Chromosomal Differentiation of Deschampsia (Poaceae) Based on Four Satellite DNA Families
Diverse families of satellite DNA (satDNA) were detected in heterochromatin regions of Deschampsia. This kind of repetitive DNA consists of tandem repeat sequences forming big arrays in genomes, and can contribute to lineages differentiation. The differentiation between types of satDNA is related to...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-09-01
|
| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.728664/full |
| _version_ | 1818358965503787008 |
|---|---|
| author | María Laura González Jorge Oscar Chiapella Juan Domingo Urdampilleta |
| author_facet | María Laura González Jorge Oscar Chiapella Juan Domingo Urdampilleta |
| author_sort | María Laura González |
| collection | DOAJ |
| description | Diverse families of satellite DNA (satDNA) were detected in heterochromatin regions of Deschampsia. This kind of repetitive DNA consists of tandem repeat sequences forming big arrays in genomes, and can contribute to lineages differentiation. The differentiation between types of satDNA is related to their sequence identity, the size and number of monomers forming the array, and their chromosomal location. In this work, four families of satDNA (D2, D3, D12, D13), previously isolated by genomic analysis, were studied on chromosomal preparations of 12 species of Deschampsia (D. airiformis, D. antarctica, D. cespitosa, D. cordillerarum, D. elongata, D. kingii, D. laxa, D. mendocina, D. parvula, D. patula, D. venustula, and Deschampsia sp) and one of Deyeuxia (D. eminens). Despite the number of satDNA loci showing interspecific variation, the general distribution pattern of each satDNA family is maintained. The four satDNA families are AT-rich and associated with DAPI + heterochromatin regions. D2, D3, and D12 have mainly subterminal distribution, while D13 is distributed in intercalary regions. Such conservation of satDNA patterns suggests a not random distribution in genomes, where the variation between species is mainly associated with the array size and the loci number. The presence of satDNA in all species studied suggests a low genetic differentiation of sequences. On the other hand, the variation of the distribution pattern of satDNA has no clear association with phylogeny. This may be related to high differential amplification and contraction of sequences between lineages, as explained by the library model. |
| first_indexed | 2024-12-13T20:37:23Z |
| format | Article |
| id | doaj.art-89768223e24f4a96971bf7be10db8f81 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-13T20:37:23Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-89768223e24f4a96971bf7be10db8f812022-12-21T23:32:16ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-09-011210.3389/fgene.2021.728664728664Chromosomal Differentiation of Deschampsia (Poaceae) Based on Four Satellite DNA FamiliesMaría Laura González0Jorge Oscar Chiapella1Juan Domingo Urdampilleta2Instituto Multidisciplinario de Biología Vegetal (Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba), Córdoba, ArgentinaInstituto de Investigaciones en Biodiversidad y Medioambiente (Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional Del Comahue), Bariloche, ArgentinaInstituto Multidisciplinario de Biología Vegetal (Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba), Córdoba, ArgentinaDiverse families of satellite DNA (satDNA) were detected in heterochromatin regions of Deschampsia. This kind of repetitive DNA consists of tandem repeat sequences forming big arrays in genomes, and can contribute to lineages differentiation. The differentiation between types of satDNA is related to their sequence identity, the size and number of monomers forming the array, and their chromosomal location. In this work, four families of satDNA (D2, D3, D12, D13), previously isolated by genomic analysis, were studied on chromosomal preparations of 12 species of Deschampsia (D. airiformis, D. antarctica, D. cespitosa, D. cordillerarum, D. elongata, D. kingii, D. laxa, D. mendocina, D. parvula, D. patula, D. venustula, and Deschampsia sp) and one of Deyeuxia (D. eminens). Despite the number of satDNA loci showing interspecific variation, the general distribution pattern of each satDNA family is maintained. The four satDNA families are AT-rich and associated with DAPI + heterochromatin regions. D2, D3, and D12 have mainly subterminal distribution, while D13 is distributed in intercalary regions. Such conservation of satDNA patterns suggests a not random distribution in genomes, where the variation between species is mainly associated with the array size and the loci number. The presence of satDNA in all species studied suggests a low genetic differentiation of sequences. On the other hand, the variation of the distribution pattern of satDNA has no clear association with phylogeny. This may be related to high differential amplification and contraction of sequences between lineages, as explained by the library model.https://www.frontiersin.org/articles/10.3389/fgene.2021.728664/fullDeschampsiacytogeneticsrepetitive DNAsatellite DNAFISH |
| spellingShingle | María Laura González Jorge Oscar Chiapella Juan Domingo Urdampilleta Chromosomal Differentiation of Deschampsia (Poaceae) Based on Four Satellite DNA Families Frontiers in Genetics Deschampsia cytogenetics repetitive DNA satellite DNA FISH |
| title | Chromosomal Differentiation of Deschampsia (Poaceae) Based on Four Satellite DNA Families |
| title_full | Chromosomal Differentiation of Deschampsia (Poaceae) Based on Four Satellite DNA Families |
| title_fullStr | Chromosomal Differentiation of Deschampsia (Poaceae) Based on Four Satellite DNA Families |
| title_full_unstemmed | Chromosomal Differentiation of Deschampsia (Poaceae) Based on Four Satellite DNA Families |
| title_short | Chromosomal Differentiation of Deschampsia (Poaceae) Based on Four Satellite DNA Families |
| title_sort | chromosomal differentiation of deschampsia poaceae based on four satellite dna families |
| topic | Deschampsia cytogenetics repetitive DNA satellite DNA FISH |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.728664/full |
| work_keys_str_mv | AT marialauragonzalez chromosomaldifferentiationofdeschampsiapoaceaebasedonfoursatellitednafamilies AT jorgeoscarchiapella chromosomaldifferentiationofdeschampsiapoaceaebasedonfoursatellitednafamilies AT juandomingourdampilleta chromosomaldifferentiationofdeschampsiapoaceaebasedonfoursatellitednafamilies |