directional preference toward the B-zone-T-zone boun...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2005-06-01
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| Series: | PLoS Biology |
| Online Access: | http://europepmc.org/articles/PMC1088276?pdf=render |
| _version_ | 1819155249592532992 |
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| author | Takaharu Okada Mark J Miller Ian Parker Matthew F Krummel Margaret Neighbors Suzanne B Hartley Anne O'Garra Michael D Cahalan Jason G Cyster |
| author_facet | Takaharu Okada Mark J Miller Ian Parker Matthew F Krummel Margaret Neighbors Suzanne B Hartley Anne O'Garra Michael D Cahalan Jason G Cyster |
| author_sort | Takaharu Okada |
| collection | DOAJ |
| description | Interactions between B and T cells are essential for most antibody responses, but the dynamics of these interactions are poorly understood. By two-photon microscopy of intact lymph nodes, we show that upon exposure to antigen, B cells migrate with directional preference toward the B-zone-T-zone boundary in a CCR7-dependent manner, through a region that exhibits a CCR7-ligand gradient. Initially the B cells show reduced motility, but after 1 d, motility is increased to approximately 9 microm/min. Antigen-engaged B cells pair with antigen-specific helper T cells for 10 to more than 60 min, whereas non-antigen-specific interactions last less than 10 min. B cell-T cell conjugates are highly dynamic and migrate extensively, being led by B cells. B cells occasionally contact more than one T cell, whereas T cells are strictly monogamous in their interactions. These findings provide evidence of lymphocyte chemotaxis in vivo, and they begin to define the spatiotemporal cellular dynamics associated with T cell-dependent antibody responses. |
| first_indexed | 2024-12-22T15:33:59Z |
| format | Article |
| id | doaj.art-8974c5cbbb18411492ced432ef0142e8 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-22T15:33:59Z |
| publishDate | 2005-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-8974c5cbbb18411492ced432ef0142e82022-12-21T18:21:19ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852005-06-0136e15010.1371/journal.pbio.0030150Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells.Takaharu OkadaMark J MillerIan ParkerMatthew F KrummelMargaret NeighborsSuzanne B HartleyAnne O'GarraMichael D CahalanJason G CysterInteractions between B and T cells are essential for most antibody responses, but the dynamics of these interactions are poorly understood. By two-photon microscopy of intact lymph nodes, we show that upon exposure to antigen, B cells migrate with directional preference toward the B-zone-T-zone boundary in a CCR7-dependent manner, through a region that exhibits a CCR7-ligand gradient. Initially the B cells show reduced motility, but after 1 d, motility is increased to approximately 9 microm/min. Antigen-engaged B cells pair with antigen-specific helper T cells for 10 to more than 60 min, whereas non-antigen-specific interactions last less than 10 min. B cell-T cell conjugates are highly dynamic and migrate extensively, being led by B cells. B cells occasionally contact more than one T cell, whereas T cells are strictly monogamous in their interactions. These findings provide evidence of lymphocyte chemotaxis in vivo, and they begin to define the spatiotemporal cellular dynamics associated with T cell-dependent antibody responses.http://europepmc.org/articles/PMC1088276?pdf=render |
| spellingShingle | Takaharu Okada Mark J Miller Ian Parker Matthew F Krummel Margaret Neighbors Suzanne B Hartley Anne O'Garra Michael D Cahalan Jason G Cyster Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells. PLoS Biology |
| title | Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells. |
| title_full | Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells. |
| title_fullStr | Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells. |
| title_full_unstemmed | Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells. |
| title_short | Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells. |
| title_sort | antigen engaged b cells undergo chemotaxis toward the t zone and form motile conjugates with helper t cells |
| url | http://europepmc.org/articles/PMC1088276?pdf=render |
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